Comprehensive quantitative evaluation and mechanism analysis of influencing factors on yield and quality of cultivated Gastrodia elata DOI Creative Commons

Xiaojun Jiang,

Jing Dai, Yi Huang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 14, 2024

Abstract Gastrodia elata (G. elata) is a dual-purpose herb for medicine and food. Wild resources are depleted, there significant decrease in yield or quality when they cultivated artificially. However, what factors led to the decline still unclear. In this study, based on comprehensive data under multiple production regions, hierarchical partitioning partial least squares path modeling were used first time quantitatively evaluate dominant influencing mechanism of G. elata. The results showed that Armillaria relative abundance, soil available potassium temperature seasonality key factors. Their independent effects 74.14%, 24.78%, 20.36% yield, 36.83%, 25.63%, 21.30% quality, respectively. Plant subspecies directly determined (P < 0.01). Soil physical properties affected chemical properties, which turn biological ultimately yields 0.05). Meanwhile, by Our study contributes novel insight optimize cultivation strategies elata, providing more knowledge improving achieving large-scale greenhouse cultivation.

Language: Английский

Genome-wide identification of carboxyesterase family members reveals the function of GeCXE9 in the catabolism of parishin A in Gastrodia elata DOI

Yaxing Yan,

Mei Jiang,

Xiao Wang

et al.

Plant Cell Reports, Journal Year: 2025, Volume and Issue: 44(2)

Published: Jan. 30, 2025

Language: Английский

Citations

0
Analysis of Suitable Cultivation Sites for Gastrodia elata Using GIS: A Comparison of Various Classification Methods DOI Creative Commons
Gyeong-Mi Tak,

Chong‐Kyu Lee,

Seonghun Jeong

et al.

Applied Sciences, Journal Year: 2025, Volume and Issue: 15(3), P. 1511 - 1511

Published: Feb. 2, 2025

Gastrodia elata has been a valuable medicinal resource in the East for approximately 3000 years. In South Korea, G. is cultivated open-fields or greenhouses near residential areas. However, due to severe continuous damage, cultivation sites need be frequently relocated, leading shortage of available Alternatively, farmers are focusing on mountain cultivation. This study analyzed suitable mountainous areas using geographic information system (GIS) and applied various classification methods identify their characteristics similarities. The analysis showed that Natural Breaks (Jenks) method maximized differences between grades, whereas Quantile reclassified area relatively high proportion. contrast, Equal Interval unsuitable lower proportion, Geometric best demonstrated extreme-temperature regions as sites. Among methods, yielded most similar results. These findings provide critical methodological outcomes sustainable agriculture forestry. Future empirical research application climate change scenarios necessary enhance sustainability industry.

Language: Английский

Citations

0
Comparative transcriptome analysis reveals the potential mechanism of seed germination promoted by Trametenolic acid in Gastrodia elata Blume DOI Creative Commons
Jie Liu,

Wang-Yang Ye,

Danyang Wang

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Abstract Background Gastrodia elata Blume (GEB) is a potential medicinal and edible plant with several active components pharmacological activity that has high application value in medicine the food business. However, natural conditions, GEB seed very low germination rate depends on two specific fungi, germinal nutritive to complete process growth. Armillaria mellea, while acting as nutrient supplier, actually inhibits of seeds. Mycena strains, main germinating can facilitate but cannot support subsequent growth development GEB. It requires symbiotic interactions mellea obtain nutrients for its complex life cycle. Our previous studies have shown Trametenolic acid (TA) effectively promote The aim this study was use transcriptome sequencing further understand mechanism triggered by TA GEB, order lay groundwork developing new germination-growth system mellea. Results untreated group (Group A0) did not germinate test. high-dose TA-treated B), low-dose C), non-symbiotic A) had rates 85.01%, 61.18% 27.39%, respectively. This indicates treatment induce symbiosis seeds significantly increase rates. Transcriptome (RNA-seq) Groups A, B, C identified 86843 annotated genes. There were more down-regulated genes than up-regulated genes, 3912, 2518, 814 differentially expressed (DEGs) between B C, DEGs mainly involved DNA transcription factors, cell wall actions, plant-pathogen interactions, phenylpropanoid biosynthesis, phytohormone signal transduction, starch-sucrose metabolism pathways. Six confirmed using qRT-PCR: Down-regulated lignin biosynthesis pathway include MYB4 4CL, GA20ox1 gibberellin also down-regulated. Up-regulated interaction are AIB WRKY51, MYB44 showing up-regulation. transcriptomics results supported these expression patterns. Lignin, GA, abscisic (ABA) levels analyzed protocorms how promotes germination. showed groups lower ABA levels, higher GA compared A. revealed certain play crucial role promoting through TA, regulating gene alter content hormone breaking dormancy, facilitating seed-fungus relationships Conclusion regulate related hormones, leading an decrease content. helps break dormancy Additionally, enhance GEB's defense response against fungi improves overcoming technical challenges associated fungus. establishes laying foundation research molecular mechanisms

