Pathogens,
Journal Year:
2024,
Volume and Issue:
13(11), P. 1022 - 1022
Published: Nov. 20, 2024
The
rapid
and
accurate
detection
of
SARS-CoV-2
in
environmental
settings
is
crucial
for
effective
public
health
management
during
the
COVID-19
pandemic.
This
study
compares
performance
Reverse
Transcription
quantitative
polymerase
chain
reaction
(RT-qPCR)
loop-mediated
isothermal
amplification
(RT-LAMP)
from
100
surface
samples
collected
healthcare
environments.
reference
method,
RT-qPCR,
identified
a
percentage
25%
positive
samples,
while
RT-LAMP
detected
27%
surfaces.
Our
findings
reveal
sensitivity
32%
specificity
75%
RT-LAMP,
with
predictive
value
30%
negative
77%.
overall
accuracy
concordance
RT-qPCR
was
64%
both
methods.
Despite
its
lower
compared
to
had
an
advantage
due
screening
surveillance,
which
particularly
useful
confirming
results.
These
results
underscore
potential
not
only
as
valuable
method
monitoring
but
also
system
control
sanitation
process
ordinary
emergency
conditions,
providing
further
optimization
validation
reliability
routine
surveillance
outbreak
response
efforts.
Cuadernos de Educación y Desarrollo,
Journal Year:
2024,
Volume and Issue:
16(3), P. e3550 - e3550
Published: March 5, 2024
Objetivo:
Analisar
a
associação
entre
ensino
presencial
na
graduação
e
risco
de
adoecimento
por
COVID-19
alunos
uma
universidade,
no
período
agosto
2020
dezembro
2021.
Métodos:
Realizou-se
um
estudo
coorte
retrospectivo
durante
o
referido
período,
com
grupo
exposição
composto
do
1º
ao
9º
Medicina
(presencial)
controle
7º
Direito
(remoto)
mesma
instituição.
Coleta
dados
foi
feita
meio
questionário
online,
aprovado
pelo
Comitê
Ética
em
Pesquisa
(CAAE:
63362022.5.0000.8044).
O
tratamento
estatístico
incluiu
teste
Qui-Quadrado
Pearson
(χ²)
para
variáveis
qualitativas.
Resultados:
A
amostra,
constituída
467
indivíduos,
registrou
54,6%
(255)
casos
COVID-19,
sendo
49,6%
(146)
curso
(controle)
63%
(109)
(exposição).
análise
revelou
relativo
(RR)
1,269
(IC
95%:
1,079
–
1,492,
p=0,0052)
COVID-19.
não
recepção
nenhuma
dose
da
vacina
identificada
3,46%
0,36%
controle.
Houve
redução
aproximadamente
55%
adesão
à
terceira
nos
grupos.
Conclusão:
Os
resultados
indicam
nula
amostra
estudada.
Evidencia-se
que
as
medidas
proteção
adotadas
foram
efetivas
mitigação
contaminação
disseminação
doença,
reforçando
importância
estratégias
preventivas
ambiente
acadêmico,
destacando
papel
crucial
das
segurança
implementadas
analisado.
Microbiology Independent Research Journal (MIR Journal),
Journal Year:
2024,
Volume and Issue:
11(1)
Published: Jan. 1, 2024
ПРЕДПОСЫЛКИ:
Проблема
эффективности
вакцинации
в
отношении
вирусов,
претерпевающих
постоянные
антигенные
изменения
процессе
эволюции,
настоящее
время
решается
за
счет
обновления
состава
вакцин
для
обеспечения
соответствия
циркулирующим
штаммам.
В
случае
внезапного
появления
популяции
вируса,
значительно
отличающегося
от
циркулирующего
(антигенный
шифт
или
дрейф),
нового
каким
стал
SARS-CoV-2,
этот
подход
не
работает.
связи
с
этим
существует
необходимость
создания
универсальной
вакцины,
индуцирующей
Т-клеточный
иммунный
ответ
к
консервативным
антигенным
детерминантам
вируса.
