Brazilian Journal of Biology,
Journal Year:
2024,
Volume and Issue:
84
Published: Jan. 1, 2024
Abstract
The
global
population
increase
necessitates
the
dire
need
for
ample
food
as
medicine.
Good
health
and
well-being
are
stressed
on
probiotic
functional
foods.
present
study
characterizes
biochemical
molecular
identification
of
potential
lactic
acid
bacteria.
potent
antimicrobial
properties
also
affirm
Lactobacillus
delbrueckii
subsp.
bulgaricus
strain
3286.
Biochemical
analysis
comprises
carbohydrate
fermentation,
tolerance
to
acids
bile
salts,
production
bioactive
compounds,
lecithinase
production,
gelatinase
ripening
ability.
Antibiotic
sensitivity
various
antibiotics
was
assessed
employing
minimum
inhibitory
concentration
(MIC)
E-test.
Strain
resistance
increased
salt
concentrations
coherently
concludes
positive
impact
gut
microbiome
gut-brain
axis
management.
preliminary
assessment
requires
further
in
vitro,
vivo,
silico
commercialization,
market
strategy
utility
supplementation.
can
be
rationalized
sustainable
development
goals
regarding
SDG
3:
good
well-being.
Further,
UNSDG
agenda
2030
ascertains
role
foods
life
longevity
public
management
systems.
Foods,
Journal Year:
2023,
Volume and Issue:
13(1), P. 69 - 69
Published: Dec. 24, 2023
Gluten
consumption
causes
several
immunological
and
non-immunological
intolerances
in
susceptible
individuals.
In
this
study,
the
dextran-producing
Weissella
cibaria
BAL3C-5
its
derivative,
riboflavin-overproducing
strain
C120T,
together
with
a
commercial
bakery
yeast,
were
used
to
ferment
gluten-free
(GF)-doughs
obtained
from
corn
rice
flours
at
two
different
concentrations
supplemented
either
quinoa,
buckwheat,
or
chickpea
obtain
laboratory-scale
GF
bread.
The
levels
of
dextran,
riboflavin,
total
flavins
determined
fermented
breads.
Both
strains
grew
doughs
contributed
especially
those
made
plus
quinoa
(~1
g/100
g).
highest
riboflavin
(350–150
µg/100
g)
flavin
(2.3–1.75
mg/100
observed
though
some
differences
detected
between
various
breads,
suggesting
an
impact
type
flour
used.
safety
assessment
confirmed
lack
pathogenic
factors
bacterial
strains,
such
as
hemolysin
gelatinase
activity,
well
genetic
determinants
for
biogenic
amine
production.
Some
intrinsic
resistance
antibiotics,
including
vancomycin
kanamycin,
was
found.
These
results
indicated
microbiological
both
W.
their
potential
application
baking
produce
This
strain
was
isolated
from
traditionally
(homemade)
fermented
Lithuanian
cherry
tomatoes.
The
genome
consists
of
55
contigs
with
a
total
size
3,326,119
bp,
an
N50
170738,
and
GC%
44.3%.
According
to
the
COG
annotation,
most
these
proteins
were
divided
into
three
categories
related
transcription
(K
category:
307),
amino
acid
transport
metabolism
(E
222),
carbohydrate
(G
268).
No
genes
associated
antimicrobial
resistance
or
virulence
factors
identified.
data
presented
here
can
be
used
in
comparative
genomics
identify
that
may
present
relevant
Lactobacillus
species.
PlasmidFinder
server
revealed
presence
plasmid
pR18
(assessment
number
JN601038)
has
lincomycin
resistance,
transferred
one
bacterium
another,
is
common
plasmids
genera
Bacillus
Lactobacillus.
draft
sequence
have
been
deposited
NCBI
under
Bioproject
accession
PRJNA947394.
Frontiers in Cellular and Infection Microbiology,
Journal Year:
2024,
Volume and Issue:
14
Published: May 21, 2024
With
the
increasing
research
on
exploitation
of
rumen
microbial
resources,
probiotics
have
attracted
much
attention
for
their
positive
contributions
in
promoting
nutrient
digestion,
inhibiting
pathogenic
bacteria,
and
improving
production
performance.
In
past
two
decades,
macrogenomics
has
provided
a
rich
source
new-generation
probiotic
candidates,
but
most
these
"dark
substances"
not
been
successfully
cultured
due
to
restrictive
growth
conditions.
However,
fueled
by
high-throughput
culture
sorting
technologies,
it
is
expected
that
potential
can
be
exploited
large
scale,
applications
medicine
agriculture
explored.
this
paper,
we
review
summarize
classical
techniques
isolation
identification
probiotics,
introduce
development
droplet-based
cell
single-cell
sequencing
identification,
finally
promising
cultureomics
techniques.
The
aim
provide
technical
references
related
technologies
microbiological
promote
further
field
microbiology
research.
Brazilian Journal of Biology,
Journal Year:
2024,
Volume and Issue:
84
Published: Jan. 1, 2024
Abstract
The
global
population
increase
necessitates
the
dire
need
for
ample
food
as
medicine.
Good
health
and
well-being
are
stressed
on
probiotic
functional
foods.
present
study
characterizes
biochemical
molecular
identification
of
potential
lactic
acid
bacteria.
potent
antimicrobial
properties
also
affirm
Lactobacillus
delbrueckii
subsp.
bulgaricus
strain
3286.
Biochemical
analysis
comprises
carbohydrate
fermentation,
tolerance
to
acids
bile
salts,
production
bioactive
compounds,
lecithinase
production,
gelatinase
ripening
ability.
Antibiotic
sensitivity
various
antibiotics
was
assessed
employing
minimum
inhibitory
concentration
(MIC)
E-test.
Strain
resistance
increased
salt
concentrations
coherently
concludes
positive
impact
gut
microbiome
gut-brain
axis
management.
preliminary
assessment
requires
further
in
vitro,
vivo,
silico
commercialization,
market
strategy
utility
supplementation.
can
be
rationalized
sustainable
development
goals
regarding
SDG
3:
good
well-being.
Further,
UNSDG
agenda
2030
ascertains
role
foods
life
longevity
public
management
systems.
