Differences in Microbial Community Structure Determine the Functional Specialization of Gut Segments of Ligia exotica

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 808 - 808

Published: April 2, 2025

Ligia feed on seashore algae and remove organic debris from the coastal zone, thereby playing an important role in intertidal ecosystem. Nevertheless, specific roles of distinct gut segments transit remain unclear. We collected identified exotica specimens coast Aoshanwei, Qingdao, Shandong Province, analyzed their foreguts hindguts for 16S rRNA, metagenomics, metabolomics, proteomics. The concentrations common metabolites, NO3−-N NH4+-N, contents C N were measured. decreased abundances dominant phyla Cyanobacteria but increased Proteobacteria, Firmicutes, Actinobacteria, Planctomycetes Bacteroidetes remained relatively constant. foregut microbiota is involved carbohydrates amino acids metabolism, as well decomposition polysaccharides. hindgut performs a variety functions, including carbohydrate acid fermentation, cell motility, intracellular transport, secretion, vesicular translocation, polysaccharides, disaccharides, oligosaccharides. results omics analyses molecular experiments demonstrated that metabolic processes involving are more active foregut, whereas absorption, assimilation hindgut. Taken together, differences microbial community structure determine functional specialization different segments, i.e., appears to be primary site digesting food, while further absorbs nutrients then excretes them.

Language: Английский

Adaptability of the gut microbiota of the German cockroach Blattella germanica to a periodic antibiotic treatment

Microbiological Research, Journal Year: 2024, Volume and Issue: 287, P. 127863 - 127863

Published: Aug. 3, 2024

High-throughput sequencing studies have shown that diet or antimicrobial treatments impact animal gut microbiota equilibrium. However, properties related to the microbial ecosystem stability, such as resilience, resistance, functional redundancy, must be better understood. To shed light on these ecological processes, we combined advanced statistical methods with 16 S rRNA gene sequencing, prediction, and fitness analyses in of cockroach Blattella germanica subject three periodic pulses antibiotic (AB) kanamycin (n=512). We first confirmed AB did not significantly affect cockroaches' biological fitness, changes were caused by insect physiology alterations. The sex variable was examined for time this species, no differences diversity composition found. comparison dynamics control treated populations revealed (1) treatment decreases completely disrupts co-occurrence networks between bacteria, altering community structure. (2) Although also affected genetic composition, redundancy would explain a smaller effect potential than taxonomic composition. (3) As predicted Taylor's law, generally most abundant taxa lesser extent less taxa. (4) Taxa follow different trends response ABs, highlighting "resistant taxa," which could critical restoration. (5) recovered faster after pulses, suggesting adapts repeated treatments.

Language: Английский

Priming the Nutrition-Gut-Immuno-Oncology Axis

Published: Jan. 1, 2024

Language: Английский