Biodegradation of petroleum hydrocarbon and related molecular microbiology analysis of some fungal species DOI Creative Commons
Huda M. Shakam, Mahmoud A. Gaber

Alexandria Journal of Agricultural Research, Journal Year: 2024, Volume and Issue: 0(0), P. 0 - 0

Published: July 24, 2024

Petroleum hydrocarbon contamination is one of the main environmental challenges. Bioremediation using fungi (Mycoremediation) best method to overcome this problem. Consequently, current study aimed investigate tolerance four fungal isolates petroleum and estimate level production cellulase Mn peroxidase enzymes involved in oil biodegradation. The were identified as Fusarium solani, Trichoderma harzianum, Aspergillus terreus, flavus by rDNA sequencing ITS1 -5.8 S rRNA-ITS2 region. A test was conducted solid minimal media (MM) containing different concentrations diesel oil, growth rate dose inhibition response percentage (DIRP) values recorded. Treated non-treated Bushnell Haas (BH) broth used ability secret target enzymes. results showed that T. harzianum had highest towards followed A. flavus. Diesel induced gene-overexpression for producer peroxidase. Finally, all isolates, especially can adapt high hydrocarbon. Thus, they be successfully bioremediation

Language: Английский

Biotechnological Potential for Fructooligosaccharides Production and Molecular Characterization of Aspergillus oryzae DIA-ITDAM DOI
Alaín Martínez-Pérez, Felipe de Jesús Mares-Rodríguez, Diana B. Muñiz‐Márquez

et al.

Food Biotechnology, Journal Year: 2025, Volume and Issue: 39(1), P. 94 - 114

Published: Jan. 2, 2025

Language: Английский

Citations

0
Analysis of Whole-Genome as a Novel Strategy for Animal Species Identification DOI Open Access

Yutong Gan,

Guihong Qi,

Lijun Hao

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2955 - 2955

Published: March 3, 2024

Survival crises stalk many animals, especially endangered and rare animals. Accurate species identification plays a pivotal role in animal resource conservation. In this study, we developed an method called Analysis of whole-GEnome (AGE), which identifies by finding species-specific sequences through bioinformatics analysis the whole genome subsequently recognizing these using experimental technologies. To clearly demonstrate AGE method, Cervus nippon, well-known species, closely related elaphus, were set as model without with published genomes, respectively. By analyzing genomes C. nippon obtained next-generation sequencing online databases, built specific sequence databases containing 7,670,140 570,981 sequences, Then, specificities confirmed experimentally Sanger CRISPR-Cas12a system. Moreover, for 11 fresh samples 35 commercially available products, our results complete agreement those other authoritative methods, demonstrating AGE’s precision potential application. Notably, found mixture products successfully identified it. This study broadens horizons sheds light on conserving resources.

Language: Английский

Citations

3
Analysis of Whole-Genome for Identification of Seven Penicillium Species with Significant Economic Value DOI Open Access
Y. H. Huang,

Lianguo Fu,

Yutong Gan

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8172 - 8172

Published: July 26, 2024

The

Language: Английский

Citations

3
The Role of Quantitative Real-Time PCR in the Invasive Pulmonary Aspergillosis Diagnosis: A Retrospective Study DOI Creative Commons
Laura Trovato, Maddalena Calvo,

Concetta Ilenia Palermo

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(2), P. 409 - 409

Published: Feb. 13, 2025

Invasive pulmonary aspergillosis (IPA) reports significant mortality rates among critically ill patients. A prompt microbiological diagnosis is essential to establish a coherent antifungal treatment. Despite its low sensitivity and prolonged turn-around time, culture represents the conventional diagnostic technique. Additionally, galactomannan detection may support process. Ultimate generation methods, such as real-time polymerase chain reaction (Real-Time PCR), integrated procedure improve overall laboratory effectiveness, especially regarding quantitative Aspergillus spp. DNA detection. Herein, we propose retrospective analysis where PCR was performed on respiratory samples belonging patients with or without probable aspergillosis. The study enrolled 62 samples, whose results were compared indexes. clinical general data collected for all qPCR assay reported 100% negative predictive value, while specificity reached 59.2% positive value 76.1%. Moreover, IPA fungal loads higher than 103 in logarithmic scale, non-aspergillosis episodes maximum level of 103. We hypothesized future possibility define specific cut-off distinguishing colonization from infection cases, requiring further investigations speculations about samples.

Language: Английский

Citations

0
Analysis of Whole-Genome for Alternaria Species Identification DOI Creative Commons
Ying Yang,

Yutong Gan,

Wenjie Xu

et al.

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(3), P. 185 - 185

Published: Feb. 26, 2025

The genus Alternaria, functioning as a saprobe, endophyte, and plant pathogen, is widely distributed across various natural human-impacted environments. Leaf spot black diseases, caused by Alternaria species, are the most prevalent diseases within this genus, leading to significant reductions in crop yields substantial economic losses. To facilitate timely detection of species during early stages infection, enable targeted treatments, mitigate associated damages, we employed identification method based on Analysis whole-GEnome (AGE). We downloaded 148 genomes, including 31 from NCBI GenBank database. Through bioinformatics analysis, constructed specific-target sequence library selected representative per species. specific target sequences seven exemplary were subsequently used for validation rapid detection, utilizing Sanger sequencing CRISPR-Cas12a technology, respectively. results demonstrated that our accurately identified Additionally, combining Enzymatic Recombinase Amplification (ERA) with CRISPR-Cas12a, achieved precise genomic DNA samples, limit low 0.01 ng/µL 30 min. Therefore, AGE proves be highly robust efficient offering broad potential applications.

Language: Английский

Citations

0
MALDI TOF-MS for microbial identification and diagnosis DOI

Jayalaxmi Dash,

Ankita Naykodi,

Nirmal Kumar Mohakud

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 43 - 57

Published: Jan. 1, 2024

Citations

0
Biodegradation of petroleum hydrocarbon and related molecular microbiology analysis of some fungal species DOI Creative Commons
Huda M. Shakam, Mahmoud A. Gaber

Alexandria Journal of Agricultural Research, Journal Year: 2024, Volume and Issue: 0(0), P. 0 - 0

Published: July 24, 2024

Petroleum hydrocarbon contamination is one of the main environmental challenges. Bioremediation using fungi (Mycoremediation) best method to overcome this problem. Consequently, current study aimed investigate tolerance four fungal isolates petroleum and estimate level production cellulase Mn peroxidase enzymes involved in oil biodegradation. The were identified as Fusarium solani, Trichoderma harzianum, Aspergillus terreus, flavus by rDNA sequencing ITS1 -5.8 S rRNA-ITS2 region. A test was conducted solid minimal media (MM) containing different concentrations diesel oil, growth rate dose inhibition response percentage (DIRP) values recorded. Treated non-treated Bushnell Haas (BH) broth used ability secret target enzymes. results showed that T. harzianum had highest towards followed A. flavus. Diesel induced gene-overexpression for producer peroxidase. Finally, all isolates, especially can adapt high hydrocarbon. Thus, they be successfully bioremediation

Language: Английский

Citations

0