Innovative strategies in the fight against bacterial infections: Phage therapy, nanotechnology, and new antimicrobial agents for multidrug-resistant pathogens
Results in Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown, P. 102304 - 102304
Published: April 1, 2025
Language: Английский
Isolation and characterization of a novel bacteriophage as a biological control agent against multidrug resistant Escherichia coli in compost and agricultural irrigation water
F1000Research,
Journal Year:
2024,
Volume and Issue:
13, P. 1521 - 1521
Published: Dec. 16, 2024
Background
Escherichia
coli
is
a
critical
priority
pathogen
due
to
its
significant
morbidity,
mortality,
and
growing
antimicrobial
resistance,
underscoring
the
urgent
need
for
novel
control
strategies.
This
bacterium
frequently
implicated
in
outbreaks
associated
with
horticultural
products,
particularly
those
cultivated
organic
farming
systems.
The
aim
of
this
study
was
isolate
evaluate
potential
bacteriophage
as
biocontrol
agent
against
E.
compost
agricultural
irrigation
water.
Methods
presence
samples
(n=17)
determined
through
microbiological
assays,
bacterial
identity
confirmed
by
PCR
amplification
phoA
gene.
Antimicrobial
resistance
profiles
isolates
were
assessed
using
disk
diffusion
method.
Bacteriophage
isolation
conducted
from
livestock
fecal
double-layer
agar
technique.
stability
under
varying
pH
levels
temperatures
evaluated,
along
replication
dynamics.
Additionally,
phage’s
efficacy
reducing
populations
water
assessed.
Genomic
sequencing
bioinformatic
analyses
characterize
genetic
profile.
Results
strains
isolated
exhibiting
multidrug
samples.
bacteriophage,
named
Alux-21,
exhibited
at
neutral
retained
viability
both
4°C
40°C
over
six-month
period.
Importantly,
phage
achieved
reduction
counts,
exceeding
3.8
logs
3
water,
demonstrating
superior
compared
previously
reported
phages
similar
substrates.
analysis
absence
virulence-associated,
lysogeny,
antibiotic
genes.
Conclusion
findings
highlight
Alux-21
sustainable
Field
validation
will
be
crucial
establish
scalability
real-world
conditions.
Language: Английский
Genome sequencing of Escherichia coli phage UFJF_EcSW4 reveals a novel lytic Kayfunavirus species
3 Biotech,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: Dec. 15, 2024
Language: Английский