Composition and diversity of microbial endophytes in roots, stems, and leaves of Angelica sinensis in three main planting areas

South African Journal of Botany, Journal Year: 2025, Volume and Issue: 180, P. 305 - 314

Published: March 19, 2025

Language: Английский

Climate change impacts on tropical sponges and associated microbial communities

Published: April 28, 2024

<p><strong>Climate change is causing rapid changes in reef structure, biodiversity, and function as a response to ocean warming acidification. The negative impacts of climate on corals are well-documented, but most sponges predicted tolerate conditions projected for 2100, making them viable option stable alternative states. Sponges maintain an intimate relationship with microbial communities, whose general stability given sponge species makes suitable indicators host responses stressful environmental such overall aim this thesis examine the their associated focus community composition abundance protein expression.</strong></p><p>The first data chapter examines results from reciprocal transplantation coral Coelocarteria singaporensis Stylissa cf. flabelliformis between control site adjacent CO2 vent Papua New Guinea explore how microbiome responds acidification situ. Microbial communities C. singaporensis, which differed initially sites, did not shift towards characteristic or microbiomes. S. flabelliformis, were respond specifically collectively exhibited significant over time, relative increase Thaumarchaeota decrease Proteobacteria all treatment groups. lack upon suggests that flexibility, at least adult life-history stage, does necessarily underpin survival under acidification.</p><p>The second compares symbiotic tropical after eight-week exposure nine different treatments three temperatures (28.5 °C, 30 31.5 °C) pH levels (8.1, 7.8, 7.6) based ambient two IPCC predictions future (RCP6.0 RCP8.5). Bacterial significantly temperature treatments, pH, highest showing increased abundances Bacteroidia Clostridia (commonly disease thermal stress), decreased Nitrospira (associated nitrogen cycling). Similarly, archaeal across temperature, Nitrososphaeria ammonia oxidation). Symbiotic eukaryotes compared using reference databases only 8.1 difference also highlighted requirement more thorough databases. Overall, there was clear groups, indicating stress potential hindrance nutrient cycling.</p><p>The third aims determine impact spatial arrangement sponge-associated microbes droplet digital PCR (ddPCR) fluorescence situ hybridization (FISH). Total bacterial unexpectedly high replicates notable algal growth tissue necrosis. Archaea decreasing increasing no due pH. FISH largely unsuccessful, primarily autofluorescence very low concentration archaea (as shown ddPCR). Regardless, shifts reduced health show signs functional implications.</p><p>The fourth uses proteomic analysis expression High-temperature proteins heat shock oxidative stress, actin-related processes, smooth muscle contraction comparison low-temperature treatments. high- low-pH resulted annotated biological processes. Oxidation higher 7.6 while lower 7.6. important functions reduction indicate flabelliformis.</p><p>Overall, expected be resilient than calcifying organisms corals. Although produced little holobiont, exhibits threshold approximately °C. At implications roles sponges.</p>

Language: Английский

Characterizing the rhythmic oscillations of gut bacterial and fungal communities and their rhythmic interactions in male cynomolgus monkeys

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 25, 2024

ABSTRACT The circadian oscillation of gut microbiota plays vital roles in the normal physiology and health host. Although diurnal intestinal bacteria has been extensively studied, little relevant work done on fungi. Besides, rhythmic correlations between bacterial fungal microbes are also scarcely reported. Here, we investigated oscillations communities male cynomolgus monkeys by performing 16S rRNA ITS amplicon sequencing. As for genera, found that relative abundance Prevotella , norank_f_Eubacterium_coprostanoligenes_group, Peptococcus underwent significant changes at ZT12 (19:00) exhibited obvious oscillations. Consequently, most functions varied were positively correlated with genera norank_f_Eubacterium_coprostanoligenes_group . Among Aspergillus Talaromyces decreased ZT18 (1:00) showed slight function, undefined saprotroph was genus Notably, characterized fungi every 6 h over course a day interacted closely, bacteria–fungi interactions occurring ZT12. Our study contributed to more comprehensive understanding patterns uncovered their during cycle. IMPORTANCE can impact activity disease susceptibility Until now, studies focused microbes, ignoring other components such as (mycobiota). only few have addressed analyzed Apart from identifying rhythmically oscillated conducted correlation analysis these two microbial close rhythmically, Thus, our not but interactions.

Language: Английский