Occurrence, antimicrobial resistance and genomic features of Klebsiella pneumoniae from broiler chicken in Faisalabad, Pakistan DOI Creative Commons
Muhammad Moman Khan,

Muhammad Ahmed Mushtaq,

Nayyar Abbas

et al.

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: Aug. 19, 2024

Introduction The dissemination of antimicrobial resistance (AMR) in critical priority pathogens is a significant threat. Non-clinical reservoirs AMR, such as agriculture and food production facilities, may contribute to the transmission clinically relevant multidrug-resistant (MDR) Klebsiella pneumoniae . There currently very limited knowledge regarding population structure genomic diversity K. poultry Pakistan. Methods We explored healthy broilers commercial farm from Faisalabad, Pakistan, identified six strains 100 broiler birds. characterized strains, determining clonality, virulence genes using next generation sequencing. Results evaluation susceptibility revealed that all were MDR. Genomic analysis showed 3/6 belonged ST152, harbouring acquired aminoglycosides [ aadA2 , aph(4′)-Ia ], β-lactams ( bla SHV-187 LAP2 ), fosfomycin fosA6 tetracycline tetA trimethoprim dfrA12 quinolone qnrS1 sulphonamides sul2 ) phenicol floR ). All harboured efflux pump oqxA oqxB emrR kpnG kpnH kpnF baeR mtdB mtdC encoded identical profiles possessing genes, i.e., ureA iutA entB allS fimH mrkD Phylogenomic dominant sequence type (ST152) present our dataset with publicly available genomes isolates clustered mainly human sources could pose potential threat safety public health. Discussion combination these findings use data would allow better understanding selective pressures be driving spread AMR. This first report MDR isolated hens finding suggests routine surveillance WHO settings beneficial development effective control strategies reduce

Language: Английский

Prevalence and Antimicrobial Resistance of Extended Spectrum Beta-Lactamase (ESBL) Producing Klebsiella spp. in Poultry Meat DOI Creative Commons
Fatema Yeasmin Tanni, Md. Shahidur Rahman Chowdhury, Hemayet Hossain

et al.

Heliyon, Journal Year: 2025, Volume and Issue: 11(1), P. e41748 - e41748

Published: Jan. 1, 2025

Klebsiella spp. present in the food chain have been of much interest during last few decades due to their implication development antimicrobial resistance. We determined prevalence (Klebsiella pneumoniae and oxytoca) retail poultry meat, along with resistance profile against antimicrobials. For detection a total 285 raw meat samples retailed were collected cultured. All positive cultures screened for by PCR confirm identity. Their susceptibility pattern was performed using disk diffusion technique, whereas ESBL-coding genes detected multiplex technique. Out samples, 139 (48.77 %) had While out identified isolates, 99 (34.74 isolates K. pneumoniae, 13 (4.56 oxytoca. Phenotypically, most strains fully (100 resistant ampicillin, amoxicillin, cefuroxime, ceftazidime, nalidixic acid. Of β-Lactamase that through mPCR, bla SHV dominant gene among oxytoca percentages 86.87 % 76.92 %, respectively. Besides, p n eumoniae harbored 95.96 (95 CI: 89.98-98.89) tetA gene, only 92.31 63.97-99.81) gene. Most pneumonia e found harbor streptomycin-resistant strA. These results highlighted significant carriage multidrug-resistant (MDR) which insinuates there is need strategic plan place curb dissemination these multi drug pathogens chain.

Language: Английский

Citations

1
Emerging biofilm formation and disinfectant susceptibility of ESBL-producing Klebsiella pneumoniae DOI Creative Commons
Hanan S. Khalefa, Amany A. Arafa, Dalia Hamza

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 10, 2025

Abstract Klebsiella pneumoniae is an opportunistic pathogen responsible for various infections in humans and animals. It known its resistance to multiple antibiotics, particularly through the production of Extended-Spectrum Beta-Lactamases (ESBLs), ability form biofilms that further complicate treatment. This study aimed isolate identify K. from animal environmental samples assess commercial disinfectants’ effectiveness against isolates exhibiting ESBL-mediated biofilm-forming poultry equine farms Giza Governorate, Egypt. A total 320 samples, including nasal swabs (n = 60) broiler chickens 90), 140), human hand 30), were collected. was isolated using lactose broth enrichment MacConkey agar, with molecular confirmation via PCR targeting gyrA magA genes. also identified ESBL genes ( bla TEM , SHV CTX-M OXA-1 ) biofilm luxS, Uge, mrkD ). Antimicrobial susceptibility assessed, efficacy five disinfectants evaluated by measuring inhibition zones. (13.3%), (8.3%), wild birds (15%), water (10%), feed (2%), (6.6%). detected majority isolates, significant phenotypic antibiotics. The containing peracetic acid hydrogen peroxide most effective, producing largest zones, while based on sodium hypochlorite isopropanol showed lower efficacy. Statistical analysis revealed differences across sample origins (P < 0.05). presence sources, along high prevalence biofilm-associated virulence genes, underscores zoonotic potential this pathogen. demonstrated are highly effective ESBL-producing . Implementing appropriate biosecurity measures, use disinfectants, essential controlling spread resistant pathogens farm environments.

