Honeybees fed D-galactose exhibit aging signs with changes in gut microbiota and metabolism

mSystems, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

ABSTRACT Honeybees ( Apis mellifera ), as social insects, exhibit complex behaviors and cognitive functions. The short lifespan stable gut microorganisms of honeybees provide certain availability a rapid high-flux animal model for aging research. This study explored the effect D-galactose, common inducer, on investigated associated effects mechanisms, with particular focus potential protective role sodium butyrate. Experimental cohorts were established follows: conventional (CV) group, D-galactose-treated (DG) butyrate-treated (SB) group. CV group was fed sucrose solution; DG D-galactose SB butyrate solution. A comprehensive assessment conducted day 15 post-treatment, including survival analysis, starvation test, motor, learning memory ability tests, malondialdehyde Smurf test. Potential mechanisms through microbiome metabolome investigated. Compared to from those in showed shortened lifespan, weaker energy storage ability, impaired learning, abilities, reduced weight, increased oxidation, disrupted barrier. These phenotypic changes microbial dysbiosis characterized by Lactobacillus enrichment diminished levels. Notably, supplementation extended honeybees’ improved their abilities damaged D-galactose. Our findings establish valuable system research highlight crucial metabolism senescence regulation. IMPORTANCE presents novel approach investigating processes establishing D-galactose-induced honeybees. demonstrate that effectively attenuates phenotypes, suggesting its therapeutic intervention age-related decline. provides unique studies highlights significant modulating progression. results contribute our understanding molecular underlying offer new insights into anti-aging strategies.

Language: Английский

Free-caged rearing modes Regulate intestinal metabolism by influencing gut microbial homeostasis

Poultry Science, Journal Year: 2024, Volume and Issue: 104(1), P. 104381 - 104381

Published: Oct. 30, 2024

Language: Английский