Enhancing Periodontal Ligament Regeneration via PDLSC Delivery Using Electrospun PCL/Collagen/Cellulose Acetate Scaffolds and Collagen Hydrogel Incorporated with Curcumin-Loaded ZIF-8 Nanoparticles

International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 887 - 906

Published: Jan. 1, 2025

Regenerating periodontal ligament (PDL) tissue is a vital challenge in dentistry that aims to restore function and aesthetics. This study explores engineering strategy combines polycaprolactone (PCL)/collagen/cellulose acetate electrospun scaffolds with collagen hydrogels deliver curcumin-loaded ZIF-8 nanoparticles fand stem cells (PDLSCs). Scaffolds were fabricated via electrospinningand incorporated PDLSCs (CURZIF-8) developed using cross-linking. In vitro assays evaluated biocompatibility, anti-inflammatory, antioxidative properties. vivo efficacy was assessed rat PDL injury model histological ELISA analyses examining regeneration inflammatory cytokine modulation. studies demonstrated the effectively supported PDLSC viability migration. CURZIF-8 enhanced anti-inflammatory activities. showed combined scaffold-hydrogel system significantly promoted regeneration. Tissue levels of bFGF, HGF, TGF-β are crucial for repair, angiogenesis, cell proliferation evaluated. Whereas, pro-inflammatory cytokines TNF-α IL-6=were downregulated. Histological analysis confirmed formation organized structures improved bone-cementum integration arekey indicators successful The facilitates by modulating inflammation promoting pro-healing factor expression. approach shows promise advancing warrants further investigation clinical settings.

Language: Английский

---

Chronic Inflammation and Glycemic Control: Exploring the Bidirectional Link Between Periodontitis and Diabetes

Dentistry Journal, Journal Year: 2025, Volume and Issue: 13(3), P. 100 - 100

Published: Feb. 26, 2025

Periodontitis and diabetes mellitus are two highly prevalent chronic conditions that share a bidirectional relationship, significantly impacting public health. Periodontitis, gum inflammation caused by microbial dysbiosis, aggravates glycemic control in diabetics, while uncontrolled heightens periodontitis severity. These create vicious cycle, where dysbiosis mutually drive disease progression, exacerbating systemic The underlying mechanisms involve inflammation, immune dysfunction, with both diseases contributing to chain of exacerbates This relationship is significant because managing one condition can impact the other. In diabetic individuals, interventions such as periodontal therapy have shown effectiveness improving control, underscoring potential integrated strategies for these simultaneously. this review, we highlight importance deeper understanding molecular immunological interactions between essential developing therapeutic approaches, enhance quality life patient significantly.

Language: Английский

---

The Impact of Ozone on Periodontal Cell Line Viability and Function

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(2), P. 72 - 72

Published: Jan. 23, 2025

Periodontal diseases, including gingivitis and periodontitis, are chronic inflammatory conditions of the teeth’ supporting structures that can lead to progressive tissue destruction loss if left untreated. Basic treatments like scaling root planing, alone or combined with antimicrobial agents, standard care. However, increasing prevalence antibiotic resistance need for new ideas in therapy, adjunctive ozone therapy have gained attention. Ozone (O3), a triatomic oxygen molecule, is used because its strong antimicrobial, anti-inflammatory, regenerative activity and, hence, as potential tool periodontal therapy. This review use disease breaks down multifaceted mechanism which includes selective action against biofilm-associated pathogens, immunomodulatory effects on host cells, stimulation repair. O3 disrupts microbial biofilms, enhances immune cell function, promotes healing by activating Nuclear Factor Erythroid 2-Related 2 (Nrf2) Mitogen-Activated Protein Kinase (MAPK) signaling pathways regulate oxidative stress, inflammation, apoptosis. Additional findings include ability upregulate growth factors extracellular matrix proteins, significant regeneration. also discusses application lines, emphasizing impact viability, proliferation, differentiation. Advances techniques, properties O3, demonstrated clinical benefits. Challenges, standardized dosages, effective delivery systems, long-term studies, addressed ensure safe integration. dual capabilities, offers an innovative approach treatment. Future research focusing optimized protocols evidence-based guidelines essential fully realize enhancing health improving patient outcomes.

Language: Английский

---

Prevalence of red, orange, and green microbial complexes in periodontal pockets of chronic kidney disease patients on hemodialysis: a systematic review and meta-analysis

Bioscience Journal, Journal Year: 2025, Volume and Issue: 41, P. 72203 - 72203

Published: Feb. 7, 2025

To verify the prevalence of specific microorganisms red, green, and orange microbiological complexes in periodontal pockets chronic kidney disease (CKD) patients on hemodialysis. This systematic review was conducted according to PRISMA statement. The search strategy included two independent reviewers who searched PubMed, Web Science, Scopus, Cochrane, Lilacs databases August 2024, using MeSH terms keywords defined with PICOS acronym. studies were cross-sectional published from 2007-2017 English. risk bias quality evidence assessed NIH Quality Assessment Tool for Observational Cohort Cross-sectional Studies. Quantitative analysis proportion meta-analysis also performed. 4,737 initially selected, five included. study verified green complex - Aggregatibacter actinomycetemcomitans (6.69%); Prevotella intermedia (16.85%) nigrescens (37.51%); red Treponema denticola (29.11%), Porphyromonas gingivalis (49.45%), Tannerella forsythia (56.37%), most predominant microorganism. In subgingival microbiota CKD hemodialysis, showed higher rates prevalences, common pathogen.

