Network Pharmacology and Experimental Validation Reveal Ganodermanontriol Modulates Pneumonia via TNF/NF‐κB/MAPKs Signaling Pathway DOI Creative Commons

Shizhan Deng,

Dequan Zhong,

Yonggan Dong

et al.

Food Science & Nutrition, Journal Year: 2025, Volume and Issue: 13(4)

Published: March 25, 2025

Ganoderma lucidum (Leyss. ex Fr.) Karst, commonly known as Lingzhi, has long been employed in traditional Chinese medicine for its medicinal properties, particularly alleviating respiratory issues like cough and asthma. Recognized both a therapeutic agent an edible supplement, Lingzhi is celebrated health-promoting benefits. Despite widespread use, the effectiveness of G. treating pneumonia not extensively studied, highlighting need further research. This research aimed to evaluate potential treatment uncover mechanisms behind effects, specifically examining how active constituents influence inflammatory pathways. The study utilized approaches such network pharmacology, bioinformatics, molecular docking, vivo experiments. High-performance liquid chromatography (HPLC) chromatography-mass spectrometry (LC-MS) analyses revealed eight triterpenoids lucidum, with ganodermanontriol being most prominent. Molecular docking studies anticipated interactions between these compounds target proteins, while experiments on pneumonia-induced rat models assessed efficacy ganodermanontriol. Additionally, HPLC LC-MS confirmed presence ethanol extract predominantly Network pharmacology identified key genes-including TNF, EGFR, ESR1, HIF1A, HSP90AA1, SRC-that played significant roles regulation In results demonstrated that mitigated lung tissue damage rats experimentally induced by reducing release mediators. Further mechanistic showed downregulated TNF-α inhibited NF-κB/MAPKs signaling These findings suggested holds promising anti-inflammatory targeting TNF/NF-κB/MAPKs pathway, offering novel approach.

Language: Английский

Establishment of molecular weight specific chromatogram of Ganoderma glycopeptide and its application in extract DOI

Hongjian Luo,

Shuqian Lin, Zhanxi Lin

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106191 - 106191

Published: Feb. 1, 2025

Language: Английский

Citations

0
Endophytes unleashed: natural allies for heavy metal bioremediation DOI Creative Commons
Ankur Bhardwaj

Discover Plants., Journal Year: 2025, Volume and Issue: 2(1)

Published: March 11, 2025

Language: Английский

Citations

0
Enhancement of Biomethane Yield from Spent Mushroom Substrate: Biological Pretreatment with the Chlamydospores of Trichoderma viride DOI Creative Commons

Zhu Wentao,

Xianzhi Lai, Changfa Liu

et al.

Fermentation, Journal Year: 2025, Volume and Issue: 11(3), P. 152 - 152

Published: March 18, 2025

Fungal chlamydospores are asexual spores formed by fungi under adverse conditions and could be used in biological pretreatment for biogas projects fed lignocellulosic substrates. In this study, Trichoderma viride (Tv) were as the agent to enhance methane yield of spent mushroom substrates (SMSs). Lignocellulosic composition, methanogenesis performance, anaerobic microbial communities investigated different Tv durations (0 h, 12 24 48 96 192 h). The results showed that optimal duration was cumulative reached 173.4 mL/gVS, which 16.8% higher than control. A longer h not conducive methanogenesis. Sequencing analysis directly proportional relative abundance at beginning digestion. When initial 50%, became absolute dominant fungus with an 97% fungal later stage correlation network among fungi, bacteria, archaea related 11 genera, through these taxa, affected 58% taxa whole network. Cost accounting has a net income 45.5 CNY/1000 kg SMS, is promising technology. This study provides important guidance use also promotes understanding

Language: Английский

Citations

0
Network Pharmacology and Experimental Validation Reveal Ganodermanontriol Modulates Pneumonia via TNF/NF‐κB/MAPKs Signaling Pathway DOI Creative Commons

Shizhan Deng,

Dequan Zhong,

Yonggan Dong

et al.

Food Science & Nutrition, Journal Year: 2025, Volume and Issue: 13(4)

Published: March 25, 2025

Ganoderma lucidum (Leyss. ex Fr.) Karst, commonly known as Lingzhi, has long been employed in traditional Chinese medicine for its medicinal properties, particularly alleviating respiratory issues like cough and asthma. Recognized both a therapeutic agent an edible supplement, Lingzhi is celebrated health-promoting benefits. Despite widespread use, the effectiveness of G. treating pneumonia not extensively studied, highlighting need further research. This research aimed to evaluate potential treatment uncover mechanisms behind effects, specifically examining how active constituents influence inflammatory pathways. The study utilized approaches such network pharmacology, bioinformatics, molecular docking, vivo experiments. High-performance liquid chromatography (HPLC) chromatography-mass spectrometry (LC-MS) analyses revealed eight triterpenoids lucidum, with ganodermanontriol being most prominent. Molecular docking studies anticipated interactions between these compounds target proteins, while experiments on pneumonia-induced rat models assessed efficacy ganodermanontriol. Additionally, HPLC LC-MS confirmed presence ethanol extract predominantly Network pharmacology identified key genes-including TNF, EGFR, ESR1, HIF1A, HSP90AA1, SRC-that played significant roles regulation In results demonstrated that mitigated lung tissue damage rats experimentally induced by reducing release mediators. Further mechanistic showed downregulated TNF-α inhibited NF-κB/MAPKs signaling These findings suggested holds promising anti-inflammatory targeting TNF/NF-κB/MAPKs pathway, offering novel approach.

Language: Английский

Citations

0