Identification of Pseudomonas protegens and Bacillus subtilis Antimicrobials for Mitigation of Fuel Biocontamination

Biomolecules, Journal Year: 2025, Volume and Issue: 15(2), P. 227 - 227

Published: Feb. 4, 2025

Hydrocarbon fuel biofouling and biocorrosion require expensive cleanup of aviation infrastructures unless appropriate sustainment measures are applied. The identification novel biological control agents offers promising alternatives to the current chemical biocides used in sustainment. In this study, 496 microbial isolates from our in-house repository were screened identify new endogenously produced antimicrobial compounds. Using agar plug screening, liquid culture growth testing, Jet A assays, two fuel-isolate strains Pseudomonas protegens #133, Bacillus subtilis #232 demonstrated biocontrol activity against bacteria, yeast, filamentous fungi. Liquid chromatography-quadrupole time flight tandem mass spectrometry (LC-QTOF-MS/MS) filtrate identified several common lipopeptide antimicrobials including gageostatin C, gageopeptin B, miscellaneous macrolactins. contrast, LC-QTOF-MS/MS siderophore pyochelin as one predominant compounds #133 with previously effect. consortium testing flow-cytometry live/dead cell mechanism determination action Gram-positive bacteria. study concludes that secreted by have bactericidal effects Gordonia sp. cause death through bacterial lysis membrane damage potential applications biocidal treatment hydrocarbon-based fuels.

Language: Английский

Diversity and composition of soil microbial communities in the rhizospheres of late blight-resistant tomatoes after Phytophthora infestans inoculation

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 7, 2025

Late blight caused by the oomycete Phytophthora infestans poses a severe threat to global tomato ( Solanum lycopersicum L.) production. While genetic resistance forms cornerstone of disease control, mechanisms underlying cultivar-specific resistance, particularly their interactions with rhizosphere microbiomes, remain poorly understood. To elucidate cultivar late and screen out antagonistic microorganisms against P . , we investigated microbial compositions in rhizospheres cultivars different blight-resistance levels under both natural P. -inoculated conditions. Considerable differences soil diversity composition were found between blight-resistant -susceptible cultivars. Under conditions, resistant exhibited higher bacterial lower fungal than that susceptible cultivar. Additionally, after inoculation, showed enrichment potential effects rhizospheres. Among them, genera, such as Pseudomonas Azospirillum Acidovorax including Phoma Arthrobotrys Pseudallescheria Pseudolabrys enriched In contrast, Flavobacterium Burkholderia-Caballeronia-Paraburkholderia Trichoderma genus blight-susceptible Simultaneously, pathogenic microorganisms, Neocosmospora Plectosphaerella was also detected Moreover, no occurred inoculation. These findings suggest these traits serve effective defense pathogen invasion Overall, this study provides comprehensive analysis community structures conditions response following identified. The offer valuable insights for management tomatoes contribute development sustainable agricultural practices.

Language: Английский

Use of sheep myeloid antimicrobial peptide (SMAP-18) and siderophore Pyochelin for mitigation of aerospace fuel-degrading microbes

International Biodeterioration & Biodegradation, Journal Year: 2024, Volume and Issue: 196, P. 105943 - 105943

Published: Oct. 30, 2024

Language: Английский