Emerging Microbes & Infections, Journal Year: 2024, Volume and Issue: 13(1)
Published: Sept. 11, 2024
Carbapenem-resistant
Language: Английский
Drug Resistance Updates, Journal Year: 2024, Volume and Issue: 74, P. 101083 - 101083
Published: March 29, 2024
Carbapenem-resistant Klebsiella pneumonia (CRKP) is a global threat that varies by region. The distribution, evolution, and clinical implications of the ST11 CRKP clone remain obscure. We conducted multicenter molecular epidemiological survey using isolates obtained from 28 provinces municipalities across China between 2011 2021. integrated sequences public databases performed genetic epidemiology analysis CRKP. Among CRKP, KL64 serotypes exhibited considerable expansion, increasing 1.54% to 46.08% Combining our data with databases, phylogenetic phylogeography analyses indicated appeared in Americas 1996 spread worldwide, key clones progressing China's southeastern coast inland 2010. Global showed has evolved virulent, resistant clade notable regional spread. Single-nucleotide polymorphism (SNP) identified BMPPS (bmr3, mltC, pyrB, ppsC, sdaC) as marker for this clade. SNP associated high mortality strong anti-phagocytic competitive traits vitro. high-risk subclone expansion potential survival advantages, probably owing factors.
Language: Английский
Citations
25
Virulence, Journal Year: 2024, Volume and Issue: 15(1)
Published: May 12, 2024
ST11 is the most common lineage among carbapenem-resistant Klebsiella pneumoniae (CRKP) infections in Asia. Diverse morphotypes resulting from genetic mutations are associated with significant differences microbial characteristics K. isolates. Here, we investigated determinants and critical distinct of CRKP. An ST11-KL47 CRKP isolate carrying a pLVPK-like virulence plasmid was isolated patient bloodstream infection; had "mcsw" morphotype. Two ("ntrd" "msdw") were derived this strain during vitro passage. Whole genome sequencing used to identify that cause Transmission electron microscopy, antimicrobial susceptibility tests, growth assays, biofilm formation, membrane permeability RNA-seq analysis investigate specific different Compared parental mcsw morphotype, ntrd morphotype resulted mutation genes involved capsular polysaccharide biosynthesis (wza, wzc, wbaP), result validated by gene knockout experiments. This showed capsule deficiency lower potential, but higher production. By contrast, msdw displayed competition increased chlorhexidine polymyxin B. Further analyses indicated these caused interruption sigma factor rpoN insertion deletion gene, which attenuated integrity presumably downregulating phage shock protein operon. These data expand current understanding genetic, virulence, resistance
Language: Английский
Citations
5
UMI Medical Journal, Journal Year: 2024, Volume and Issue: 9(1), P. 67 - 82
Published: July 15, 2024
Latar Belakang: Sifat co-transfer Gen pengkode hipervirulensi (hvKP) dan resistensi antibiotik (MDR-KP) Klebsiella Pneumoniae memfasilitasi konvergensi, membentuk superbug yang berpotensi menyebabkan wabah dengan gejala berat tak terobati. Untuk mengidentifikasi tema riset terbaru mengenai hvKP-MDR-KP, digunakan analisis berbasis co-occurance dari VOSviewer. Studi ini membantu mengubah metadata publikasi menjadi visualisasi kata kunci sesuai penelitian atau cluster pada disiplin ilmu tertentu, dianalisis keilmuan. Isi: Riset hvKP MDR-KP mulai meningkat sejak tahun 2020, didominasi negara China (38 %), diteliti di berbagai bidang, terutama bidang mikrobiologi (41%), infeksi (38%) farmakologi (28%). Dari 39 artikel terpilih co-occurance, 5 saling terkait. Kesimpulan: Resistensi Carbapenem, prevalensi pneumoniae merupakan yg paling aktual, sehingga sangat disarankan terkait selanjutnya.
Citations
0
Emerging Microbes & Infections, Journal Year: 2024, Volume and Issue: 13(1)
Published: Sept. 11, 2024
Carbapenem-resistant
Language: Английский
Citations
0