Biofouling,
Journal Year:
2024,
Volume and Issue:
unknown, P. 1 - 11
Published: Dec. 27, 2024
Chromobacterium
violaceum
is
a
pathogenic
bacterium
that
can
infect
humans
and
animals,
yet
the
role
of
its
outer
membrane
vesicles
(OMVs)
in
mediating
pathogenicity
remains
underexplored.
This
study
evaluated
effects
linoleic
acid
(LA)
stearic
(SA)
on
quorum
sensing
(QS)-mediated
violacein
production,
biofilm
formation,
OMV
biogenesis
C.
violaceum.
Our
findings
revealed
2
mM
LA
1
SA
effectively
quench
QS,
leading
to
significant
reduction
biogenesis.
Gene
expression
analysis
confirmed
downregulation
QS-related
genes,
including
cviI,
cviR,
vioA,
vioB,
vioC,
fatty
acid-treated
Additionally,
we
assessed
antimicrobial
activity
violaceum-derived
OMVs
Rhizobium
sp.,
PGPR
observed
marked
bactericidal
treated
OMVs.
suggests
have
potential
as
anti-infective
agents
mitigate
OMV-mediated
virulence
combat
antibiotic
resistance
pathogens.
Natural Product Research,
Journal Year:
2025,
Volume and Issue:
unknown, P. 1 - 8
Published: April 15, 2025
This
study
investigated
the
anti-quorum
sensing
ability
of
Pandanus
tectorius
(PT)
leaf
extract
in
suppressing
virulence
factors
including
biofilm
formation
Chromobacterium
violaceum
under
vitro
conditions.
The
significantly
inhibited
biosynthesis
violacein,
acyl
homoserine
lactone,
chitinase,
exopolysaccharides,
haemolysin,
and
outer
membrane
vesicles
at
sub-MIC
concentrations
1.5,
1,
0.5
mg/ml.
Biofilm
was
reduced
by
81.46%
a
concentration
1.5
PT
also
downregulated
quorum
sensing-associated
genes,
cviI,
cviR,
vioA
C.
violaceum.
GC-MS
analysis
identified
15
major
bioactive
compounds,
which
were
docked
with
cviR
master
regulator
protein.
Among
those,
stigmasterol
demonstrated
efficient
binding
active
site
energy
-8.5
kcal/mol.
Toxicity
assays
confirmed
non-toxic
nature
extract,
highlighting
its
potential
as
safe
agent.
Heliyon,
Journal Year:
2024,
Volume and Issue:
10(11), P. e31898 - e31898
Published: May 27, 2024
Biofilms
are
complex
communities
of
microorganisms
that
cause
systemic
infections,
resistance
development
and
delay
in
healing
wounds.
can
form
various
parts
the
human
body,
such
as
teeth,
lungs,
urinary
tract,
Biofilm
complicates
effects
antibiotics
treating
infections.
In
search
a
cure,
plant-based
phyto
component
was
selected
for
this
investigation
an
anti-quorum-mediated
biofilm
restricting
agent
Gram-negative
BMC Research Notes,
Journal Year:
2024,
Volume and Issue:
17(1)
Published: June 17, 2024
Abstract
Objectives
The
aim
of
this
study
was
to
quantify
and
identify
metabolites
Ice
Nucleation
Active
(INA)
bacteria
as
an
anti-biofilm
agent
against
biofilms
fish
pathogens
such
Aeromonas
hydrophila
Streptococcus
agalactiae.
Results
nucleation
active
bacteria,
which
have
the
ability
catalyze
ice
nucleation,
isolated
from
rainwater
in
previous
studies,
were
used.
All
INA
isolates
tested
several
assays,
including
antimicrobial
test,
uses
streptomycin
positive
control
none
found
test.
As
for
quorum
quenching
assay,
it
that
four
out
ten
able
disturb
communication
system
Chromobacterium
violaceum
wild
type,
used
indicator
bacteria.
On
next
all
Biofilm
Inhibition
Destruction
showed
activity
with
highest
percentage
inhibition
33.49%
by
isolate
A40
A.
77.26%
A19
S.
agalactiae
.
C1
performed
destruction
,
percentages
32.11%
51.88%,
respectively.
GC-MS
analysis,
supernatants
contain
bioactive
compounds
sarcosine
fatty
acids,
are
known
antibiofilm
biofilm-forming
Through
16s
rRNA
sequencing,
identified
Pantoea,
Enterobacter
Acinetobacter
genera.
conclusion,
results
pathogenic
destructing
inhibiting
biofilm
growth.