ЦЕЛЬ
ИССЛЕДОВАНИЯ:
Получение
вакцинного
кандидата
на
основе
аттенуированного
гриппозного
вектора
иммунизации
целью
индукции
защиты
гетерологичного
вируса
гриппа
и
коронавируса.
МЕТОДЫ:
На
A/Puerto
Rico/8/1934
(H1N1)
(PR/8/34)
был
сконструирован
рекомбинантный
вектор
FluCoV-N,
содержащий
аттенуирующие
мутации
генах
ns1
nep
экспрессирующий
N-концевую
последовательность
белка
N
(N
1-209
)
SARS-CoV-2.
Для
моделирования
хорьков
через
24
дня
после
однократной
вектором
дозе
9.4
log
10
ЭИД
50
их
заражали
гетерологичным
штаммом
A/Austria/1516645/2022
(H3N2).
проверки
хомяков
коронавируса
проводили
однократную
иммунизацию
8.2
,
а
21
день
инфицировали
РЕЗУЛЬТАТЫ:
Полученный
характеризовался
температурочувствительностью
(ts),
способностью
стимуляции
системы
интерферонов
I
типа
(IFN
I)
безвредностью
животных
при
интраназальном
применении
высокой
дозе.
Однократная
интраназальная
иммунизация
FluCoV-N
приводила
ускоренному
разрешению
инфекции,
вызванной
вирусом
H3N2.
Аналогичная
обеспечивала
снижение
титров
SARS-CoV-2
легких
10000
раз
второй
инфекции
уменьшала
патологию
животных.
ЗАКЛЮЧЕНИЕ:
гриппозным
защищала
животных,
ослабляя
развитие
заболевания,
вызванного
соответственно.
Diagnostics,
Journal Year:
2024,
Volume and Issue:
14(7), P. 720 - 720
Published: March 29, 2024
The
protein-L-utilizing
Förster
resonance
energy
transfer
(LFRET)
assay
enables
mix-and-read
antibody
detection,
as
demonstrated
for
sera
from
patients
with,
e.g.,
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2),
Zika
virus,
and
orthohantavirus
infections.
In
this
study,
we
compared
paired
serum
whole
blood
(WB)
samples
of
COVID-19
SARS-CoV-2
vaccine
recipients.
We
found
that
LFRET
also
detects
specific
antibodies
in
WB
samples.
44
serum–WB
pairs
with
laboratory-confirmed
COVID-19,
showed
a
strong
correlation
between
the
sample
materials.
By
analyzing
89
additional
samples,
totaling
133
results
were
moderately
correlated
enzyme-linked
immunosorbent
collected
to
14
months
after
receiving
diagnosis.
However,
decreased
>14
When
comparing
neutralizing
titers,
emerged
1
This
study
highlights
versatility
detecting
directly
suggests
it
could
be
employed
rapidly
assessing
responses
infectious
agents
or
vaccines.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: July 9, 2024
Abstract
In
2019,
the
novel
SARS-CoV-2
coronavirus
emerged
in
China,
causing
pneumonia
named
COVID-19.
At
beginning,
all
research
efforts
were
focused
on
spike
(S)
glycoprotein.
However,
it
became
evident
that
nucleocapsid
(N)
protein
is
pivotal
viral
replication,
genome
packaging
and
evasion
of
immune
system,
highly
immunogenic,
which
makes
another
compelling
target
for
antibody
development
alongside
protein.
This
study
construction
single
chain
fragments
variable
(scFvs)
libraries
from
SARS-CoV-2-infected
patients
to
establish
a
valuable,
immortalized
extensive
antibodies
source.
We
used
Intracellular
Antibody
Capture
Technology
select
panel
scFvs
against
N
The
whole
scFv
was
expressed
characterized
both
as
intrabodies
recombinant
proteins.
ScFvs
then
divided
into
2
subgroups:
those
exhibited
high
binding
activity
when
yeast
or
mammalian
cells
intrabodies,
purified
proteins,
displaying
affinity
nanomolar
range.
represents
platform
potential
diagnostic
applications.
