Exploring the Wound Healing Potential of Hispidin DOI Open Access
Yi-Shan Liu,

Mei-Chou Lai,

Tang-Yao Hong

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(18), P. 3161 - 3161

Published: Sept. 19, 2024

Background: Hispidin, a polyphenol component mainly derived from the medicinal mushroom species Phellinus and Inonotus, shows promise for biomedical applications, yet its potential in wound healing remains largely unexplored. This research investigates effects of hispidin through vitro vivo experiments, while also evaluating antimicrobial properties safety profile. Methods: In scratch assays were conducted to evaluate impact on migration NIH-3T3 cells. The was assessed rats using excision wounds, dead space linear incisions, treated with various topical ointments including simple ointment, 2.5% (w/w) 5% 0.2% nitrofurazone administered at 0.2 g daily 14 days. Results: Hispidin demonstrated particularly effective against Staphylococcus epidermidis. enhanced cell viability, promoted closure assays, correlating increased levels FGF21, TGF-β1, EGF, VEGF. models, ointment improved contraction, epithelialization, tissue regeneration, fibroblast activity, angiogenesis. granulation reduced pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) lipid peroxidation, increasing anti-inflammatory (IL-10) antioxidant activities (SOD, GPx, CAT), along connective markers like hydroxyproline, hexosamine, hexuronic acid. breaking strength incision models. Acute dermal toxicity studies indicated no adverse 2000 mg/kg. Conclusions: These findings highlight hispidin’s care, demonstrating antimicrobial, regenerative, properties.

Language: Английский

Gelatin Nanoemulsion-Based Co-Delivery of Terbinafine and Essential Oils for Treatment of Candida albicans Biofilms DOI Creative Commons
Muhammad Aamir Hassan, Sàdaf Noor, Jungmi Park

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(1), P. 127 - 127

Published: Jan. 9, 2025

Fungal infections represent a significant global health challenge. Candida albicans is particularly widespread pathogen, with both molecular and biofilm-based mechanisms making it resistant to or tolerant of available antifungal drugs. This study reports combination therapy, active against C. albicans, utilizing terbinafine essential oils incorporated into gelatin-based nanoemulsion system (T-GNE). Eugenol methyl eugenol/terbinafine T-GNEs had an additive efficacy, while carvacrol (CT-GNE) worked synergistically terbinafine, providing effective treatment minimal mammalian cell toxicity. Confocal microscopy demonstrated that CT-GNE penetrated the dense biofilm disrupted fungal membrane. Overall, in GNE provided promising for biofilms.

Language: Английский

Citations

0
Development of a Tri-Species Wound Model for Studying Fungal-Bacterial Interactions and Antimicrobial Therapies DOI Creative Commons

S.A. Sørensen,

Lasse Kvich, Yijuan Xu

et al.

Biofilm, Journal Year: 2025, Volume and Issue: 9, P. 100256 - 100256

Published: Jan. 21, 2025

Language: Английский

Citations

0
Biosensors integrated within wearable devices for monitoring chronic wound status DOI Creative Commons
Sabine Szunerits, Rabah Boukherroub, Christoph Kleber

et al.

APL Bioengineering, Journal Year: 2025, Volume and Issue: 9(1)

Published: Feb. 4, 2025

Slowly healing wounds significantly affect the life quality of patients in different ways, due to constant pain, unpleasant odor, reduced mobility up social isolation, and personal frustration. While remote wound management has become more widely accepted since COVID-19 pandemic, delayed treatment remains frequent results several related complications. As inappropriate notably diabetic foot ulcers is linked a high risk amputation, effective patient-centered manner important be implemented. The integration diagnostic devices into bandages under way, owing advancements materials science nanofabrication strategies as well innovation communication technologies together with machine learning data-driven assessment tools. Leveraging advanced analytical approaches around local pH, temperature, pressure, biomarker sensing expected facilitate adequate treatment. state-of-the-art time-resolved monitoring status by quantifying key physiological parameters biomarkers' concentration presented herewith. A special focus will given smart on-demand delivery capabilities for improved management.

