
Journal of Virological Methods, Journal Year: 2025, Volume and Issue: unknown, P. 115144 - 115144
Published: March 1, 2025
We assessed the viability of aerosolized human betacoronavirus OC43 (HCoV-OC43; ATCC VR-1558), rhinovirus-14 (RV-14; VR-284) and feline calicivirus (FCV; VR-782) as representative enveloped non-enveloped respiratory viruses mammals in indoor air under ambient conditions (relative humidity 50±10% temperature 22±2°C) using a room-sized (25 m3; 900 ft3) aerobiology chamber. All virus suspensions contained soil load to simulate presence body fluids they were separately into chamber six-jet Collison nebulizer. A muffin fan was used uniformly mix inside keep aerosols airborne. slit sampler with Petri plates containing 3% (wt./vol) gelatin collect samples. The liquefied an incubator assayed for infectious plaque-forming units (PFU). rates biological decay HCoV-OC43, RV-14 FCV 0.0052±0.00026, 0.0034±0.0027 0.0081±0.0031 (as log10 PFU/m3/min), respectively. also HEPA filter-based stand-alone purifier against experimentally device could demonstrate >3-log10 reductions three 46, 62 41minutes, Therefore, we can now investigate stability mammalian well decontamination technologies them field-relevant conditions.
Language: Английский