Metatranscriptomic analysis reveals the diversity of RNA viruses in ticks in Inner Mongolia, China DOI Creative Commons
Si Su,

Meng-Yu Cui,

Lili Xing

et al.

PLoS neglected tropical diseases, Journal Year: 2024, Volume and Issue: 18(12), P. e0012706 - e0012706

Published: Dec. 11, 2024

Ticks are widely distributed throughout China and the second most prevalent pathogen vectors in world, following only mosquitoes. Tick bites can lead to Lyme disease, forest encephalitis, other illnesses that may result death under severe circumstances. Materials methods: collected from March 2021 May 2023 were pooled used metatranscriptomic analyses gain insight into diversity distribution of tick-borne viruses Inner Mongolia. Next-generation sequencing (NGS) outcomes validated, viral prevalence across distinct tick species was determined through application polymerase chain reaction (PCR) paired with Sanger sequencing.

Language: Английский

Aspartate aminotransferase to platelet ratio correlates with poor prognosis and metabolic alterations in Dabie bandavirus infection DOI Creative Commons

Chunxia Guo,

Ruixue Li, Xia Wang

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 17, 2025

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease a high mortality rate caused by Dabie bandavirus. The aspartate aminotransferase to platelet ratio index (APRI) biomarker of liver injury and inflammation. This study aimed examine the correlation between APRI SFTS prognosis using clinical data analysis attempt explain its prognostic significance through metabolic analysis. Data from hospitalized patients confirmed diagnosis virus infection at Wuhan Union Hospital were retrospectively collected. low groups 1:1 matched propensity score matching (PSM) Fresh plasma was collected on admission used for tests. A total 617 who met inclusion criteria selected Survival revealed that (> 35.3) had substantially higher death than those (≤ 35.3). Receiver operating characteristic showed predictive performance 0.77, 95% CI 0.73-0.80, which superior NLR (area under curve (AUC): 0.65), platelet-to-lymphocyte (AUC: 0.54), systemic immune-inflammation 0.58). value more substantial after PSM before PSM. Metabolomic testing identified several differential serum metabolites, alanine, aspartate, glutamate, glycerophospholipid, tryptophan metabolism being most important pathways. associated relatively in SFTS, survival outcome well-recognized inflammatory scores. Alanine, glutamate are involved progression SFTS.

Language: Английский

Citations

0
Molecular Survey and Phylogenetic Characterization of Tick-Borne Pathogens in Hubei Province, China DOI

Yake Lei,

Ying Dai, Meng Guo

et al.

Vector-Borne and Zoonotic Diseases, Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Background: Tick-borne diseases pose a serious disease burden globally, with pathogens including viruses, bacteria, rickettsia, and spirochetes. This study aims to investigate ticks in Huanggang Suizhou, two regions the highest tick-borne prevalence Hubei, China. Methods: A total of 2,958 were collected, their species diversity was characterized. RNA DNA used for polymerase chain reaction detection sequencing Dabie bandavirus (DBV), spotted fever group rickettsioses (SFGR), Coxiella burnetii, Borrelia, Anaplasma phagocytophilum, Babesia, Bartonella ticks. Molecular evolutionary traits phylogenetic linkages further analyzed. Results: One family, four genera, identified, Haemaphysalis longicornis being predominant species. The overall DBV-positive rate 2.63% from City 0.48% those Suizhou City. Phylogenetic analysis indicated that DBV strains Hubei Province belonged genotypes A-F, high homology sampled local human patients. SFGR-positive significantly higher samples (48.23%) than (8.29%). High levels A. phagocytophilum sequences detected both regions, 99% homologous. Borrelia burgdorferi first time City, very low carriage (0.24%), not No Bartonella, or C. burnetii either location. Conclusions: represents substantial effort Our findings provide references data help develop effective prevention, surveillance, early warning systems.

Language: Английский

Citations

0
HL-IR mediates cinnamaldehyde repellency behavior in parthenogenetic Haemaphysalis longicornis DOI Creative Commons

Ceyan Kuang,

Shi Han, Jie Cao

et al.

