Analysis of causal pathogens of mulberry bacterial blight in samples collected from eight provinces of China using culturomics and metagenomic sequencing methods

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 28, 2025

Mulberry bacterial blight (MBB) is a complex and one of the devastating diseases mulberry that causes serious reduction in yield quality mulberry. In recent years, transformation sericulture industry, production system, increasing seedling trade have resulted spread MBB to different parts China, posing major economic threat farmers industry. This study investigated occurrence eight provinces China during years 2023 2024. The disease samples were collected composition pathogenic microbiome was analyzed by combining culturomics metagenomic sequencing methods. A total 498 strains isolated identified through culturomics, then 109 suspected pathogen preliminarily screened based on data. Finally, 10 pathogens including, Pseudomonas syringae, P. fulva, fluorescens, Pantoea ananatis, Pectobacterium parvum, carotovorum, Flavobacterium fluviale, Citrobacter portucalensis, Klebsiella grimontii, Stenotrophomonas maltophilia , Koch’s postulates. Based distribution pattern changes community following infection with syringae we infer fulva are important MBB. addition, analysis meteorological data, bacteria showed adaptability environments, leading differences under climate conditions latitudes. data presented herein provides foundation for understanding occurrence, spatial mechanism its pathogens.

Language: Английский

Agricultural soil microbiomes at the climate frontier: Nutrient-mediated adaptation strategies for sustainable farming

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 295, P. 118161 - 118161

Published: April 1, 2025

The equilibrium transformation of soil microbial community dynamics and succession across various temporal spatial dimensions plays a critical role in maintaining plant adaptability. Intensive agricultural practices accelerate the communities, rendering their restoration function more vulnerable. Climate change, with its variable impacts, affects resilience communities through regulatory mediating effects. Investigating spatiotemporal context climate change offers valuable insights into developing robust resilient ecosystems. This review examines resources interactive effects on resource regulation, prediction structures allocation. Additionally, it explores mechanisms that sustain ecological systems, emphasizing application profit-averaging law.

Language: Английский