Diversity and functional traits based indigenous rhizosphere associated phosphate solubilizing bacteria for sustainable production of rice DOI Creative Commons

Maria Rasul,

Mahreen Yahya,

Muhammad Suleman

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 13, 2024

Introduction Rice, particularly Basmati rice, holds significant global importance as a staple food. The indiscriminate use of phosphate-based fertilizers during rice production has led to high residual levels these chemicals in soil, impacting soil health and fertility. This study aimed address this challenge by investigating the potential phosphate solubilizing bacteria (PSB) improving fertility boosting growth rice. Methods Using amplicon-based 16S rDNA sequencing, bacterial isolation cultivation, conducting greenhouse field experiments, PSB localization, we optimized search for inoculants enhance growth. Results discussion Rice rhizosphere prokaryote communities showed differences microbial diversity composition between basmati non-basmati cultivated areas. Dominant phyla included Proteobacteria, Acidobacteria, Actinobacteria, Firmicutes, with Actinobacteria Proteobacteria playing crucial role nutrient recycling. Isolation optimization strains, including Acinetobacter sp. MR5 Pseudomonas R7, were carried out microcosm studies confirmed their efficacy increasing available phosphorus concentration. Response surface methodology revealed relative factors such pH, inoculum density incubation temperature maximising solubilization. Microplot experiments demonstrated effectiveness promoting growth, increases plant height, tiller number, biomass, grain yield compared uninoculated controls. A consortium proved superior single-strain inoculants, even reduced chemical fertilizer application. Field trials at several growing sites positive impact on yield, availability, uptake. competence persistence inoculated strains was FISH BOX Polymerase Chain Reaction (BOX-PCR). work highlights PSB-based biofertilizers improve fertility, promote sustainable reduce negative environmental impacts fertilizers. Future research would focus scaling up findings widespread adoption agriculture exploring applicability other crops agroecosystems.

Language: Английский

Nematicidal and plant growth-promoting rhizobacteria: a sustainable strategy for controlling Tylenchulus semipenetrans and enhancing citrus growth DOI

Btissam Zoubi,

Mohamed Hijri, Fouad Mokrını

et al.

International Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Language: Английский

Citations

1
Rhizospheric Bacteria of Cover Legumes from Acidic Soils Are Capable of Solubilizing Different Inorganic Phosphates DOI Creative Commons
Winston Franz Ríos-Ruiz,

Roy D. Casique-Huamanguli,

Renzo Alfredo Valdez-Nuñez

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(6), P. 1101 - 1101

Published: May 29, 2024

Due to its adsorption with aluminum and iron hydroxides, phosphorus viability is low in acidic soils; thus, the aim of this study was isolate identify bacteria from rhizosphere four legumes growing soils Cumbaza Sub-basin, San Martín, Peru, as well characterize their ability solubilize phosphate phosphate. The isolation process conducted on TSA medium isolates were classified based origin morphocolonial characteristics, bacillary shape being most frequent, followed by cocci. To assess solubilization phosphates, liquid GELP employed. Sixteen strains selected, among which three stood out for effectiveness solubilizing AlPO4 (Sfcv-098-02, 22.65 mg L−1; Sfc-093-04, 26.50 Sfcv-041-01-2, 55.98 L−1) one FePO4 (Sfcr-043-02, 32.61 L−1). These molecularly characterized, identified Enterobacter sp., Pseudomonas Staphylococcus sp. Additionally, a decrease pH observed reactions, values ranging 5.23 3.29, enhanced solubilization. This suggests that selected could be used improve availability agricultural soils.

Language: Английский

Citations

4
Evaluating Rhizobacterial Antagonists for Controlling Cercospora beticola and Promoting Growth in Beta vulgaris DOI Creative Commons

Zakariae El Housni,

Said Ezrari, Nabil Radouane

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(4), P. 668 - 668

Published: March 27, 2024

Cercospora beticola Sacc. is an ascomycete pathogen that causes leaf spot in sugar beets (Beta vulgaris L.) and other related crops. It can lead to significant yield losses if not effectively managed. This study aimed assess rhizosphere bacteria from beet soil as a biological control agent against C. evaluate their effect on B. vulgaris. Following dual-culture screening, 18 exhibiting over 50% inhibition were selected, with 6 of them demonstrating more than 80% control. The identified by sequencing the 16S rRNA gene, revealing 12 potential species belonging genera, including Bacillus, which was represented 4 species. Additionally, biochemical molecular properties characterized depth, well plant growth promotion. PCR analysis genes responsible for producing antifungal metabolites revealed 83%, 78%, 89%, 56% selected possessed bacillomycin-, iturin-, fengycin-, surfactin-encoding genes, respectively. Infrared spectroscopy confirmed presence lipopeptide structure bacterial supernatant filtrate. Subsequently, assessed plants controlled conditions. exhibited notable capabilities, promoting both roots shoots, resulting increases root length weight shoot length. A field experiment four candidates demonstrated good performance compared difenoconazole fungicide. These played role disease control, achieving maximum efficacy 77.42%, slightly below 88.51% attained difenoconazole. Additional trials are necessary verify protective growth-promoting effects these candidates, whether applied individually, combined consortia, or integrated chemical inputs crop production.