Language: Английский

Citations

0
A genome-wide One Health study of Klebsiella pneumoniae in Norway reveals overlapping populations but few recent transmission events across reservoirs DOI Creative Commons
Marit Andrea Klokkhammer Hetland, Mia A. Winkler, Håkon Kaspersen

et al.

Genome Medicine, Journal Year: 2025, Volume and Issue: 17(1)

Published: April 28, 2025

Language: Английский

Citations

0
Genomic Characterization of Multidrug-Resistant Enterobacteriaceae Clinical Isolates from Southern Thailand Hospitals: Unraveling Antimicrobial Resistance and Virulence Mechanisms DOI Creative Commons
Thunchanok Yaikhan,

Sirikan Suwannasin,

Kamonnut Singkhamanan

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(6), P. 531 - 531

Published: June 6, 2024

The emergence and spread of antimicrobial resistance (AMR) among Enterobacteriaceae pose significant threats to global public health. In this study, we conducted a short-term surveillance effort in Southern Thailand hospitals characterize the genomic diversity, AMR profiles, virulence factors strains. We identified 241 carbapenem-resistant Enterobacteriaceae, which 12 were selected for whole-genome sequencing (WGS) genome analysis. strains included Proteus mirabilis, Serratia nevei, Klebsiella variicola, aerogenes, indica, grimontii, Phytobacter ursingii, palmae, Kosakonia spp., Citrobacter freundii. exhibited high levels multidrug resistance, including carbapenem antibiotics. Whole-genome revealed diverse array genes (ARGs), with carrying ß-lactamase, efflux pumps, other antibiotic classes. Additionally, stress response, metal tolerance, virulence-associated identified, highlighting adaptability pathogenic potential these A plasmid analysis several replicons, IncA/C2, IncFIB(K), Col440I, as well plasmids identical those found globally, indicating horizontal gene transfer ARGs. Importantly, study also novel species spp. PSU27, adding understanding genetic diversity mechanisms Thailand. results reported highlight critical importance implementing effective management programs developing innovative treatment approaches urgently tackle AMR.

Language: Английский

Citations

1
Review: Review of antimicrobial resistance of Klebsiella pneumoniae isolated from poultry, cattle and pigs DOI Creative Commons
Barbara Kot, Małgorzata Witeska

animal, Journal Year: 2024, Volume and Issue: 18(11), P. 101345 - 101345

Published: Sept. 26, 2024

Language: Английский

Citations

1
Occurrence, antimicrobial resistance and genomic features of Klebsiella pneumoniae from broiler chicken in Faisalabad, Pakistan DOI Creative Commons
Muhammad Moman Khan,

Muhammad Ahmed Mushtaq,

Nayyar Abbas

et al.

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: Aug. 19, 2024

Introduction The dissemination of antimicrobial resistance (AMR) in critical priority pathogens is a significant threat. Non-clinical reservoirs AMR, such as agriculture and food production facilities, may contribute to the transmission clinically relevant multidrug-resistant (MDR) Klebsiella pneumoniae . There currently very limited knowledge regarding population structure genomic diversity K. poultry Pakistan. Methods We explored healthy broilers commercial farm from Faisalabad, Pakistan, identified six strains 100 broiler birds. characterized strains, determining clonality, virulence genes using next generation sequencing. Results evaluation susceptibility revealed that all were MDR. Genomic analysis showed 3/6 belonged ST152, harbouring acquired aminoglycosides [ aadA2 , aph(4′)-Ia ], β-lactams ( bla SHV-187 LAP2 ), fosfomycin fosA6 tetracycline tetA trimethoprim dfrA12 quinolone qnrS1 sulphonamides sul2 ) phenicol floR ). All harboured efflux pump oqxA oqxB emrR kpnG kpnH kpnF baeR mtdB mtdC encoded identical profiles possessing genes, i.e., ureA iutA entB allS fimH mrkD Phylogenomic dominant sequence type (ST152) present our dataset with publicly available genomes isolates clustered mainly human sources could pose potential threat safety public health. Discussion combination these findings use data would allow better understanding selective pressures be driving spread AMR. This first report MDR isolated hens finding suggests routine surveillance WHO settings beneficial development effective control strategies reduce

Language: Английский

Citations

0