Language: Английский

---

Analysis of oral conditions to explore subsistence strategies in the ecologically diverse environments of the Canary Islands during the Amazigh period (1st to 15th centuries CE)

Archives of Oral Biology, Journal Year: 2025, Volume and Issue: 174, P. 106236 - 106236

Published: March 12, 2025

Language: Английский

---

Gallic acid promotes M2 macrophage polarization through mitochondrial oxidative phosphorylation in periodontitis

Archives of Oral Biology, Journal Year: 2025, Volume and Issue: unknown, P. 106237 - 106237

Published: March 1, 2025

Language: Английский

---

Brazilian Organic Honeydew Reduces In Vitro and In Vivo Periodontal Disease-Related Subgingival Biofilm

Foods, Journal Year: 2025, Volume and Issue: 14(6), P. 997 - 997

Published: March 14, 2025

We investigated the antimicrobial properties and effects on bone resorption of Brazilian organic honeydew (OHD) from Bracatinga tree (Mimosa scabrella Benth.), a rare honey certified with Denomination Origin, using periodontal disease model. Antibiofilm activity was assessed subgingival biofilm adhered to Calgary device. Biofilms were treated OHD, chlorhexidine (0.12%), or vehicle twice daily for 1 min starting day 3, at concentrations 2× 10× minimum inhibitory concentration (MIC). employed ligature-induced chronic model challenged it Porphyromonas gingivalis in C57BL/6 mice. The chemical profile OHD analyzed LC-ESI-IT-MS/MS. Results evaluated by measuring loss microbial composition ligature through DNA-DNA hybridization. demonstrated significant against P. (MIC 4%, MBC 6%) reduced viability 80% vitro. In vivo, decreased populations associated disease. Chemical analysis identified seven compounds including five flavonoids two lignans. This Atlantic Forest exhibits strong potential as functional food oral health, offering promising alternative control prevention

Language: Английский

---

Efficacy of ozone therapy in dentistry with approach of healing, pain management, and therapeutic outcomes: a systematic review of clinical trials

BMC Oral Health, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 26, 2025

Ozone therapy has emerged as a promising treatment modality in dentistry due to its antimicrobial and healing properties. This systematic review aimed evaluate the recent clinical trials on ozone impact therapeutic outcomes. A comprehensive literature search was conducted across multiple databases, including Web of Science, PubMed, Scopus from January 2018 December 2024, identifying studies that investigated use dental applications. The findings demonstrated is effective improving periodontal health, soft tissue after implant surgery, reducing postoperative discomfort. combination scaling root planing with gaseous showed superior response rates. during endodontics procedures resulted reduced post-treatment pain, while ozonated materials promise management dentinal hypersensitivity. However, it not recommended restorative potential adverse effects bond strength. this supported integration into an adjunctive therapy. More research needed elucidate mechanisms, optimize application techniques, long-term outcomes for patient safety effectiveness.

Language: Английский

---

Unveiling the role of phages in shaping the periodontal microbial ecosystem

mSystems, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

ABSTRACT The oral microbiome comprises various species and plays a crucial role in maintaining the ecosystem host health. Phages are an important component of periodontal microbiome, yet our understanding phages remains limited. Here, we investigated using advanced bioinformatics tools based on genomes key periodontitis pathogens. Prophages were found to encode auxiliary genes that potentially enhance survival pathogenicity, including involved carbohydrate metabolism, antibiotic resistance, immune modulation. We observed cross-species transmission among prophages with complex network phage-bacteria interactions. Our findings suggest play shaping microbial ecosystem, influencing community dynamics progression periodontitis. IMPORTANCE In context periodontitis, ecological largely driven by interactions between bacteria their phages. While impact regulating pathogens has been increasingly recognized, modulating disease underexplored. This study reveals within contribute significantly virulence factor dissemination, metabolism. By metabolic capabilities strategies bacterial hosts, may act as critical regulators communities cavity. Understanding these prophage-mediated is essential not only for unraveling mechanisms but also developing innovative therapeutic approaches target at genetic level. These insights emphasize need more comprehensive studies risks posed pathogenicity resistance.

Language: Английский

---

Optimizing collagen-based biomaterials for periodontal regeneration: clinical opportunities and challenges

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Dec. 5, 2024

Periodontal disease (PD) is a chronic inflammatory condition that affects the teeth and their supporting tissues, ultimately culminating in tooth loss. Currently, treatment modalities, such as systemic local administration of antibiotics, serve to mitigate progression inflammation yet fall short restoring original anatomical structure physiological function periodontal tissues. Biocompatible material-based tissue engineering seems be promising therapeutic strategy for treating PD. Collagen, component extracellular matrix commonly used engineering, has been regarded biogenic material regeneration owing its high cell-activating biocompatible properties. The structural chemical similarities between collagen components oral render it candidate dental regeneration. This review explored properties current applications We also discussed recent therapies preparation techniques. scrutinizes pros cons associated with application collagen-based biomaterials PD treatment, aiming pave way future management

Language: Английский

---