Noninvasive monitoring of vascular alterations in mice with acute lower limb ischemia using multimodal photoacoustic imaging DOI Creative Commons
Heng Wang, Keyi Fan,

Yijie Ning

et al.

Bioengineering & Translational Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

Abstract Acute limb ischemia (ALI), especially acute lower (ALLI), is a common clinical vascular emergency with high amputation and mortality rates. However, major challenges exist in rapidly diagnosing assessing collateral compensatory capacity, leading to appropriate treatment strategies improve preservation reduce recurrence Traditional imaging methods, such as digital subtraction angiography (DSA) ultrasound, have demands on patient kidney function operator maneuvers are unable monitor the temporal spatial variability of circulation establishment limb. In this study, we report first combined use real‐time wide‐field laser speckle (RFLSI), near‐infrared two‐zone (NIR‐II), duplex ultrasound (DUS), optical coherence tomography (OCTA) for diagnosis monitoring ALLI male C57 ICR mice. The RFLSI assesses overall perfusion, NIR‐II allows rapid circulation, DUS monitors muscle tenderness stiffness, OCTA detects microcirculation skin limb, which confirmed by histopathological testing. Overall, results study demonstrate ability accurately diagnose detect help variety acoustic devices, significant translational implications intervention decision‐making preoperatively prognostic assessment postoperatively.

Language: Английский

Noninvasive monitoring of vascular alterations in mice with acute lower limb ischemia using multimodal photoacoustic imaging DOI Creative Commons
Heng Wang, Keyi Fan,

Yijie Ning

et al.

Bioengineering & Translational Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

Abstract Acute limb ischemia (ALI), especially acute lower (ALLI), is a common clinical vascular emergency with high amputation and mortality rates. However, major challenges exist in rapidly diagnosing assessing collateral compensatory capacity, leading to appropriate treatment strategies improve preservation reduce recurrence Traditional imaging methods, such as digital subtraction angiography (DSA) ultrasound, have demands on patient kidney function operator maneuvers are unable monitor the temporal spatial variability of circulation establishment limb. In this study, we report first combined use real‐time wide‐field laser speckle (RFLSI), near‐infrared two‐zone (NIR‐II), duplex ultrasound (DUS), optical coherence tomography (OCTA) for diagnosis monitoring ALLI male C57 ICR mice. The RFLSI assesses overall perfusion, NIR‐II allows rapid circulation, DUS monitors muscle tenderness stiffness, OCTA detects microcirculation skin limb, which confirmed by histopathological testing. Overall, results study demonstrate ability accurately diagnose detect help variety acoustic devices, significant translational implications intervention decision‐making preoperatively prognostic assessment postoperatively.

Language: Английский

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