Computational Approaches to Elucidate the Mechanism of Serine–Threonine Kinase (AKT1) Inhibition by Streptococcus pyogenes Exotoxin Superantigen‐Derived Peptides: Pioneering Cancer Therapy DOI Open Access

Nachammai Kathiresan,

Prajisha Jayaprakash, Langeswaran Kulanthaivel

et al.

Peptide Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 13, 2024

ABSTRACT A serine–threonine kinase 1 (AKT1) plays a significant role in biological processes, including cell division, survival, glucose metabolism, and apoptosis pathway. It interacts with numerous growth factors external stimuli, types of human cancer have been associated its activation. Bacterial toxins the potential to eradicate tumors can be utilized for producing bacterial‐based vaccines. One few bacterial species recognized superantigen exotoxins is Streptococcus pyogenes . Superantigen produced by S. , such as SPEA, SPEB, SPEC, which activate CD4 + cells cause release cytokines. This study aims explore various computational methods inhibiting AKT1 using exotoxin peptides from specifically SPEC. Identified were analyzed allergencity, toxicity, hemolytic, physicochemical, anticancer tools The modeled PEP‐FOLD validated Ramachandran plot analysis Procheck server. Protein–peptide docking was conducted HDOCK, followed an binding free energies complexes. Molecular dynamics simulations employed investigate structural changes within AKT1‐peptide Results indicate that identified peptide demonstrates strong affinities favorable interaction profiles AKT1. stability observed throughout suggests effects mediated through these

Language: Английский

Anticancer peptides as novel immunomodulatory therapeutic candidates for cancer treatment DOI Creative Commons

Apurva Sood,

V.V. Jothiswaran,

Amrita Singh

et al.

Exploration of Targeted Anti-tumor Therapy, Journal Year: 2024, Volume and Issue: 5(5), P. 1074 - 1099

Published: Aug. 21, 2024

Cancer remains a concern after years of research in this field. Conventional therapies such as chemotherapy, radiation, and surgery are available for cancer treatment, but they characterized by various side effects. There several immunological challenges that make it difficult the immune system conventional to treat cancer. Some these include heterogeneity, resistance medicines, relapse. Even advanced treatments like checkpoint inhibitors (ICIs), which revolutionized have associated toxicity further necessitate exploration alternative therapies. Anticancer peptides (ACPs) offer promising potential cancer-fighting agents address treatment resistance, tumor metastasis. Although exist components defense plants, animals, fungi, etc., can also be created synthetically used new measure. These possess properties them appealing therapy, apoptosis induction, inhibition angiogenesis, cell membrane breakdown with low toxicity. Their capacity specifically target cells selectively holds promise enhancing environments well improving patients’ quality life. This review provides detailed insights into different prospects ACPs, including their characterization, use immunomodulatory mechanistic details addressing existing strategies. In conclusion, ACPs novel therapeutics due specificity fewer effects than

Language: Английский

Citations

3
Design, Synthesis, and Anticancer and Antibacterial Activities of Quinoline-5-Sulfonamides DOI Creative Commons
Andrzej Zięba, Dominika Pindjaková, Małgorzata Latocha

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(17), P. 4044 - 4044

Published: Aug. 26, 2024

A series of new unique acetylene derivatives 8-hydroxy- and 8-methoxyquinoline- 5-sulfonamide

Language: Английский

Citations

3
Identification of a novel antimicrobial peptide from amphioxus ribosomal protein L27 DOI
Ying Chen, Mengmeng Yi,

Yunsheng Wang

et al.

Fish & Shellfish Immunology, Journal Year: 2024, Volume and Issue: 157, P. 110063 - 110063

Published: Nov. 30, 2024

Language: Английский

Citations

1
A Zeolitic Imidazolate Framework-Based Antimicrobial Peptide Delivery System with Enhanced Anticancer Activity and Low Systemic Toxicity DOI Creative Commons
Jingwen Jiang,

Kaderya Kaysar,

Yanzhu Pan

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(12), P. 1591 - 1591

Published: Dec. 13, 2024

Background: The clinical efficacies of anticancer drugs are limited by non-selective toxic effects on healthy tissues and low bioavailability in tumor tissue. Therefore, the development vehicles that can selectively deliver release at site is critical for further improvements patient survival. Methods: We prepared a CEC nano-drug delivery system, CEC@ZIF-8, with zeolite imidazole framework-8 (ZIF-8) as carrier, which achieve response folate receptor (FR). characterized this system terms morphology, particle size, zeta potential, infrared (IR), x-ray diffraction (XRD), transcriptome analysis, examined vitro cytotoxicity cellular uptake properties CEC@ZIF-8 using cervical cancer cells. Lastly, we established TC-1 tumor-bearing mouse model evaluated its vivo anti-cervical activity. Results: nano-delivery had favorable biocompatibility, heat stability, pH responsiveness, loading efficiency 12%, hydrated size 174 ± 5.8 nm, potential 20.57 mV, slow massive drug an acidic environment (pH 5.5), whereas was 6% neutral 7.4). At same time, confocal imaging cell viability assays demonstrated greater intracellular accumulation more potent against cells compared to free CEC. mechanism analyzed series analyses, revealed NPs differentially regulate expression levels 1057 genes cells, indicated enriched pathways were mainly cycle apoptosis-related via enrichment analysis differential genes. Flow cytometry showed inhibited growth HeLa arresting G0/G1 phase. also induced apoptosis rates than CEC, while unloaded ZIF-8 little inherent pro-apoptotic Furthermore, reactive oxygen species (ROS) upregulated ROS inhibitors caspase reversed NPs-induced apoptosis. Finally, reduced rate xenograft tumors mice without systemic toxicity observed cisplatin treatment. Conclusions: significantly enhanced effect both vitro, providing promising applications management. work demonstrates CEC-loaded nanoparticles selective destruction tissues.

