Poly ADP-Ribosylation in a Plant Pathogenic Oomycete Phytophthora infestans: A Key Controller of Growth and Host Plant Colonisation

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(1), P. 29 - 29

Published: Jan. 3, 2025

ADP-ribosylation is a reversible modification of proteins and nucleic acids, which controls major cellular processes, including DNA damage repair, cell proliferation differentiation, metabolism, stress, immunity in plants animals. The involvement the life cycle Dictyostelium some filamentous fungi has also been demonstrated. However, role this process pathogenic oomycetes never addressed. Here, we show that Phytophthora infestans genome contains two PARP-like protein genes (PiPARP1 PiPARP2), provide evidence PARylation activity for one them (PiPARP2). Using dsRNA-mediated RNA silencing PiPARP2 gene chemical (pharmacological) inhibition PARP by 3-aminobenzamide (3AB) inhibitor, demonstrate critical functional mycelium growth. Virulence test on detached leaves suggests an important host plant colonisation pathogenesis. On practical level, our data suggest targeting system may constitute novel powerful approach management diseases.

Language: Английский

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The Ubiquitous Wilt-Inducing Pathogen Fusarium oxysporum—A Review of Genes Studied with Mutant Analysis

Pathogens, Journal Year: 2024, Volume and Issue: 13(10), P. 823 - 823

Published: Sept. 24, 2024

Fusarium oxysporum is one of the most economically important plant fungal pathogens, causing devastating wilt diseases on a diverse range hosts, including many key crop plants. Consequently, F. has been subject extensive research to help develop and improve protection strategies. The sequencing genome 14 years ago greatly accelerated discovery characterization genes contributing biology virulence. In this review, we summarize findings molecular mechanisms growth, reproduction, particular, focus studied through mutant analysis, covering involved in processes such as metabolism, stress tolerance, sporulation, pathogenicity, well signaling pathways that regulate them. doing so, hope present comprehensive review understanding will aid future study related species.

Language: Английский

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The secreted protein FonCHRD is essential for vegetative growth, asexual reproduction, and pathogenicity in watermelon Fusarium wilt fungus

Crop Health, Journal Year: 2024, Volume and Issue: 2(1)

Published: Oct. 25, 2024

Abstract Fungal pathogens often secrete numerous effectors to interfere with and/or suppress plant immunity promote their infection. Watermelon Fusarium wilt, caused by the soil-borne fungus oxysporum f. sp. niveum ( Fon ), is one of devastating diseases that severely affect watermelon industry. Here, we report function a candidate effector protein, FonCHRD, in . FonCHRD harbors chordin (CHRD) domain unknown and has signal peptide secretion activity. shows relatively high expression level marcoconidia inducible root tissues. Phenotypic analysis targeted deletion mutant revealed plays roles vegetative growth, asexual reproduction, conidial morphology , while it not involved spore germination as well cell wall, oxidative salt stress responses. Deletion impaired ability colonize spread within host plants, significantly reducing its virulence on watermelon. distributed across multiple compartments cells but can target apoplast space plants. inhibits INF1- Bcl2-associated X protein-triggered death defense gene transiently expressed Nicotiana benthamiana leaves. These findings suggest essential for pathogenicity modulating invasive growth spreading abilities suppressing immune

Language: Английский

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Poly(ADP-ribose) polymerase FonPARP1-catalyzed PARylation of protein disulfide isomerase FonPdi1 regulates pathogenicity of Fusarium oxysporum f. sp. niveum on watermelon

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 139046 - 139046

Published: Dec. 1, 2024

Language: Английский

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Protein PARylation: a novel regulator of fungal virulence

Trends in Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

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