Serine/threonine protein kinase mediates rifampicin resistance in Brucella melitensis through interacting with ribosomal protein RpsD and affecting antioxidant capacity DOI Creative Commons
Yaqin Yuan, Wen Ning, Junjie Chen

et al.

mSystems, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 5, 2024

ABSTRACT Brucellosis, a zoonotic disease, has re-emerged in both humans and animals, causing significant economic losses globally. Recently, an increasing number of rifampicin-resistant Brucella strains have been isolated worldwide without detectable mutations known antibiotic resistance genes. Here, this study identified the deletion serine/threonine protein kinase (STPK) gene B. melitensis as efficient trigger for rifampicin using bioinformatics predictions, transposon mutant library, mutation strains. Notably, absence STPK could increase expression ribosomal proteins genes involved sulfur metabolism reduced glutathione, decrease NADPH oxidase activity NADP + /NADPH ratio, which is associated with antioxidant capacity . Moreover, co-immunoprecipitation revealed that efficiently interact RpsD, possibly altering translation riboswitch expression. These findings demonstrate mediates by regulating to counteract reactive oxygen species induced rifampicin. Furthermore, approaches developed provide platform screening new spp., or its pathway can serve potential target drug development against spp. IMPORTANCE New via predictions whole-genome mechanisms , function interaction proteins.

Language: Английский

Brucella abortushijacks the host protein Slc2a1via the SepA effector to promote intracellular survival in macrophages DOI Creative Commons

Yuanhao Yang,

Youli Zu, Xin Wang

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 20, 2025

Abstract Brucella spp. are facultative intracellular bacteria that infect and induce brucellosis in a diverse range of mammalian hosts. The disease causes major global economic losses also is worldwide threat to public health security. Characterization bacterial host factors promote survival key for the prevention control brucellosis. In this study, we identified proteins involved Brucella abortus A19 RAW264.7 macrophage cells by liquid chromatography-mass spectrometry macrophages with or without B. infection. functions these proteins, signaling pathways which participate, domain entries enriched subcellular localization differentially-expressed were deciphered. Differential protein expression revealed Slc2a1, Glycolytic protein, was significantly upregulated infected cells. This observation confirmed qRT-PCR Western blotting studies. role Slc2a1 probed overexpressing knocking down SLC2A1 Overproduction promoted proliferation whereas knockdown inhibited bacterium. Finally, determined Secreted Effector Protein A (SepA) effector enhanced Thus, stimulates via SepA aid environment suggests may be novel antibacterial target combat

Language: Английский

Citations

0
(P)ppGpp synthetase Rel facilitates cellulose formation of biofilm by regulating glycosyltransferase in Brucella abortus DOI

Xiao Fang Liu,

Pingping Wang, Zheng Dong

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 140022 - 140022

Published: Jan. 1, 2025

Language: Английский

Citations

0
What Is the Impact of Antibiotic Resistance Determinants on the Bacterial Death Rate? DOI Creative Commons

Bruno T. S. Luz,

João S. Rebelo, Francisca Monteiro

et al.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(2), P. 201 - 201

Published: Feb. 14, 2025

Objectives: Antibiotic-resistant bacteria are widespread, with resistance arising from chromosomal mutations and genes located in the chromosome or mobile genetic elements. While determinants often reduce bacterial growth rates, their influence on death under bactericidal antibiotics remains poorly understood. When exposed to which they susceptible, typically undergo a two-phase decline: fast initial exponentially decaying phase, followed by persistent slow-decaying phase. This study examined how affect rates during both phases. Methods: We analyzed of ampicillin-exposed Escherichia coli populations strains sensitive ampicillin but resistant nalidixic acid, rifampicin, both, carrying conjugative plasmids RN3 R702. Results: Single mutants acid rifampicin decayed faster than cells early whereas double-resistant mutant exhibited prolonged survival. These contrasting impacts suggest epistatic interactions between mutations. Persistent-phase for did not differ significantly wild-type cells. In contrast, plasmid-carrying displayed distinct dynamics: R702 plasmid-bearing showed higher persistent-phase plasmid-free cells, while lower rates. Conclusions: Bactericidal may kill other more effectively Moreover, epistasis occur when different same cell, impacting potential antibiotic choice. results have significant implications optimizing eradication protocols clinical settings, as well animal health industrial food safety management.

Language: Английский

Citations

0
Serine/threonine protein kinase mediates rifampicin resistance in Brucella melitensis through interacting with ribosomal protein RpsD and affecting antioxidant capacity DOI Creative Commons
Yaqin Yuan, Wen Ning, Junjie Chen

et al.

mSystems, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 5, 2024

ABSTRACT Brucellosis, a zoonotic disease, has re-emerged in both humans and animals, causing significant economic losses globally. Recently, an increasing number of rifampicin-resistant Brucella strains have been isolated worldwide without detectable mutations known antibiotic resistance genes. Here, this study identified the deletion serine/threonine protein kinase (STPK) gene B. melitensis as efficient trigger for rifampicin using bioinformatics predictions, transposon mutant library, mutation strains. Notably, absence STPK could increase expression ribosomal proteins genes involved sulfur metabolism reduced glutathione, decrease NADPH oxidase activity NADP + /NADPH ratio, which is associated with antioxidant capacity . Moreover, co-immunoprecipitation revealed that efficiently interact RpsD, possibly altering translation riboswitch expression. These findings demonstrate mediates by regulating to counteract reactive oxygen species induced rifampicin. Furthermore, approaches developed provide platform screening new spp., or its pathway can serve potential target drug development against spp. IMPORTANCE New via predictions whole-genome mechanisms , function interaction proteins.

Language: Английский

Citations

0