Language: Английский

Citations

0
Pressure injuries and biofilms: Microbiome, model systems and therapies DOI Creative Commons

Fahad Kabir,

Deborah Bow Yue Yung, Waleska Stephanie da Cruz Nizer

et al.

Wound Repair and Regeneration, Journal Year: 2025, Volume and Issue: 33(1)

Published: Jan. 1, 2025

Abstract Chronic wounds have emerged as significant clinical problems owing to their increasing incidence and greater recognition of associated morbidity socio‐economic burden. They are defined that do not progress normally through the stages healing in a timely and/or orderly manner. Pressure injuries, particular, represent serious problem for patients who elderly or limited mobility, such wheelchair users those spend most day bed. These injuries often result from prolonged pressure exerted on skin over bone. Treatment is complex costly. Emerging evidence suggests injury microbiome plays vital role chronic wound formation delaying healing. Additionally, antibiotics fail due resistant biofilms emergence antimicrobial‐resistant bacteria. In this review, we will summarise current knowledge on: (a) microbiomes injuries; (b) vitro vivo model systems study (c) therapies novel treatment approaches. Understanding interactions between microbes host immune system provide valuable insights improve patient outcomes.

Language: Английский

Citations

0
Mixture Containing 5% Polysaccharide Extract of Cerioporus squamosus (Huds.) Quélet, 5% Dexpanthenol, and 0.2% Hyaluronic Acid Shows In Vitro and In Vivo Wound Healing Properties DOI Creative Commons
Jovana Petrović, Tamara Carević, Jasmina Glamočlija

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(3), P. 416 - 416

Published: March 15, 2025

Background: This study explores wound healing and the antimicrobial potential of a natural formulation containing polysaccharide extract from Cerioporus squamosus, hyaluronic acid, dexpanthenol. Methods: Wound effects were assessed using HaCaT keratinocytes, while activity was evaluated against human skin pathogens microdilution assay. In vitro cytotoxicity tests ensured safety, whereas in vivo further investigated an animal model. Gene expression analysis performed to assess molecular mechanisms involved. Results: The unique glucan composition C. squamosus (15.38% α-glucans 7.91% β-glucans) deviated typical mushroom profiles, warranting exploration its bioactivity. polysaccharides promoted 25.35% closure after 24 hours, three-component achieved 35.81% closure. Antibacterial showed minimum inhibitory concentration (MIC) 0.44–1.75 mg/mL bactericidal (MBCs) 0.88–3.50 mg/mL, antifungal ranged 0.22 0.44 (MICs) 0.88 (minimum fungicidal concentration—MFC). data that 60% treated wounds fully closed by day 11, despite no statistically significant difference control. However, gene highlighted VEGF collagen upregulation, indicating enhancement on level. Conclusions: novel demonstrated consistent properties, supporting as safe effective treatment for chronic acute wounds.

Language: Английский

Citations

0
Sub‐Micrometer Polycaprolactone and Polyethylene Glycol‐Based Fiber Mats With Iodine and Its Potential for Wound Healing DOI Creative Commons
Agnes Chacor de Figueiredo, Bruna Nunes Teixeira, Javier Mauricio Anaya‐Mancipe

et al.

