Honeybees fed D-galactose exhibit aging signs with changes in gut microbiota and metabolism

mSystems, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

ABSTRACT Honeybees ( Apis mellifera ), as social insects, exhibit complex behaviors and cognitive functions. The short lifespan stable gut microorganisms of honeybees provide certain availability a rapid high-flux animal model for aging research. This study explored the effect D-galactose, common inducer, on investigated associated effects mechanisms, with particular focus potential protective role sodium butyrate. Experimental cohorts were established follows: conventional (CV) group, D-galactose-treated (DG) butyrate-treated (SB) group. CV group was fed sucrose solution; DG D-galactose SB butyrate solution. A comprehensive assessment conducted day 15 post-treatment, including survival analysis, starvation test, motor, learning memory ability tests, malondialdehyde Smurf test. Potential mechanisms through microbiome metabolome investigated. Compared to from those in showed shortened lifespan, weaker energy storage ability, impaired learning, abilities, reduced weight, increased oxidation, disrupted barrier. These phenotypic changes microbial dysbiosis characterized by Lactobacillus enrichment diminished levels. Notably, supplementation extended honeybees’ improved their abilities damaged D-galactose. Our findings establish valuable system research highlight crucial metabolism senescence regulation. IMPORTANCE presents novel approach investigating processes establishing D-galactose-induced honeybees. demonstrate that effectively attenuates phenotypes, suggesting its therapeutic intervention age-related decline. provides unique studies highlights significant modulating progression. results contribute our understanding molecular underlying offer new insights into anti-aging strategies.

Language: Английский

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Sodium butyrate activates the K_ATP channels to regulate the mechanism of Parkinson's disease microglia model inflammation

Immunity Inflammation and Disease, Journal Year: 2024, Volume and Issue: 12(3)

Published: March 1, 2024

Parkinson's disease (PD) is a common neurodegenerative disorder. Microglia-mediated neuroinflammation has emerged as an involving mechanism at the initiation and development of PD. Activation adenosine triphosphate (ATP)-sensitive potassium (K

Language: Английский

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Implications of Butyrate Signaling Pathways on the Motor Symptomatology of Parkinson’s Disease and Neuroprotective Effects—Therapeutic Approaches: A Systematic Review

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8998 - 8998

Published: Aug. 19, 2024

Parkinson's Disease (PD) is a prevalent neurodegenerative disorder characterized by motor and non-motor symptoms. Emerging evidence suggests that gut microbiota alterations, specifically involving short-chain fatty acids (SCFAs) like butyrate, may influence PD pathogenesis symptomatology. This Systematic Review aims to synthesize current research on the role of butyrate in modulating symptoms its neuroprotective effects PD, providing insights into potential therapeutic approaches. A systematic literature search was conducted April 2024 across databases, including ScienceDirect, Scopus, Wiley, Web Science, for studies published between 2000 2024. Keywords used were "neuroprotective AND (Parkinson disease OR symptoms)". Four authors independently screened titles, abstracts, full texts, applying inclusion criteria focused investigating regulation total 1377 articles identified, with 40 selected full-text review 14 meeting criteria. Data extraction performed study population, models, methodology, intervention details, outcomes. Quality assessment using SYRCLE RoB tool highlighted variability quality, some biases noted allocation concealment blinding. Findings indicate has significant impact improving offers benefits models. The modulation enhance levels presents promising strategy symptom management.

Language: Английский

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α-Synuclein expression in response to bacterial ligands and metabolites in gut enteroendocrine cells: an in vitro proof of concept study

Brain Communications, Journal Year: 2023, Volume and Issue: 5(6)

Published: Jan. 1, 2023

Caudo-rostral migration of pathological forms α-synuclein from the gut to brain is proposed as an early feature in Parkinson's disease pathogenesis, but underlying mechanisms remain unknown. Intestinal epithelial enteroendocrine cells sense and respond numerous luminal signals, including bacterial factors, transmit this information via enteric nervous system vagus nerve. There evidence that bacteria composition their metabolites change patients, these alterations can trigger pathology animal models disorder. Here, we investigated effect toll-like receptor free fatty acid agonists on intracellular level its release using mouse secretin tumour cell line 1 cells. Secretin were treated for 24 or 48 h with (toll-like 4 selective lipopolysaccharide; 2 Pam3CysSerLys4) 2/3 butyrate, propionate acetate. The antagonists agonists' effects after hours was also investigated. protein measured lysates culture media by western blot enzyme-linked immunosorbent assay. necrosis factor messenger RNA quantitative reverse transcription real-time polymerase chain reaction. Stimulation significantly increased amount into medium. Both reduced each receptor. Toll-like transcription, effectively inhibited corresponding antagonists. Elevated increases likelihood aggregation conversion toxic forms. Factors derived induce accumulation provide support a mechanism which exposure specific factors found dysbiosis might facilitate gut.

