TRIM65 Promotes Osteogenic Differentiation by Regulating the PI3K/AKT Signaling Pathway DOI Open Access

Hong-Pu Li,

Tingting Zeng,

Zhi‐Xiang Zhou

et al.

Open Medicine Journal, Journal Year: 2024, Volume and Issue: 11(1)

Published: Sept. 11, 2024

Introduction Tripartite motif 65 (TRIM65) is a crucial regulator of cell differentiation, proliferation, migration, invasion, and carcinogenesis. However, its role in osteoporosis (OP) remains unclear. In this study, we evaluated the TRIM65 regulating osteoblast differentiation calcification. Materials Methods The during osteogenic MC3T3-E1 cells was evaluated. expression COL1A1, RUNX2, OCN examined using western blot analysis immunofluorescence staining. formation calcium nodules alizarin red Alkaline phosphatase activity ALP Results significantly elevated bone marrow mesenchymal stem cells. We demonstrated that overexpression enhanced promoted Conversely, inhibited Mechanistically, found knockdown up-regulated phosphorylated protein PI3K AKT, which contrary to results TRIM65-overexpression group. Conclusion Our research suggests an important offers therapeutic application for OP.

Language: Английский

From Genomics to Metabolomics: Molecular Insights into Osteoporosis for Enhanced Diagnostic and Therapeutic Approaches DOI Creative Commons
Qingmei Li, Jihan Wang,

Congzhe Zhao

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(10), P. 2389 - 2389

Published: Oct. 18, 2024

Osteoporosis (OP) is a prevalent skeletal disorder characterized by decreased bone mineral density (BMD) and increased fracture risk. The advancements in omics technologies—genomics, transcriptomics, proteomics, metabolomics—have provided significant insights into the molecular mechanisms driving OP. These technologies offer critical perspectives on genetic predispositions, gene expression regulation, protein signatures, metabolic alterations, enabling identification of novel biomarkers for diagnosis therapeutic targets. This review underscores potential these multi-omics approaches to bridge gap between basic research clinical applications, paving way precision medicine OP management. By integrating technologies, researchers can contribute improved diagnostics, preventative strategies, treatments patients suffering from related conditions.

Language: Английский

Citations

2
TRIM65 Promotes Osteogenic Differentiation by Regulating the PI3K/AKT Signaling Pathway DOI Open Access

Hong-Pu Li,

Tingting Zeng,

Zhi‐Xiang Zhou

et al.

Open Medicine Journal, Journal Year: 2024, Volume and Issue: 11(1)

Published: Sept. 11, 2024

Introduction Tripartite motif 65 (TRIM65) is a crucial regulator of cell differentiation, proliferation, migration, invasion, and carcinogenesis. However, its role in osteoporosis (OP) remains unclear. In this study, we evaluated the TRIM65 regulating osteoblast differentiation calcification. Materials Methods The during osteogenic MC3T3-E1 cells was evaluated. expression COL1A1, RUNX2, OCN examined using western blot analysis immunofluorescence staining. formation calcium nodules alizarin red Alkaline phosphatase activity ALP Results significantly elevated bone marrow mesenchymal stem cells. We demonstrated that overexpression enhanced promoted Conversely, inhibited Mechanistically, found knockdown up-regulated phosphorylated protein PI3K AKT, which contrary to results TRIM65-overexpression group. Conclusion Our research suggests an important offers therapeutic application for OP.

Language: Английский

Citations

0