Language: Английский

Citations

0
Research on the signaling pathways related to the intervention of traditional Chinese medicine in Parkinson's disease:A literature review DOI Creative Commons

Xiaxia Jin,

Wendi Dong,

Kaile Chang

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 326, P. 117850 - 117850

Published: Feb. 6, 2024

Language: Английский

Citations

3
Features of bacterial and fungal communities in the rhizosphere of Gastrodia elata cultivated in greenhouse for early harvest DOI Creative Commons

Nguyen Van Khanh,

Swarnalee Dutta,

Chang‐Su Kim

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: April 24, 2024

Symbiotic microbes are essential for developing and growing

Language: Английский

Citations

3
Discovery of seed germinating fungi (Mycetinis scorodonius) from Gastrodia elata Bl. f. glauca S. chow in Changbai Mountain and examination of their germination ability DOI Creative Commons
Xinyu Yang, Yugang Gao, Zhaochun Li

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: May 28, 2024

Abstract Multi-generational asexual reproduction of Gastrodia elata Bl. will cause seedling species degeneration. Sexual seed is an effective method to solve the problem The development seeds cannot be separated from germination fungus. However, there are few strains fungus in production, and also degradation application for many years. It very important sexual isolate more new excellent origin. This study used f. glauca S. chow vegetative propagation corms capture its symbiotic fungus, comprehensively identified by colony morphology, ITS, sporocarps regeneration function, compared growth characteristics ability with other ( Mycena purpureofusca , dendrobii osmundicola ). was isolated named GYGL-1. After comprehensive identification, GYGL-1 Mycetinis scorodonius . Compared has fast speed, vigorous growth, high seeds. Innovated isolation obtained regenerated discovered that a function germinating seeds, enriching resource library

Language: Английский

Citations

3
Decrease in beneficial bacteria and increase in harmful bacteria in Gastrodia seedlings and their surrounding soil are mainly responsible for degradation of Gastrodia asexual propagation DOI Creative Commons
Xi Wang, Yugang Gao, Pu Zang

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 6, 2024

Introduction Asexual reproduction of Gastrodia elata Bl. f. glauca S. chow (GeB) produces degeneration with increasing number GeB. Therefore, we analyzed the microorganisms GeB seedlings and surrounding soil by Illumina Miseq high-throughput sequencing technology. Methods In this study, technology was applied to analyze types quantities in first third generations asexual reproduction, isolated identified dominant strains screened antagonistic bacteria its pathogenic fungi, evaluated effects beneficial on production performance planted Results With an increase reproductive generations, fungi increased, decreased. Pseudomonas sp., Agrobacterium rhizomes , Herbaspirillum hiltneri were generation, Trichoderma harzianum, Penicillium viridiatum, Fusarium oxysporum Novosphingobium sp. Were generation. Antagonistic three bacterial screened. conclusion, significantly improved Discussion our findings suggested that change as increases, disrupts microecological balance endophytic microbiomes.This study provides a theoretical basis for degradation

Language: Английский

Citations

2
Comprehensive quantitative evaluation and mechanism analysis of influencing factors on yield and quality of cultivated Gastrodia elata DOI Creative Commons

Xiaojun Jiang,

Jing Dai, Yi Huang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 14, 2024

Abstract Gastrodia elata (G. elata) is a dual-purpose herb for medicine and food. Wild resources are depleted, there significant decrease in yield or quality when they cultivated artificially. However, what factors led to the decline still unclear. In this study, based on comprehensive data under multiple production regions, hierarchical partitioning partial least squares path modeling were used first time quantitatively evaluate dominant influencing mechanism of G. elata. The results showed that Armillaria relative abundance, soil available potassium temperature seasonality key factors. Their independent effects 74.14%, 24.78%, 20.36% yield, 36.83%, 25.63%, 21.30% quality, respectively. Plant subspecies directly determined (P < 0.01). Soil physical properties affected chemical properties, which turn biological ultimately yields 0.05). Meanwhile, by Our study contributes novel insight optimize cultivation strategies elata, providing more knowledge improving achieving large-scale greenhouse cultivation.

Language: Английский

Citations

0