Language: Английский

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New insights into sodium phenylbutyrate as a pharmacotherapeutic option for neurological disorders

Synapse, Journal Year: 2024, Volume and Issue: 78(4)

Published: May 31, 2024

Abstract Neurological disorders (NDs) are diseases of the central and peripheral nervous systems that affect more than one billion people worldwide. The risk developing an ND increases with age due to vulnerability different organs genetic, environmental, social changes consequently cause motor cognitive deficits disable person from their daily activities individual productivity. Intrinsic factors (genetic factors, age, gender) extrinsic (addictions, infections, or lifestyle) favor persistence systemic inflammatory processes contribute evolution NDs. Neuroinflammation is recognized as a common etiopathogenic factor ND. study new pharmacological options for treatment should focus on improving characteristic symptoms attacking specific molecular targets allow delay damage such neuroinflammation, oxidative stress, cellular metabolic dysfunction, deregulation transcriptional processes. In this review, we describe possible role sodium phenylbutyrate (NaPB) in pathogenesis Alzheimer's disease, hepatic encephalopathy, aging, Parkinson's Huntington's amyotrophic lateral sclerosis; addition, mechanism action NaPB its beneficial effects have been shown various vivo vitro studies any

Language: Английский

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Food-breastmilk combinations alter the colonic microbiome of weaning infants: an in silico study

mSystems, Journal Year: 2024, Volume and Issue: 9(9)

Published: Aug. 27, 2024

The introduction of solid foods to infants, also known as weaning, is a critical point for the development complex microbial community inhabiting human colon, impacting host physiology in infancy and later life. This research investigated

Language: Английский

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Buty and the beast: the complex role of butyrate in Parkinson’s disease

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: April 17, 2024

Parkinson's disease (PD) is a complex neurodegenerative which often associated with gastrointestinal (GI) dysfunction. The GI tract home to wide range of microorganisms, among bacteria, that can influence the host through various mechanisms. Products produced by these bacteria act in gut but also exert effects brain via what now well established be microbiota-gut-brain axis. In those PD gut-bacteria composition found different non-PD individuals. addition compositional changes, metabolic activity gut-microbiota changed PD. Specifically, it reported key producers short chain fatty acids (SCFAs) as concentration SCFAs themselves are altered stool and blood These SCFAs, butyrate, essential nutrients for major energy source epithelial cells tract. Additionally, butyrate plays role regulating responses particularly relation inflammation. Studies have demonstrated reduction levels critical onset progression Furthermore, has been shown restoring methods such probiotics, prebiotics, sodium supplementation, fecal transplantation beneficial effect on both motor non-motor outcomes disease. This review presents an overview evidence corresponding metabolite production PD, particular focus SCFA butyrate. presenting current studies regarding clinical preclinical reports, possibility target using microbiome based approaches therapeutic context discussed.

Language: Английский

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Dietary Flaxseed and Flaxseed Oil Differentially Modulate Aspects of the Microbiota Gut–Brain Axis Following an Acute Lipopolysaccharide Challenge in Male C57Bl/6 Mice

Nutrients, Journal Year: 2023, Volume and Issue: 15(16), P. 3542 - 3542

Published: Aug. 11, 2023

The microbiota gut-brain axis (mGBA) is an important contributor to mental health and neurological mood disorders. Lipopolysaccharides (LPS) are endotoxins that components of Gram-negative bacteria cell walls have been widely shown induce both systemic neuro-inflammation. Flaxseed (Linum usitatissimum) oilseed rich in fibre, n3-poly-unsaturated fatty acid (alpha-linolenic (ALA)), lignan, secoisolariciresinol diglucoside, which all can beneficial effects across varying aspects the mGBA. objective this study was determine potential for dietary supplementation with flaxseed or oil attenuate LPS-induced inflammation through modulation In study, 72 5-week-old male C57Bl/6 mice were fed one three isocaloric diets 3 weeks: (1) AIN-93G basal diet (BD), (2) BD + 10% (FS), (3) 4% FS (FO). Mice then injected LPS (1 mg/kg i.p) saline (n = 12/group) samples collected 24 h post-injection. Dietary FS, but not FO, partially attenuated (serum TNF-α IL-10) neuro-inflammation (hippocampal and/or medial prefrontal cortex IL-10, TNF-α, IL-1β mRNA expression), had no effect on sickness nest-building behaviours. FS-fed enhanced fecal microbial diversity increased relative abundance groups (i.e., Lachnospiraceae, Bifidobacterium, Coriobacteriaceae), reduced Akkermansia muciniphila, production short-chain acids (SCFAs), may play a role its anti-inflammatory response. Overall, highlights inflammation, part intestinal microbiota, be solely driven by ALA-rich component.

Language: Английский

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Sodium Butyrate Ameliorates Postoperative Delirium by Regulating Gut Microbiota Dysbiosis to Inhibit Astrocyte Activation in Aged Mice

Neurochemical Research, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 28, 2024

Language: Английский

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Gut Microbiota Defined Epigenomes of Alzheimer’s and Parkinson’s Diseases Reveal Novel Targets for Therapy

Epigenomics, Journal Year: 2023, Volume and Issue: 16(1), P. 57 - 77

Published: Dec. 13, 2023

The origins of Alzheimer's disease (AD) and Parkinson's (PD) involve genetic mutations, epigenetic changes, neurotoxin exposure gut microbiota dysregulation. microbiota's dynamic composition its metabolites influence intestinal blood-brain barrier integrity, contributing to AD PD development. This review explores protein misfolding, aggregation links in pathogenesis. It also highlights the role a leaky microbiota-gut-brain axis promoting these diseases through inflammation-induced alterations. In addition, we investigate potential diet, probiotics transplantation for preventing treating via modifications, along with discussion related current challenges future considerations. These approaches offer promise translating research findings into practical clinical applications.

Language: Английский

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