Language: Английский

Citations

1
Cancer-Targeting Applications of Cell-Penetrating Peptides DOI Open Access
Liliana Marisol Moreno‐Vargas, Diego Prada‐Gracia

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 2 - 2

Published: Dec. 24, 2024

Cell-penetrating peptides (CPPs) offer a unique and efficient mechanism for delivering therapeutic agents directly into cancer cells. These can traverse cellular membranes, overcoming one of the critical barriers in drug delivery systems. In this review, we explore recent advancements application CPPs treatment, focusing on mechanisms, strategies, clinical potential. The review highlights use CPP-drug conjugates, CPP-based vaccines, their role targeting inhibiting tumor growth.

Language: Английский

Citations

1
Skin mucus extract derived from Channa punctatus, Channa striatus, and Heteropneustes fossilis induces apoptosis and suppresses proliferation in human adenocarcinoma cells via ROS mediated pathway and cell cycle arrest DOI Creative Commons

Farheen Jameel,

Priyanka Agarwal, Rumana Ahmad

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 12, 2024

Abstract Purpose Skin mucus of freshwater fish species living in muddy habitats possesses the ability to inhibit growth human pathogens, and may also have potential cancer cells. This study first explored anti-cancer activity skin acetic extracts Channa punctatus (CPMA), striatus (CSMA) Heteropneustes fossilis (HFMA) against lung adenocarcinoma. Methods The cytotoxicity extract(s) on A549 NRK-52E cells were evaluated using MTT assay morpholological analysis Nuclear condensation, ROS generation, apoptosis cell cycle was performed protein profiles via. SDS-PAGE. Results SDS-PAGE showed 15.2–69.1, 14.3–70.2 14- 97.5 kDa proteins CPMA, CSMA HFMA respectively. inhibited cellular proliferation a dose-dependent manner, with IC50 values 619 591 (CSMA), 538 µg/mL (HFMA). Morphological changes cells, presence condensed nuclei intracellular production increased dose dependently order > CSMA. Early late apoptotic observed at low high doses Moreover, G2/M arrest found CPMA CSMA, while both dependent manner treated Interestingly, no toxicity healthy Conclusion possess highest cytotoxic effect followed by then which directly corresponds range MW present therein.

Language: Английский

Citations

0
Activity of protegrin-1 against mouse Ehrlich ascites carcinoma <i>in vitro</i> and <i>in vivo</i> DOI
Alena E. Rudel, Т. А. Филатенкова, Maria S. Zharkova

et al.

Medical academic journal, Journal Year: 2024, Volume and Issue: 24(2), P. 117 - 124

Published: Oct. 29, 2024

BACKGROUND: The problem of multidrug resistance in cancer treatment creates an urgent demand for developing new effective antitumor agents. Due to the unusual mechanism recognizing and damaging tumor cells, antimicrobial peptides are considered as possible prototypes designing such therapeutics. AIM: This work was aimed compare potential promising membranolytic peptide protegrin-1 vitro vivo Ehrlich ascites carcinoma mice model. MATERIALS AND METHODS: We used two variants model by inducing a solid or form. In first case, were injected with twice week three weeks, second injections provided every other day six days. activity against isolated cells analyzed using MTT-test. RESULTS: Protegrin-1 demonstrated high vitro, but had no significant effect on lifespan bearing form at dosing administration regimens we used. However, caused decrease volume number fluid. CONCLUSIONS: retains its properties vivo, it may be presumed, that effectively suppress growth requires more frequent prolonged compared conventional antibiotics, which adopted regimen this study.

Language: Английский

Citations

0
Harnessing bacterial metabolites for enhanced cancer chemotherapy: unveiling unique therapeutic potentials DOI
Aroni Chatterjee, Rajni Khan, Triparna Mukherjee

et al.

Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(11)

Published: Oct. 29, 2024

Language: Английский

Citations

0
Antimicrobial Peptides from Frogs of the Glandirana Genus DOI Creative Commons

Frederick Harris,

David A. Phoenix,

Sarah R. Dennison

et al.

Biologics, Journal Year: 2024, Volume and Issue: 4(4), P. 444 - 507

Published: Dec. 8, 2024

Glandirana is a genus of frogs that includes G. rugosa, emeljanovi, minima, tientaiensis, susurra, nakamurai and reliquia. These produce antimicrobial peptides (AMPs), which are endogenous antibiotics possess antibacterial, antifungal, antiviral anti-endotoxin activity help keep the hosts free from infections. In these activities, microbial death promoted by membranolytic mechanisms mediated cationic charge amphiphilic α-helical structures peptides. general, selective for microbes, showing low levels hemolytic cytotoxic activity, as well possessing other biological including anticancer, antioxidative insulinotrophic action. this review, brief overview AMPs with focus on those amphibians provided, along phylogeny nomenclature genus. This review then provides comprehensive, in-depth description activities all produced known period 1994 to 2024. detailed discussion structure/function relationships involved in membrane interactions drive comparisons between same frog across Based their properties, have been proposed investigation potential therapeutic agents, such treatment cancers diabetes, agents areas, crop protection, food industry oral hygiene.

Language: Английский

Citations

0
Role of Peptides in Oral Cancer DOI
Aparna Ramachandran,

B. Saravanan,

Debashish Dhar

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0