Effect of Genotype and Age on a Defined Microbiota in Gnotobiotic SCID Piglets DOI Creative Commons

Katherine Marie Widmer,

Faith M. Rahic-Seggerman,

A Forster

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 7, 2024

Severe combined immunodeficient (SCID) individuals lack functional T and B lymphocytes, leading to a deficient adaptive immune system. SCID pigs are unique large animal biomedical model as they possess many similarities humans, allowing for the collection of translatable data in regenerative medicine, cancer, other research topics. While studies suggest early gut microbiota development is necessary developing intestinal barrier system, these animals often cesarian section derived, leaving them uncolonized normal microflora. The hypothesis was that an increase complexity inoculum will allow more stability composition piglets. This tested across multiple litters piglets with three different defined consortium (2-strain, 6-strain, 7-strain). All received their designated by oral gavage immediately after birth again 24 hours later. There no effect genotype on microbiota, but there significant due piglet age. Additionally, all consortia were deemed safe use piglets, 7-strain most stable over time. Based results, be added pig husbandry protocol, reproducible model.

Language: Английский

Development of an innovative intravaginal model of probiotic inoculation in dairy ovine flocks: positive effects on the vaginal microbiota, vaginitis and fertility DOI Creative Commons
Marion Toquet, Jesús Gomis, E. Jiménez-Trigos

et al.

animal, Journal Year: 2025, Volume and Issue: 19(2), P. 101417 - 101417

Published: Jan. 5, 2025

In vitro studies have reported the antimicrobial effect of an inoculum (L2) made from commercial probiotic strains Lactobacillus spp. (Lactobacillus (L.) crispatus, L. brevis and gasseri) against some ruminants' pathogens. However, their possible beneficial effects on controlling vaginosis caused by use intravaginal sponges for oestrus synchronisation not been evaluated in vivo models. Therefore, purpose this study was to perform double inoculation same non-lyophilised (non-freeze-dried) L2 inoculum, 120 ewes four dairy ovine flocks using straws similar those used artificial insemination. Globally, entire population, fertility percentage (65%) inoculated groups increased 10% compared control (55%). Specifically, increases percentages pregnant sheep 1 4 (10.7 5.7% respectively; P > 0.05) flock 3 (27.3%; < were observed ewes. Overall, administration day sponge insertion decreased number with vaginitis 11.13% at time removal (T1). first flock, positively modulated microbiota richness presence Lactobacillales accompanied a reduction Mycoplasma Genera such as Fusobacterium, Streptobacillus, Campylobacter or Trueperella linked T1. belonging Lactobacillales, including genera previously be pregnancy, significantly pregnancy diagnosis but The results suggest that probiotics via strategy modulate vaginal inflammation, derived sponges, is also associated improved fertility. This novel approach could improve animal welfare oestrus-synchronisation protocols represents alternative prophylactic antibiotics.

Language: Английский

Citations

1
Combined analysis of 16S rRNA gene sequencing data reveals core vaginal bacteria across livestock species DOI Creative Commons
Lucille C. Jonas, Curtis R. Youngs, Stephan Schmitz‐Esser

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 10, 2025

Investigating the livestock vaginal microbiota is of increasing interest due to its relationship with animal reproductive performance. Recent publications have uncovered a high degree variability microbiota, making it difficult focus functional research on individual microorganisms. To address this variability, we conducted combined analysis publicly available 16S rRNA gene amplicon sequencing datasets reveal core in cattle, sheep, and pigs. The goal was identify bacterial genera that were shared despite diverse overall sample population. A total 2,911 samples (715 964 1,232 pigs) from 29 different used analysis. Beta diversity revealed structural differences between species. Compositionally, most abundant phyla Bacillota , Pseudomonadota Bacteroidota. At genus level, an unclassified Pasteurellaceae genus, Ureaplasma Streptococcus abundant. Across species, compositional observed. cattle sheep contained higher abundance Histophilus whereas pig more Fusobacterium Parvimonas than other samples. Among 120 OTUs 82 present 70% same threshold, had 40 63 genera, while 22 50 genera. There 19 overlapping across three largely species-specific, although there eight OTUs. These included (OTU 21), Clostridium sensu stricto 1 18), Corynebacterium 6), which also some members microbiota. better understanding required for future studies aimed at elucidation significance microbes respect efficiency. identified will help guide mechanisms/pathways through organisms enhance or impede

Language: Английский

Citations

0
Metabarcoding analysis of the microbiota in flocks naturally infected by Coxiella burnetii: First description of the global microbiota in domestic small ruminants DOI Creative Commons
Raquel Toledo-Perona, Ángel Gómez‐Martín, A. Contreras

et al.

