Isolation and Characterization of Pseudomonas sp. HX1, Streptomyces luteogriseus HR40, and Streptomyces flavofungini HR77 as Promising Biocontrol Agents Against Verticillium Wilt in Hops Affected by Verticillium nonalfalfae DOI Creative Commons
Seyedehtannaz Ghoreshizadeh, Carla Calvo-Peña, Marina Ruíz-Muñoz

et al.

Horticulturae, Journal Year: 2025, Volume and Issue: 11(5), P. 459 - 459

Published: April 25, 2025

Verticillium spp. are soil-borne fungal pathogens capable of infecting over 400 plant species via their root systems, with each demonstrating a distinct host range. The primary nonalfalfae is the hop (Humulus lupulus), which becomes affected by wilt and, in severe cases, dieback. A dual screening approach was employed to isolate Streptomycetes as potential biocontrol agents from systems alongside other bacterial xylem. Three Pseudomonas strains xylem and three Streptomyces roots were selected based on vitro antifungal activity against dahliae and/or V. nonalfalfae. Their for controlling further assessed through planta assays conducted under greenhouse conditions. results demonstrated that sp. HX1, luteogriseus HR40, flavofungini HR77 significantly reduced disease severity index 32.56% compared artificially infected control plants. In contrast, commercial product Serenade® ASO, containing Bacillus amyloliquefaciens QST 713, achieved reduction 13.96%. These findings underscore promising candidates biological hops

Language: Английский

The comparative genomic analysis provides insight into the divergent inhibitory activity metabolites in pathogen-driven three Pseudomonas palleroniana strains against primary pathogens of Pseudostellaria heterophylla DOI Creative Commons
Chunfeng Huang, Xiaoai Wang,

Yanping Gao

et al.

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: April 2, 2025

Abstract Pseudostellaria heterophylla (Miq.) Pax ex et Hoffm. is a member of the Caryophyllaceae family, in which dried tuberous root well-known traditional Chinese medicine (TCM) and widespread food ingredient Asia. In recent years, large-scale cultivation P. has led to frequent infectious diseases caused by multiple pathogens. However, efficient safe approaches for preventing managing have become urgent this high-quality industrial development. Herein, culturable microbiome diseased rhizosphere soil was constructed, broad-spectrum antifungal activity Pseudomonas screened. Three palleroniana strains, B-BH16-1, B-JK4-1, HP-YBB-1B, were isolated identified with vigorous confrontation method. We employed PacBio RS II single-molecule real-time (SMRT) sequencing Illumina methods obtain genome these three isolates. Phylogenetic, synteny, ANI analysis showed that lineage between strain B-JK4-1 B-BH16-1 or HY-YBB-1B closer than HP-YBB-1B. The comparative strains HP-YBB-1B marked differences secondary metabolite biosynthesis genes among strains. Strain HP-YBB-1 produced tolaasin I/tolaasin F (23 genes), sessilin A (37 putisolvin (39 respectively. CAZyme 126, 129, 127 CAZymes genomes, auxiliary activities (AA), carbohydrate esterases (CE), glycosyl transferases (GT) categories different These results provide new insights into divergent metabolites pathogen-driven against primary pathogens .

Language: Английский

Citations

0
Isolation and Characterization of Pseudomonas sp. HX1, Streptomyces luteogriseus HR40, and Streptomyces flavofungini HR77 as Promising Biocontrol Agents Against Verticillium Wilt in Hops Affected by Verticillium nonalfalfae DOI Creative Commons
Seyedehtannaz Ghoreshizadeh, Carla Calvo-Peña, Marina Ruíz-Muñoz

et al.

Horticulturae, Journal Year: 2025, Volume and Issue: 11(5), P. 459 - 459

Published: April 25, 2025

Verticillium spp. are soil-borne fungal pathogens capable of infecting over 400 plant species via their root systems, with each demonstrating a distinct host range. The primary nonalfalfae is the hop (Humulus lupulus), which becomes affected by wilt and, in severe cases, dieback. A dual screening approach was employed to isolate Streptomycetes as potential biocontrol agents from systems alongside other bacterial xylem. Three Pseudomonas strains xylem and three Streptomyces roots were selected based on vitro antifungal activity against dahliae and/or V. nonalfalfae. Their for controlling further assessed through planta assays conducted under greenhouse conditions. results demonstrated that sp. HX1, luteogriseus HR40, flavofungini HR77 significantly reduced disease severity index 32.56% compared artificially infected control plants. In contrast, commercial product Serenade® ASO, containing Bacillus amyloliquefaciens QST 713, achieved reduction 13.96%. These findings underscore promising candidates biological hops

Language: Английский

Citations

0