Mechanism of TiO2 nanotube UV-photocatalytic degradation of antibiotic resistance genes in the wastewater sludge and blocking of the transfer DOI Creative Commons
Hang Yu, Xu Zhang, Jingyi Zhao

et al.

Frontiers in Environmental Science, Journal Year: 2025, Volume and Issue: 13

Published: May 6, 2025

Antibiotic resistance genes (ARGs) in sludge propagate via horizontal gene transfer (HGT), necessitating advanced mitigation strategies. This study demonstrates TiO 2 nanotube UV photocatalysis effectively degrades ARGs (70.6–82.5% reduction) and suppresses HGT by targeting mobile genetic elements (MGEs; 93.4–97.1% removal). Hierarchical nanotubes (anatase phase) generated reactive oxygen species (ROS) inducing oxidative DNA damage cell lysis, preferentially eliminating intracellular (33.5–46.6% decline) while converting them to extracellular forms. Mobile (MGEs) ( tnpA-04 / intI1 ) were selectively fragmented ROS, outperforming HOCl-based systems. Microbial analysis revealed Proteobacteria (e.g., Kofleria as key ARG hosts, whose decline correlated with reduction p <0.05). Radiation-resistant Deinococcus dominated post-treatment communities but lacked associations, indicating non-transmissible residual risks. Spatial-specific degradation mechanisms emerged: directly chromosomal ARGs, ROS oxidized plasmid-borne MGEs, achieving dual elimination blockade. The intracellular-to-extracellular shift host-MGE decoupling confirmed transmission disruption. work establishes a paradigm for treatment, synchronizing removal environmental risk through ROS-microbe-DNA interplay.

Language: Английский

Mechanism of TiO2 nanotube UV-photocatalytic degradation of antibiotic resistance genes in the wastewater sludge and blocking of the transfer DOI Creative Commons
Hang Yu, Xu Zhang, Jingyi Zhao

et al.

Frontiers in Environmental Science, Journal Year: 2025, Volume and Issue: 13

Published: May 6, 2025

Antibiotic resistance genes (ARGs) in sludge propagate via horizontal gene transfer (HGT), necessitating advanced mitigation strategies. This study demonstrates TiO 2 nanotube UV photocatalysis effectively degrades ARGs (70.6–82.5% reduction) and suppresses HGT by targeting mobile genetic elements (MGEs; 93.4–97.1% removal). Hierarchical nanotubes (anatase phase) generated reactive oxygen species (ROS) inducing oxidative DNA damage cell lysis, preferentially eliminating intracellular (33.5–46.6% decline) while converting them to extracellular forms. Mobile (MGEs) ( tnpA-04 / intI1 ) were selectively fragmented ROS, outperforming HOCl-based systems. Microbial analysis revealed Proteobacteria (e.g., Kofleria as key ARG hosts, whose decline correlated with reduction p <0.05). Radiation-resistant Deinococcus dominated post-treatment communities but lacked associations, indicating non-transmissible residual risks. Spatial-specific degradation mechanisms emerged: directly chromosomal ARGs, ROS oxidized plasmid-borne MGEs, achieving dual elimination blockade. The intracellular-to-extracellular shift host-MGE decoupling confirmed transmission disruption. work establishes a paradigm for treatment, synchronizing removal environmental risk through ROS-microbe-DNA interplay.

Language: Английский

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