Antibiotics-induced dysbiosis impacts dendritic morphology of adult mouse cortical interneurons

Frontiers in Neuroanatomy, Journal Year: 2025, Volume and Issue: 19

Published: March 7, 2025

Introduction A growing body of evidence suggests that the gut microbiome may contribute to changes in brain morphology. The microbiota-gut-brain axis (MGBA) has been shown influence neurogenesis, axon myelination, and synapse structure. However, it remains unclear whether MGBA can morphology density inhibitory GABAergic interneurons. aim this study was determine antibiotic-induced dysbiosis (AID) is associated with alterations dendritic interneurons medial entorhinal cortex (mEC), somatosensory (SSC), motor (MC), hippocampus (Hp). Methods cohort six-month-old GAD-67-EGFP transgenic mice treated an antibiotic cocktail for two weeks, resulting as validated by collecting stool samples at baseline after treatment, then using next-generation sequencing 16S ribosomal RNA. Results results demonstrate proposed model effectively exhibited defining features dysbiosis, including a significant reduction diversity, expansion pathobionts, loss beneficial microbes. AID group showed different areas. mean length segments SSC Hp were found be significantly decreased, while no such decrease observed mEC or MC. Furthermore, decreased mEC, Hp, areas, change MC area. Discussion interneuron dysfunction plays role pathogenesis neurological disease. findings suggest potentially influences interneurons, which development disorders.

Language: Английский

Gastrointestinal (GI)-Lung-Brain Axis

Seminars in Fetal and Neonatal Medicine, Journal Year: 2025, Volume and Issue: unknown, P. 101639 - 101639

Published: April 1, 2025

Language: Английский