Clock genes regulate sex pheromone production and male mating ability in Bactrocera dorsalis

Insect Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

Many animals display physiological and behavioral activities limited to specific times of the day. Certain insects exhibit clear daily rhythms in their mating that are regulated by an internal biological clock. However, genetic mechanisms underlying this regulation remain largely unexplored. Mating fruit fly Bactrocera dorsalis exhibits a rhythm is dependent on sex pheromones produced male rectum. We used transcriptome sequencing clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR-associated nuclease 9 techniques understand whether rhythmicity B. pheromone production rectum clock genes. The results showed males rhythmic (low during day high at night) influenced Knockout genes cryptochrome 1 (cry1) timeless (tim) reduced significantly impaired ability males. In addition, quantitative polymerase chain reaction from 5 different tissues cry1 was highly expressed head, whereas tim both head (a key site for production). Transcriptome analysis confirmed (head) (head rectum) expressions consistent with rhythmicity. These suggest may be related central neuron (like suprachiasmatic nucleus), expression indicates potential presence peripheral oscillators. Our study reveals new targets ideas improved control fly.

Language: Английский

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Effects of microbial biocontrol agents on tea plantation microecology and tea plant metabolism: a review

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 20, 2025

The quality of fresh tea leaves is crucial to the final product, and maintaining microbial stability in plantations essential for optimal plant growth. Unique communities play a critical role shaping flavor enhancing resilience against biotic stressors. Tea production frequently challenged by pests diseases, which can compromise both yield quality. While stress generally has detrimental effects on plants, it also activates defense metabolic pathways, leading shifts communities. Microbial biocontrol agents (MBCAs), including entomopathogenic antagonistic microorganisms, present promising alternative synthetic pesticides mitigating these stresses. In addition controlling MBCAs influence composition communities, potentially health resilience. However, despite significant advances laboratory research, field-level impacts microecology remain insufficiently explored. This review provides insights into interactions among insects, offering strategies improve pest disease management plantations.

Language: Английский

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Insecticidal Action of Local Isolates of Entomopathogenic Fungi Against Bactrocera oleae Pupae

Biology, Journal Year: 2024, Volume and Issue: 14(1), P. 5 - 5

Published: Dec. 24, 2024

In this work, the ability of certain entomopathogenic fungi to control olive fruit fly Bactrocera oleae (Diptera: Tephritidae), which significantly affects cultivation, was assessed. First, that often contribute reducing pests, as well B. oleae, were sought out. Puparia collected from oil mills, and soil samples various grove regions Greece. These soils used substrate in effect on pupae studied. addition, same treatments studied non-soil conditions. The results show known are able, their natural environment, kill a significant extent. survival time for adults (days) lower presence Beauveria bassiana (Hypocreales: Cordycipitaceae), at 13.20 (5.56 ± 0.42) (6.76 0.46) conditions, compared (9.83 083) (9.90 0.72) This is encouraging Mediterranean where while quality high demand.

Language: Английский

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Whole-Genome Sequencing of the Entomopathogenic Fungus Fusarium solani KMZW-1 and Its Efficacy Against Bactrocera dorsalis

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(10), P. 11593 - 11612

Published: Oct. 17, 2024

Fusarium solani KMZW-1 is recognized for its potential as a biocontrol agent against agricultural and forestry pests, particularly due to compatibility with integrated pest management (IPM) strategies. This study aimed investigate the complete genome of F. assess pathogenicity Bactrocera dorsalis. Whole-genome sequencing revealed size 47,239,278 bp, comprising 27 contigs, GC content 51.16% fungus identified KMZW-1. The completeness was assessed 97.93% using BUSCO analysis, DFVF sequence identifier 0G092560.1, AntiSMASH analysis 35 gene clusters associated secondary metabolite biosynthesis, providing insights into genetic basis pathogenic mechanisms potential. Comparative genomic found 269 unique genes KMZW-1, collinearity exhibited high degree synteny solani-melongenae. concentrations ranging from 1 × 104 1011 conidia/mL. Higher (1 1010 conidia/mL) resulted in significantly increased cumulative mortality rates B. dorsalis adults compared control group. Notably, higher male than females. Probit yielded LC50 (50% lethal concentration) values 5.662 female 4.486 adults. In summary, solani, exhibits strong insecticidal activity shows IPM These findings provide robust evidence supporting use managing populations.

Language: Английский

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Comparative Population Biology and Related Gene Expression in the Beta-Cypermethrin-Resistant Strains of Bactrocera dorsalis (Hendel)

Insects, Journal Year: 2024, Volume and Issue: 15(8), P. 569 - 569

Published: July 26, 2024

Diptera and Lepidoptera species have the highest levels of insecticide resistance, mechanism drug resistance has been studied in detoxification metabolism genes such as P450, GST, EST, ABC. Since Bactrocera dorsalis are resistant to a variety chemicals, pattern investigated from aspects genes, enzymes, intestinal symbiotic bacteria, synergists world. In this study, 51 149 were annotated Suppression Subtractive Hybridization (SSH) library, 12 candidate related beta-cypermethrin screened quantitatively expressed library. Two found be upregulated egg stage, three larval one gene pupal five adult four midgut malacca ducts midgut. The expression cyp6g1, cyp6a22, GST-Epsilon9, Trypsin-4 was strains, with most obvious upregulation occurring Malpighian tubules. These results provide new insights into study pesticide quarantine insects.

Language: Английский

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