Probiotic characteristics and whole genome sequencing of Pediococcus pentosaceus SNF15 and its protective effect on mice diarrhea induced by Escherichia coli K99 DOI Creative Commons
Yu‐Ching Su,

Mingque Feng,

Jun Tong

et al.

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: March 13, 2025

Escherichia coli ( E. col iK99) is one of the primary pathogens that cause infectious calf diarrhea, resulting in mortality and causing economic losses. Probiotics have been widely researched for their positive impact on inhibiting growth pathogenic bacteria enhancing immunity gut health as alternatives to antibiotics. This study isolated probiotic from healthy feces: Pediococcus pentosaceus SNF15 P. SNF15). In vitro assessments included character acid-producing ability, bile salt artificial gastroenteric fluid tolerance, Caco-2 adhesion, hemolysis screening, antibiotic susceptibility. Whole-genome sequencing identified immunomodulatory, antimicrobial, metabolic genes. A murine model evaluated efficacy against K99, outcomes clinical indices (fecal score, weight), histopathology (H&E), inflammatarty factor (qRT-PCR ELISA), tight junction proteins mucin (immunohistochemistry detection). Finally, 16S rRNA was performed compare composition relative abundance microbiota among different groups. demonstrated excellent performance acid production capacity, resistance, cells adhesion safety (γ-hemolysis, sensitivity) Genomic analysis revealed immune, anti-inflammatory, antagonistic pathogens, carbohydrate utilization, including secondary acid, nicotinate nicotinamide. The animal tests showed treatment protects K99 infection, evidenced by symptoms, weight loss, fecal liver atrophy, spleen enlargement occurred histological damage. Compared with CN group, supplementation strains ameliorated damage jejunum content occludin, claudin, ZO-1, MUC2 decreased levels IL-6, IL-1β, TNF-α jejunum. rDNA sequence results infection led an imbalance microbiota; proportion Firmicutes Bacteroidetes decreased, Proteobacteria increased. helps improve intestinal microbial prevents this trend. can prevent treat epithelial mucosal integrity, permeability, immune-related cytokines regulate K99-infected mice. research possesses desirable characteristics could be used a potential remit neonatal caused infection.

Language: Английский

Probiotic characteristics and whole genome sequencing of Pediococcus pentosaceus SNF15 and its protective effect on mice diarrhea induced by Escherichia coli K99 DOI Creative Commons
Yu‐Ching Su,

Mingque Feng,

Jun Tong

et al.

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: March 13, 2025

Escherichia coli ( E. col iK99) is one of the primary pathogens that cause infectious calf diarrhea, resulting in mortality and causing economic losses. Probiotics have been widely researched for their positive impact on inhibiting growth pathogenic bacteria enhancing immunity gut health as alternatives to antibiotics. This study isolated probiotic from healthy feces: Pediococcus pentosaceus SNF15 P. SNF15). In vitro assessments included character acid-producing ability, bile salt artificial gastroenteric fluid tolerance, Caco-2 adhesion, hemolysis screening, antibiotic susceptibility. Whole-genome sequencing identified immunomodulatory, antimicrobial, metabolic genes. A murine model evaluated efficacy against K99, outcomes clinical indices (fecal score, weight), histopathology (H&E), inflammatarty factor (qRT-PCR ELISA), tight junction proteins mucin (immunohistochemistry detection). Finally, 16S rRNA was performed compare composition relative abundance microbiota among different groups. demonstrated excellent performance acid production capacity, resistance, cells adhesion safety (γ-hemolysis, sensitivity) Genomic analysis revealed immune, anti-inflammatory, antagonistic pathogens, carbohydrate utilization, including secondary acid, nicotinate nicotinamide. The animal tests showed treatment protects K99 infection, evidenced by symptoms, weight loss, fecal liver atrophy, spleen enlargement occurred histological damage. Compared with CN group, supplementation strains ameliorated damage jejunum content occludin, claudin, ZO-1, MUC2 decreased levels IL-6, IL-1β, TNF-α jejunum. rDNA sequence results infection led an imbalance microbiota; proportion Firmicutes Bacteroidetes decreased, Proteobacteria increased. helps improve intestinal microbial prevents this trend. can prevent treat epithelial mucosal integrity, permeability, immune-related cytokines regulate K99-infected mice. research possesses desirable characteristics could be used a potential remit neonatal caused infection.

Language: Английский

Citations

0