PLoS neglected tropical diseases, Journal Year: 2025, Volume and Issue: 19(3), P. e0012877 - e0012877

Published: March 17, 2025

Chemical repellents against arthropods have limitations in terms of toxicity and resistance. Natural plant compounds can be utilized as alternatives for developing environmentally friendly humans animals. A variety essential oils exhibit strong repellent effects ticks; however, the mechanisms action ticks remain unknown. Here, we investigated repellency cinnamaldehyde, a primary compound found cinnamon oil, demonstrated that it affected electrophysiological responses on Haller’s organs parthenogenetic Haemaphysalis longicornis . Transcriptome data indicated cinnamaldehyde response was linked to ionotropic receptor (HL-IR) at various tick developmental stages. HL-IR widely expressed tissues stages according RT-qPCR. In situ hybridization results showed highly ticks. Microinjection double-stranded RNA (dsRNA) reduced transcript levels led significant decreases rate from EAG organ. Experiments using competitive fluorescence binding mutation sites 218ASN critical site HL-IR. We conclude organ expresses HL-IR, this interaction mediates tick-repellent behavior by cinnamaldehyde.

Language: Английский

Citations

0
Real-time quantitative reverse transcription PCR assay for the detection of Nuomin virus - an emerging tick-borne virus DOI

Kairao Hu,

Tingting Liu, Wenbo Xu

et al.

Journal of Virological Methods, Journal Year: 2024, Volume and Issue: 330, P. 115032 - 115032

Published: Sept. 7, 2024

Language: Английский

Citations

0
Ticks Carry Various Antibiotic Resistance Genes and can Serve as Vectors for Their Dissemination and as Reservoirs by Vertical Propagation DOI

Yi Wu,

Yuechen Sun,

Jingpeng Liu

et al.

Environmental Research, Journal Year: 2024, Volume and Issue: 262, P. 119976 - 119976

Published: Sept. 11, 2024

Language: Английский

Citations

0
Pilot Surveillance of <i>Babesia</i>, <i>Borrelia burgdorferi</i>, and <i>Anaplasma phagocytophilum</i> Among Chinese Blood Donors — Xinjiang, Inner Mongolia, and Heilongjiang PLADs, China, 2022–2023 DOI

Huimin Ji,

Le Chang, Ying Yan

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 6(46), P. 1206 - 1210

Published: Jan. 1, 2024

Recently, tick-borne pathogens transmitted through blood transfusions have posed new risks to safety. We developed a quality control system for nucleic acid testing (NAT) Babesia, Borrelia burgdorferi, and Anaplasma phagocytophilum, evaluated five Triplex-NAT kits, conducted external assessments of centers. This study screened 92,700 donors from Heilongjiang, Inner Mongolia, Xinjiang provincial-level administrative divisions during 2022-2023. A donor in Hegang, tested positive marking the first detection this infection Chinese donors. Quality NAT is vital managing pathogen outbreaks. To ensure transfusion safety, screening should be strengthened high-risk areas outside national borders.

Language: Английский

Citations

0
Metatranscriptomic analysis reveals the diversity of RNA viruses in ticks in Inner Mongolia, China DOI Creative Commons
Si Su,

Meng-Yu Cui,

Lili Xing

et al.

PLoS neglected tropical diseases, Journal Year: 2024, Volume and Issue: 18(12), P. e0012706 - e0012706

Published: Dec. 11, 2024

Ticks are widely distributed throughout China and the second most prevalent pathogen vectors in world, following only mosquitoes. Tick bites can lead to Lyme disease, forest encephalitis, other illnesses that may result death under severe circumstances. Materials methods: collected from March 2021 May 2023 were pooled used metatranscriptomic analyses gain insight into diversity distribution of tick-borne viruses Inner Mongolia. Next-generation sequencing (NGS) outcomes validated, viral prevalence across distinct tick species was determined through application polymerase chain reaction (PCR) paired with Sanger sequencing.

Language: Английский

Citations

0