Language: Английский

Citations

2
Enhanced phosphorus availability and cadmium remediation using phosphate-solubilizing bacteria-loaded biochar in contaminated soils DOI Creative Commons
Zhe Li, Zhe Liu,

Dongmei Wu

et al.

Environmental Technology & Innovation, Journal Year: 2024, Volume and Issue: 36, P. 103878 - 103878

Published: Oct. 28, 2024

Language: Английский

Citations

1
Enhanced Soil Phosphorus Availability and Cadmium Remediation Using Phosphate-Solubilizing Bacteria-Loaded Biochar in Contaminated Soils DOI
Zhe Li, Zhe Liu, Shigeng Chen

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Potential Plant-To-Plant Transmission: Shared Endophytic Bacterial Community Between Ziziphus lotus and Its Parasite Cuscuta epithymum DOI Creative Commons
Nabil Radouane, Khaoula Errafii‬, Salma Mouhib

et al.

Microbial Ecology, Journal Year: 2024, Volume and Issue: 87(1)

Published: Sept. 28, 2024

Language: Английский

Citations

0
How do high phosphate concentrations affect soil microbial communities after a century of ecosystem self‐reclamation? DOI Creative Commons
Amandine Ducousso‐Détrez, Simon Morvan, Joël Fontaine

et al.

Environmental Microbiology Reports, Journal Year: 2024, Volume and Issue: 16(5)

Published: Oct. 1, 2024

Abstract The use of rock phosphate (RP) instead soluble fertilizers is preferred for the development more sustainable agriculture. However, impact high concentrations in RP on bacterial and fungal communities remains poorly documented. Thus, next‐generation sequencing was used to characterize soils roots four plant species growing naturally a self‐restored ecosystem, former open‐pit mines where past exploitation generated locally substantial enrichment soil. Our results show that are dominated by Actinobacteria Proteobacteria phyla, while Ascomycota Basidiomycota phyla predominate community. alpha beta diversities both differ significantly between root soil compartments but not affected inputs. Amplicon Sequence Variants (ASVs) indicative RP‐enriched have been identified; among them bacteria representative Streptomyces , Bacillus Mycobacterium or Agromyces . Implications these open new ways reflection understand microbial response following RP‐inputs long‐term restoration, as well formulate microbial‐based bioinoculants agriculture applications based microorganisms better adapted RP.

Language: Английский

Citations

0
Diversity and functional traits based indigenous rhizosphere associated phosphate solubilizing bacteria for sustainable production of rice DOI Creative Commons

Maria Rasul,

Mahreen Yahya,

Muhammad Suleman

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 13, 2024

Introduction Rice, particularly Basmati rice, holds significant global importance as a staple food. The indiscriminate use of phosphate-based fertilizers during rice production has led to high residual levels these chemicals in soil, impacting soil health and fertility. This study aimed address this challenge by investigating the potential phosphate solubilizing bacteria (PSB) improving fertility boosting growth rice. Methods Using amplicon-based 16S rDNA sequencing, bacterial isolation cultivation, conducting greenhouse field experiments, PSB localization, we optimized search for inoculants enhance growth. Results discussion Rice rhizosphere prokaryote communities showed differences microbial diversity composition between basmati non-basmati cultivated areas. Dominant phyla included Proteobacteria, Acidobacteria, Actinobacteria, Firmicutes, with Actinobacteria Proteobacteria playing crucial role nutrient recycling. Isolation optimization strains, including Acinetobacter sp. MR5 Pseudomonas R7, were carried out microcosm studies confirmed their efficacy increasing available phosphorus concentration. Response surface methodology revealed relative factors such pH, inoculum density incubation temperature maximising solubilization. Microplot experiments demonstrated effectiveness promoting growth, increases plant height, tiller number, biomass, grain yield compared uninoculated controls. A consortium proved superior single-strain inoculants, even reduced chemical fertilizer application. Field trials at several growing sites positive impact on yield, availability, uptake. competence persistence inoculated strains was FISH BOX Polymerase Chain Reaction (BOX-PCR). work highlights PSB-based biofertilizers improve fertility, promote sustainable reduce negative environmental impacts fertilizers. Future research would focus scaling up findings widespread adoption agriculture exploring applicability other crops agroecosystems.

Language: Английский

Citations

0