Dendritic Morphology of Developing Hippocampal Neurons in <i>Cyp11a1</i> Null Mice DOI Creative Commons

Hao-Hua Jiang,

Tzu-Hsuan Wu,

Li‐Jen Lee

et al.

Developmental Neuroscience, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 15

Published: July 17, 2024

Neurosteroids have a variety of neurological functions, such as neurite growth, neuroprotection, myelination, and neurogenesis. P450scc, encoded by CYP11A1 gene, is the cholesterol side chain cleavage enzyme that catalyzes first rate-limiting step in steroidogenesis. In this study, we examine dendritic morphology developing hippocampal neurons Cyp11a1 null mice at P15, critical period for synapse formation maturation.

Language: Английский

Loss of Sigma-2 Receptor/TMEM97 Is Associated with Neuropathic Injury-Induced Depression-Like Behaviors in Female Mice DOI Creative Commons
Veronica M. Hong,

Avaneesh Rade,

Shen M. Yan

et al.

eNeuro, Journal Year: 2024, Volume and Issue: 11(7), P. ENEURO.0488 - 23.2024

Published: June 12, 2024

Previous studies have shown that ligands bind to sigma-2 receptor/TMEM97 (s 2 R/TMEM97), a transmembrane protein, anxiolytic/antidepressant-like properties and relieve neuropathic pain-like effects in rodents. Despite medical interest s R/TMEM97, little affective pain behavioral characterization has been done using transgenic mice, which limits the development of R/TMEM97 as viable therapeutic target. Using wild-type (WT) global Tmem97 knock-out (KO) we sought identify contribution modulating behaviors battery assays, including open field, light/dark preference, elevated plus maze, forced swim test, tail suspension mechanical sensitivity tests. Our results demonstrate female KO mice show less anxiety-like depressive-like preference tests but not an at baseline. We next performed spared nerve injury WT assess role pain-induced anxiety depression. developed prolonged phenotype when tested 10 weeks after females. plays naive animals with significant change presence mice. Overall, these data could be target alleviate comorbidities disorders.

Language: Английский

Citations

2
Understanding the Antidepressant Mechanisms of Acupuncture: Targeting Hippocampal Neuroinflammation, Oxidative Stress, Neuroplasticity, and Apoptosis in CUMS Rats DOI Creative Commons
Jianguo Li, Xinhong Wu,

Simin Yan

et al.

Molecular Neurobiology, Journal Year: 2024, Volume and Issue: 62(4), P. 4221 - 4236

Published: Oct. 18, 2024

Depression is recognized globally as one of the most intractable diseases, and its complexity diversity make treatment extremely challenging. Acupuncture has demonstrated beneficial effects in various psychiatric disorders. However, underlying mechanisms acupuncture's antidepressant action, particularly depression, remain elusive. Therefore, this study aimed to investigate acupuncture on chronic unpredictability stress (CUMS)-induced depressive symptoms rats further elucidate molecular mechanisms. All were exposed CUMS two stressors every day for 28 days, except control group. One hour before CUMS, given a with acupuncture, electroacupuncture, sham-acupuncture, or fluoxetine (2.1 mg/kg). Behavioral tests biological detection methods conducted sequence evaluate depression-like phenotype rats. The findings demonstrate that therapy effectively ameliorated behavior induced by Additionally, exerted restorative effect alterations levels malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), brain-derived neurotrophic factor (BDNF), cyclic AMP response element-binding protein (CREB), postsynaptic density95 (PSD95), gamma-aminobutyric acid (GABA), acetylcholine (ACh). our indicate also modulates ERK Caspase-3 apoptotic pathways hippocampus This suggests may play potential preventive role regulating hippocampal neuroinflammatory response, oxidative stress, processes, enhancing synaptic plasticity.

Language: Английский

Citations

2
Astrocytes in the rostral ventromedial medulla mediate the analgesic effect of electroacupuncture in a rodent model of chemotherapy-induced peripheral neuropathic pain DOI
Xuejiao Chen, Wen‐Li Mi,

Tianchi Gao

et al.

Pain, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 15, 2024

Abstract Chemotherapy-induced peripheral neuropathic pain aggravates cancer survivors' life burden. Electroacupuncture (EA) has exhibited promising analgesic effects on in previous studies. We investigated whether EA was effective a paclitaxel-induced mouse model. further explored the functional role of astrocytes rostral ventromedial medulla (RVM), well-established modulation center, process as well effect EA. found that paclitaxel induced mechanical allodynia, astrocytic calcium signaling, and neuronal activation RVM spinal cord, which could be suppressed by treatment. effectively alleviated attenuated chemogenetic RVM. In addition, inhibiting activity using either IP 3 R2 knockout (IP KO) mice or microinjection AAV-mediated hPMCA2 w/b into to reduce non–IP R2-dependent Ca 2+ signaling an pain, mimicked effect. The current study revealed pivotal mediating chemotherapy-induced pain.

Language: Английский

Citations

2
Dendritic Morphology of Developing Hippocampal Neurons in <i>Cyp11a1</i> Null Mice DOI Creative Commons

Hao-Hua Jiang,

Tzu-Hsuan Wu,

Li‐Jen Lee

et al.

Developmental Neuroscience, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 15

Published: July 17, 2024

Neurosteroids have a variety of neurological functions, such as neurite growth, neuroprotection, myelination, and neurogenesis. P450scc, encoded by CYP11A1 gene, is the cholesterol side chain cleavage enzyme that catalyzes first rate-limiting step in steroidogenesis. In this study, we examine dendritic morphology developing hippocampal neurons Cyp11a1 null mice at P15, critical period for synapse formation maturation.

Language: Английский

Citations

0