Journal of Biomedical Materials Research Part A, Journal Year: 2025, Volume and Issue: 113(4)

Published: April 1, 2025

ABSTRACT This study introduces an innovative approach to developing advanced antimicrobial wound dressings by engineering fiber mats of polycaprolactone (PCL) and polycaprolactone/polyethylene glycol (PCL/PEG) loaded with iodine using solution blow spinning (SBS). The exhibited a unique morphology, combining fibers beads, average diameters 570 nm for 12% (w/v) PCL 470 1% PEG in PCL, bead 11.34 μm 10.43 μm, respectively. incorporation rendered the hydrophilic significantly enhanced their swelling capacity, which is essential exudate management. Remarkably, was incorporated at concentration specifically optimized achieve its minimum inhibitory (MIC) against range microorganisms while preserving structural integrity release profile mats. While facilitated higher initial rate, equilibrium achieved after 5 days. UV–Vis spectroscopy unveiled formation transient complexes both PEG, underscoring functional synergy. Biological assays revealed that PCL/PEG 4.26 mg/mL (PCL/PEG 3MIC) potent efficacy gram‐positive gram‐negative bacteria, as well fungi, alongside significant vitro healing potential. These findings underscore promise iodine‐loaded platform next‐generation care, effective infection control capabilities.

Language: Английский

Citations

0
Formulation and characterization of polyvinyl alcohol/chitosan composite nanofiber co-loaded with silver nanoparticle & luliconazole encapsulated poly lactic-co-glycolic acid nanoparticle for treatment of diabetic foot ulcer DOI

Manjit Manjit,

Manish Kumar, Abhishek Jha

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 258, P. 128978 - 128978

Published: Dec. 24, 2023

Language: Английский

Citations

10
Studies on bioactivities of Manuka and regional varieties of honey for their potential use as natural antibiotic agents for infection control related to wound healing and in pharmaceutical formulations DOI Creative Commons
Divakar Dahiya,

Caoimhin Mackin,

Poonam Singh Nee Nigam

et al.

AIMS Microbiology, Journal Year: 2024, Volume and Issue: 10(2), P. 288 - 310

Published: Jan. 1, 2024

Presently, most of the reported infections are a bacterial origin; however, this leads to limit within literature and research around caused by fungal pathogens, which now developing resistance antibiotic medicines. Of natural antimicrobial agents, honey has been observed with demonstrable highly exploitable infection control related wound healing properties; therefore, it incorporated into many standard pharmaceutical formulations. Generally, these products utilize pure sample as bioactive ingredient in product purposely designed for convenience application. This article aims review information available from published reports on various bioactivities variety medical-grade products, including manuka other conventional non-manuka types sourced different floral geographical regions. Additionally, highlights activities tested against pathogenic strains bacteria, yeast fungi, their applications formulation healthcare products.

Language: Английский

Citations

2
Approach to the Atypical Wound DOI

Sarah Becker,

Shannon Kody, Nicole Fett

et al.

American Journal of Clinical Dermatology, Journal Year: 2024, Volume and Issue: 25(4), P. 559 - 584

Published: May 14, 2024

Language: Английский

Citations

2
Fungal Enzyme‐Responsive Hydrogel Drug Delivery Platform for Triggered Antifungal Release DOI

Noel Vera‐González,

Carly Deusenbery,

Veronica LaMastro

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(30)

Published: Aug. 29, 2024

Abstract Fungal infections can lead to debilitating consequences if they are not treated effectively. Antifungal drugs used treat these be toxic and overuse contributes growing antifungal resistance. Candida spp., particularly C. albicans , implicated in a majority of infections. Virulent produce secreted aspartic proteases (Saps) that aid pathogen tissue invasion proliferation at an infected site. Here, fungi‐responsive hydrogels developed degrade the presence Saps provide triggered release encapsulated liposomal antifungals. The hydrogel backbone incorporates Sap‐cleavable peptide sequence enabling Sap‐responsive degradation. Hydrogels found effectively extracted from . Encapsulated antifungals show similar kinetics as degradation products Saps, supporting degradation‐dependent mechanism. liposome‐loaded responsive exhibit successful eradication cultures remain stable sterile murine wound fluid. Finally, no significant cytotoxicity is observed for fibroblast cells red blood exposed products. These have potential local, on‐demand delivery drugs, effective treatment fungal while helping limit unnecessary exposure therapeutics.

Language: Английский

Citations

2