One Health, Journal Year: 2025, Volume and Issue: 20, P. 100996 - 100996

Published: Feb. 19, 2025

Language: Английский

Citations

0
Use of a defined microbiota for maintenance of gnotobiotic SCID piglets DOI Open Access

Katherine Marie Widmer

Published: Jan. 1, 2024

Severe combined immunodeficient (SCID) individuals lack functional T and B lymphocytes, leading to a deficient adaptive immune system. SCID pigs are unique, large animal, biomedical model as they possess many similarities humans. This allows for the collection of translatable data in regenerative medicine, cancer, other research topics. While studies suggest early gut microbiota development is necessary molding intestinal barrier system, these highly disease-susceptible animals often cesarian section derived, leaving them uncolonized microflora. My hypothesis was that an increase complexity inoculum will allow more stability composition piglets. tested across multiple litters piglets with three different defined consortia varied complexity. I measured effect genotype piglet age on diversity well inoculated over time. Additionally, wanted determine if use safe The bacteria selected inoculation did not cause observable harm piglets, most complex stable microbiome. Giving reproducible model, standardized can be created collaborators, work this model.

Language: Английский

Citations

0
Longitudinal study of the bovine cervico-vaginal bacterial microbiota throughout pregnancy using 16S ribosomal RNA gene sequences DOI Creative Commons
Lucía Calleros,

Maila Barcellos,

Sofía Grecco

et al.

Infection Genetics and Evolution, Journal Year: 2024, Volume and Issue: 124, P. 105657 - 105657

Published: Aug. 19, 2024

The microbiota composition of the bovine female reproductive tract influences efficiency, susceptibility to genital pathogens, and health newborn calves. However, knowledge about cervico-vaginal during gestation is scarce. Therefore, present study aimed analyze taxonomic profile throughout pregnancy after calving using high-throughput sequencing a fragment 16S ribosomal RNA gene. Healthy nulliparous Holstein heifers (n = 13) with similar age body conditional score were selected collect samples from area sterile swab at 5 timepoints. We sequenced V1-V2 region gene analyzed data DADA2, phyloseq vegan R Studio packages. No differences observed in alpha beta diversity across sampling points, accounting for stability pregnancy. most abundant phyla are Firmicutes, Bacteroidota, Proteobacteria Actinobacteria, as main taxa all five points. Also, several least can be change time. Our comprehensive bacterial period contributes dynamics on serve baseline future research development potential therapeutic interventions.

Language: Английский

Citations

0
Effect of Genotype and Age on a Defined Microbiota in Gnotobiotic SCID Piglets DOI Creative Commons

Katherine Marie Widmer,

Faith M. Rahic-Seggerman,

A Forster

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 7, 2024

Severe combined immunodeficient (SCID) individuals lack functional T and B lymphocytes, leading to a deficient adaptive immune system. SCID pigs are unique large animal biomedical model as they possess many similarities humans, allowing for the collection of translatable data in regenerative medicine, cancer, other research topics. While studies suggest early gut microbiota development is necessary developing intestinal barrier system, these animals often cesarian section derived, leaving them uncolonized normal microflora. The hypothesis was that an increase complexity inoculum will allow more stability composition piglets. This tested across multiple litters piglets with three different defined consortium (2-strain, 6-strain, 7-strain). All received their designated by oral gavage immediately after birth again 24 hours later. There no effect genotype on microbiota, but there significant due piglet age. Additionally, all consortia were deemed safe use piglets, 7-strain most stable over time. Based results, be added pig husbandry protocol, reproducible model.

Language: Английский

Citations

0