PLoS ONE,
Journal Year:
2024,
Volume and Issue:
19(7), P. e0305712 - e0305712
Published: July 19, 2024
Introduction
Circadian
rhythms
(CRs)
orchestrate
intrinsic
24-hour
oscillations
which
synchronize
an
organism’s
physiology
and
behaviour
with
respect
to
daily
cycles.
CR
disruptions
have
been
linked
Parkinson’s
Disease
(PD),
the
second
most
prevalent
neurodegenerative
disorder
globally,
are
associated
several
PD-symptoms
such
as
sleep
disturbances.
Studying
molecular
changes
of
offers
a
potential
avenue
for
unravelling
novel
insights
into
PD
progression,
symptoms,
can
be
further
used
optimization
treatment
strategies.
Yet,
comprehensive
characterization
alterations
at
expression
level
core-clock
clock-controlled
genes
in
is
still
missing.
Methods
analysis
The
proposed
study
protocol
will
characterize
profiles
circadian
obtained
from
saliva
samples
patients
controls.
For
this
purpose,
20
healthy
controls
70
recruited.
Data
clinical
assessment,
questionnaires,
actigraphy
tracking
polysomnography
collected
evaluations
repeated
follow-up
one-year
time.
We
plan
carry
out
sub-group
analyses
considering
factors
(e.g.,
biological
sex,
dosages,
or
fluctuation
symptoms),
correlate
reflected
measured
distinct
phenotypes
(diffuse
malignant
mild/motor-predominant).
Additionally,
using
NanoString
Ⓡ
multiplex
technology
on
subset
samples,
we
aim
explore
hundreds
involved
neuropathology
pathways.
Discussion
CLOCK4PD
mono-centric,
non-interventional
observational
aiming
PD.
determine
physiological
modifications
activity
patterns,
correlating
observed
changes.
Our
may
provide
valuable
intricate
interplay
between
predictor
reflecting
disease
phenotypes,
progression
outcomes.
BMC Pediatrics,
Journal Year:
2023,
Volume and Issue:
23(1)
Published: March 4, 2023
Abstract
Background
In
many
organisms,
including
humans,
the
timing
of
cellular
processes
is
regulated
by
circadian
clock.
At
molecular
level
core-clock
consists
transcriptional-translational-feedback
loops
several
genes
such
as
BMAL1
,
CLOCK
PERs
and
CRYs
generating
circa
24-h
rhythms
in
expression
about
40%
our
across
all
tissues.
Previously
these
have
been
shown
to
be
differentially
expressed
various
cancers.
Albeit
a
significant
effect
treatment
optimization
chemotherapy
paediatric
acute
lymphoblastic
leukaemia
has
previously
reported,
mechanistic
role
played
clock
remains
elusive.
Methods
To
characterize
clock,
we
will
recruit
patients
with
newly
diagnosed
collect
time
course
saliva
blood
samples,
well
single
bone
marrow
sample.
From
samples
nucleated
cells
isolated
further
undergo
separation
into
CD19
+
−
cells.
qPCR
performed
on
targeting
PER2
CRY1
.
Resulting
data
analysed
for
rhythmicity
using
RAIN
algorithm
harmonic
regression.
Discussion
best
knowledge
this
first
study
aiming
cohort
leukaemia.
future
hope
contribute
uncovering
vulnerabilities
cancers
associated
particular
adjust
accordingly,
leading
more
targeted
toxicity,
hence
decreased
systemic
toxicities.
Archives of Toxicology,
Journal Year:
2024,
Volume and Issue:
98(5), P. 1485 - 1498
Published: March 14, 2024
Accumulating
evidence
indicates
that
chronic
circadian
rhythm
disruption
is
associated
with
the
development
of
neurodegenerative
diseases
induced
by
exposure
to
neurotoxic
chemicals.
Herein,
we
examined
relationship
between
cellular
and
cytotoxicity
in
neural
cells.
Moreover,
evaluated
potential
application
an
vitro
assay
determining
as
a
sensitive
early
marker
neurotoxicant-induced
adverse
effects.
To
explore
these
objectives,
established
using
human
glioblastoma
(U87
MG)
cells
stably
transfected
reporter
vector
(PER2-dLuc)
determined
lowest-observed-adverse-effect
levels
(LOAELs)
several
common
neurotoxicants.
Additionally,
LOAEL
each
compound
on
multiple
endpoints
(nuclear
size
[NC],
mitochondrial
membrane
[MMP],
calcium
ions,
or
lipid
peroxidation)
multiparametric
high-content
screening
(HCS)
U87
MG
treated
same
neurotoxicants
for
24
72
h.
Based
our
findings,
most
chemicals
was
slightly
higher
than
indicators
detected
HCS,
MMP
first
h
closest
disruption.
Dietary
antioxidants
(methylselenocysteine
N-acetyl-l-cysteine)
prevented
restored
Our
results
suggest
occurs
much
cytotoxic
events
during
disease
development.
warrants
further
evaluation
tool
PLoS ONE,
Journal Year:
2024,
Volume and Issue:
19(7), P. e0305712 - e0305712
Published: July 19, 2024
Introduction
Circadian
rhythms
(CRs)
orchestrate
intrinsic
24-hour
oscillations
which
synchronize
an
organism’s
physiology
and
behaviour
with
respect
to
daily
cycles.
CR
disruptions
have
been
linked
Parkinson’s
Disease
(PD),
the
second
most
prevalent
neurodegenerative
disorder
globally,
are
associated
several
PD-symptoms
such
as
sleep
disturbances.
Studying
molecular
changes
of
offers
a
potential
avenue
for
unravelling
novel
insights
into
PD
progression,
symptoms,
can
be
further
used
optimization
treatment
strategies.
Yet,
comprehensive
characterization
alterations
at
expression
level
core-clock
clock-controlled
genes
in
is
still
missing.
Methods
analysis
The
proposed
study
protocol
will
characterize
profiles
circadian
obtained
from
saliva
samples
patients
controls.
For
this
purpose,
20
healthy
controls
70
recruited.
Data
clinical
assessment,
questionnaires,
actigraphy
tracking
polysomnography
collected
evaluations
repeated
follow-up
one-year
time.
We
plan
carry
out
sub-group
analyses
considering
factors
(e.g.,
biological
sex,
dosages,
or
fluctuation
symptoms),
correlate
reflected
measured
distinct
phenotypes
(diffuse
malignant
mild/motor-predominant).
Additionally,
using
NanoString
Ⓡ
multiplex
technology
on
subset
samples,
we
aim
explore
hundreds
involved
neuropathology
pathways.
Discussion
CLOCK4PD
mono-centric,
non-interventional
observational
aiming
PD.
determine
physiological
modifications
activity
patterns,
correlating
observed
changes.
Our
may
provide
valuable
intricate
interplay
between
predictor
reflecting
disease
phenotypes,
progression
outcomes.
