A blueprint for contemporary studies of microbiomes

Microbiome, Journal Year: 2025, Volume and Issue: 13(1)

Published: April 8, 2025

This editorial piece co-authored by the Senior Editors at Microbiome aims to highlight current challenges in field of environmental and host-associated microbiome research. We also take opportunity clarify our expectations for articles submitted journal. At Microbiome, we are seeking studies that provide either new mechanistic insights into role microbiomes health systems or substantial conceptual technical advances. Manuscripts need meet high standards language accuracy, quality analyses, data protocol availability, including detailed reporting wet-lab silico protocols, all which can critically enhance transparency reproducibility. think such efforts essential push boundaries knowledge on a concerted, international effort.

Language: Английский

Tracing active members in microbial communities by BONCAT and click chemistry-based enrichment of newly synthesised proteins

ISME Communications, Journal Year: 2024, Volume and Issue: 4(1)

Published: Jan. 1, 2024

A comprehensive understanding of microbial community dynamics is fundamental to the advancement environmental microbiology, human health, and biotechnology. Metaproteomics, defined as analysis all proteins present within a community, provides insights into these complex systems. Microbial adaptation activity depend an important extent on newly synthesized (nP), however, distinction between nP bulk challenging. The application BONCAT with click chemistry has demonstrated efficacy in enrichment pure cultures for proteomics. However, transfer this technique communities metaproteomics proven challenging thus it not been used before. To address this, new workflow efficient specific was developed using laboratory-scale mixture labelled

Language: Английский

At what cost? The impact of bacteriophage resistance on the growth kinetics and protein synthesis of Escherichia coli

Environmental Microbiology Reports, Journal Year: 2024, Volume and Issue: 16(6)

Published: Nov. 19, 2024

Abstract Cost of bacteriophage resistance (COR) is important in explaining processes diversification and coexistence microbial communities. COR can be expressed different traits, the lack universally applicable methods to measure fitness trade‐offs makes challenging study. Due its fundamental role growth, we explored protein synthesis as a target for quantifying COR. In this study, growth kinetics three genome‐sequenced strains phage‐resistant Escherichia coli , along with phage‐susceptible wild‐type, were characterized over range glucose concentrations. Bioorthogonal non‐canonical amino acid tagging (BONCAT) was used track differences synthetic activity between wild‐type E. . Two resistant strains, levels phage susceptibility, showed mucoid phenotypes corresponding mutations genes associated Rcs phosphorelay. These isolates, however, had reduced rates potentially lower activity. Another isolate mutational profile maintained same rate increased BONCAT fluorescence, but yield lower. Together, these findings present patterns resulting from phage‐induced demonstrate potential applicability tool measuring

Language: Английский