Surface-mediated Bacteriophage Defense Incurs Fitness Tradeoffs for Interbacterial Antagonism DOI Creative Commons
Chia-En Tsai, Fengqi Wang,

Chih-Wen Yang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 14, 2024

ABSTRACT Bacteria in polymicrobial habitats are constantly exposed to biotic threats from bacteriophages, antagonistic bacteria, and predatory eukaryotes. These interactions play crucial roles shaping the evolution physiology of bacteria. To survive, bacteria have evolved mechanisms protect themselves such attacks, but fitness costs resisting one threat rendering susceptible others remain unappreciated. Here, we examined consequences bacteriophage resistance Salmonella enterica , revealing that phage-resistant variants exhibited significant loss upon co-culture with competitor strains display varying degrees lipopolysaccharide (LPS) deficiency increased susceptibility contact-dependent interbacterial antagonism, as type VI secretion system (T6SS). Utilizing mutational analyses atomic force microscopy, show long-modal length O-antigen LPS serves a protective barrier against T6SS-mediated intoxication. Notably, this competitive disadvantage can also be triggered independently by phages possessing LPS-targeting endoglycosidase their tail spike proteins, which actively cleave infection. Our findings reveal two distinct phage-mediated modifications modulate competition, shedding light on dynamic microbial interplay within mixed populations.

Language: Английский

Whole-genome sequencing of Acinetobacter baumannii clinical isolates from a tertiary hospital in Terengganu, Malaysia (2011–2020), revealed the predominance of the Global Clone 2 lineage DOI Creative Commons

Nurul Saidah Din,

Farahiyah Mohd. Rani,

Ahmed Ghazi Alattraqchi

et al.

Microbial Genomics, Journal Year: 2025, Volume and Issue: 11(2)

Published: Feb. 5, 2025

Carbapenem-resistant Acinetobacter baumannii is recognized by the World Health Organization (WHO) as one of top priority pathogens. Despite its public health importance, genomic data clinical isolates from Malaysia remain scarce. In this study, whole-genome sequencing was performed on 126 A . collected main tertiary hospital in state Terengganu, Malaysia, over a 10-year period (2011–2020). Antimicrobial susceptibilities determined for 20 antibiotics belonging to 8 classes showed that 77.0% ( n =97/126) were categorized multidrug resistant (MDR), with all MDR being carbapenem resistant. Multilocus sequence typing analysis Terengganu A. into 34 Pasteur and 44 Oxford types (STs), ST2 Global Clone 2 lineage identified dominant ST =76/126; 60.3%). The could be subdivided six STs majority ST195 =35) ST208 =17). Various antimicrobial resistance genes bla OXA-23 -encoded carbapenemase predominant acquired gene =90/126; 71.4%). Plasmid-encoded rep nearly =122/126; 96.8%) Rep_3 family =121). virulence factors identified, highlighting pathogenic nature bacterium. Only 14/126 (11.1%) positive carriage CRISPR-Cas arrays none prevalent harbouring them. This study provided snapshot obtained single healthcare centre predominance closely related lineage, indicating entrenchment clone hospital.

Language: Английский

Citations

1
Surface-mediated bacteriophage defense incurs fitness tradeoffs for interbacterial antagonism DOI Creative Commons
Chia-En Tsai,

F. Y. Wang,

Chih‐Wen Yang

et al.

The EMBO Journal, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

Language: Английский

Citations

0
Environmental and clinical impacts of antibiotics’ sub-minimum inhibitory concentrations on the development of resistance in acinetobacter baumannii DOI
Bipin Yadav,

Dilip D Karad,

Kiran R. Kharat

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 979, P. 179521 - 179521

Published: April 27, 2025

Language: Английский

Citations

0
Comprehensive Approaches to Combatting Acinetobacter baumannii Biofilms: From Biofilm Structure to Phage-Based Therapies DOI Creative Commons

Ilona Grygiel,

Olaf Bajrak,

Michał Wójcicki

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(11), P. 1064 - 1064

Published: Nov. 8, 2024

Acinetobacter baumannii—a multidrug-resistant (MDR) pathogen that causes, for example, skin and soft tissue wounds; urinary tract infections; pneumonia; bacteremia; endocarditis, particularly due to its ability form robust biofilms—poses a significant challenge in clinical settings. This structure protects the bacteria from immune responses antibiotic treatments, making infections difficult eradicate. Given rise resistance, alternative therapeutic approaches are urgently needed. Bacteriophage-based strategies have emerged as promising solution combating A. baumannii biofilms. Phages, which viruses specifically infect bacteria, offer targeted effective means of disrupting biofilm lysing bacterial cells. review explores current advancements bacteriophage therapy, focusing on potential treating biofilm-related infections. We described mechanisms by phages interact with biofilms, challenges phage therapy implementation, being developed enhance efficacy (phage cocktails, engineered phages, combination therapies antibiotics). Understanding role bacteriophages both disruption inhibition forming could pave way innovative treatments MDR well prevention their development.

Language: Английский

Citations

3
The Arms Race Between Actinobacillus pleuropneumoniae and Its Genetic Environment: A Comprehensive Analysis of Its Defensome and Mobile Genetic Elements DOI Creative Commons
Giarlã Cunha da Silva, Ciro César Rossi

Molecular Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: May 3, 2025

ABSTRACT Actinobacillus pleuropneumoniae is the causative agent of pleuropneumonia in swine, a highly contagious and economically significant disease. The genetic variability A. complicates disease control efforts, as it enables rapid adaptation to various stressors, including antimicrobial treatments. To better understand molecular mechanisms underlying this adaptability, we investigated role bacterial defensome its relationship with mobile elements (MGEs), such prophages, plasmids, integrative conjugative (ICEs). Using bioinformatic tools, identified diverse rich , an average 16 different defense systems per strain. We found that CRISPR‐Cas systems, along other mechanisms, are actively involved restricting entry foreign material, playing crucial adaptation. Additionally, characterized several novel prophages examined their distribution across strains, revealing potential contribution bacterium's evolutionary success. Our findings underscore complex interplay between MGEs, shedding light on how maintains diversity while also safeguarding itself against external threats. These insights provide understanding factors influence pathogen's adaptability highlight avenues for more effective strategies.

Language: Английский

Citations

0
Surface-mediated Bacteriophage Defense Incurs Fitness Tradeoffs for Interbacterial Antagonism DOI Creative Commons
Chia-En Tsai, Fengqi Wang,

Chih-Wen Yang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 14, 2024

ABSTRACT Bacteria in polymicrobial habitats are constantly exposed to biotic threats from bacteriophages, antagonistic bacteria, and predatory eukaryotes. These interactions play crucial roles shaping the evolution physiology of bacteria. To survive, bacteria have evolved mechanisms protect themselves such attacks, but fitness costs resisting one threat rendering susceptible others remain unappreciated. Here, we examined consequences bacteriophage resistance Salmonella enterica , revealing that phage-resistant variants exhibited significant loss upon co-culture with competitor strains display varying degrees lipopolysaccharide (LPS) deficiency increased susceptibility contact-dependent interbacterial antagonism, as type VI secretion system (T6SS). Utilizing mutational analyses atomic force microscopy, show long-modal length O-antigen LPS serves a protective barrier against T6SS-mediated intoxication. Notably, this competitive disadvantage can also be triggered independently by phages possessing LPS-targeting endoglycosidase their tail spike proteins, which actively cleave infection. Our findings reveal two distinct phage-mediated modifications modulate competition, shedding light on dynamic microbial interplay within mixed populations.

Language: Английский

Citations

0