Frontiers in Virology,
Journal Year:
2024,
Volume and Issue:
4
Published: Oct. 3, 2024
In
the
past
25
years,
world
has
witnessed
outbreaks
of
illnesses
in
humans
from
three
different
coronaviruses.
Both
SARS-CoV
outbreak
2003
and
MERS-CoV
2013
resulted
overall
low
fatalities
part
due
to
inefficient
human-to-human
spread
each
virus.
contrast,
SARS-CoV-2,
which
emerged
2019,
was
highly
efficient
at
caused
a
global
pandemic
resulting
millions
casualties.
Zoonotic
transmission
viruses,
including
coronaviruses,
poses
an
ongoing
threat
that
cannot
be
ignored.
this
review,
we
have
focused
on
diagnostics
therapeutics
fronts
using
SARS-CoV-2
as
model.
Specifically,
selected
proteins
associated
with
virus
particles
targets
discussed
various
platform
technologies.
These
insights
hold
potential
inform
development
more
effective
vaccines
not
only
for
but
also
future
viral
pandemics,
thus
contributing
health
broader
scale.
Veterinary and Animal Science,
Journal Year:
2024,
Volume and Issue:
26, P. 100408 - 100408
Published: Nov. 6, 2024
Following
the
emergence
of
SARS-CoV-2
in
late
2019,
several
species
domestic
and
wild
animals
have
been
found
to
be
susceptible
infection
through
experimental
inoculation
animal
surveillance
activities.
Detection
specific
antibodies
is
an
important
tool
since
viral
shedding
can
only
detected
for
a
short
period
time.
In
this
study,
convenience
serum
samples
were
collected
from
691
cattle,
698
sheep,
707
goats
regions
United
States,
between
2019
2022.
The
evaluated
presence
using
two
commercial
enzyme-linked
immunosorbent
assays
(ELISA);
one
based
on
inhibition
receptor-binding
domain
(sVNT)
other
nucleocapsid
protein
(N-ELISA)
SARS-CoV-2.
Positive
sVNT
additionally
conventional
virus
neutralization
test
(VNT)
employing
Wuhan-like
USA/WA1/2020
isolate.
Our
results
indicate
that
∼1
%
(6/691)
∼2
(13/698)
∼2.5
(18/707)
goat
positive
when
sVNT,
whereas
∼4
cattle
(25/691)
sheep
(27/698),
2.5
tested
with
N-ELISA.
None
or
had
detectable
neutralizing
antibody
activity
(<1:8)
against
isolate
by
VNT.
low
seropositivity
U.S.,
indicating
importance
continue
monitoring
prevalence
are
close
contact
humans.
Pathogens,
Journal Year:
2024,
Volume and Issue:
13(11), P. 1022 - 1022
Published: Nov. 20, 2024
The
rapid
and
accurate
detection
of
SARS-CoV-2
in
environmental
settings
is
crucial
for
effective
public
health
management
during
the
COVID-19
pandemic.
This
study
compares
performance
Reverse
Transcription
quantitative
polymerase
chain
reaction
(RT-qPCR)
loop-mediated
isothermal
amplification
(RT-LAMP)
from
100
surface
samples
collected
healthcare
environments.
reference
method,
RT-qPCR,
identified
a
percentage
25%
positive
samples,
while
RT-LAMP
detected
27%
surfaces.
Our
findings
reveal
sensitivity
32%
specificity
75%
RT-LAMP,
with
predictive
value
30%
negative
77%.
overall
accuracy
concordance
RT-qPCR
was
64%
both
methods.
Despite
its
lower
compared
to
had
an
advantage
due
screening
surveillance,
which
particularly
useful
confirming
results.
These
results
underscore
potential
not
only
as
valuable
method
monitoring
but
also
system
control
sanitation
process
ordinary
emergency
conditions,
providing
further
optimization
validation
reliability
routine
surveillance
outbreak
response
efforts.
