Nrf2 as a regulator of mitochondrial function: Energy metabolism and beyond

Free Radical Biology and Medicine, Journal Year: 2022, Volume and Issue: 189, P. 136 - 153

Published: July 31, 2022

Language: Английский

---

The Guide to Dendritic Spikes of the Mammalian Cortex In Vitro and In Vivo

Neuroscience, Journal Year: 2022, Volume and Issue: 489, P. 15 - 33

Published: Feb. 17, 2022

Half a century since their discovery by Llinás and colleagues, dendritic spikes have been observed in various neurons different brain regions, from the neocortex cerebellum to basal ganglia. Dendrites exhibit terrifically diverse but stereotypical repertoire of spikes, sometimes specific subregions dendrite. Despite prevalence, we only glimpse into role behaving animal. This article aims survey full range found excitatory inhibitory neurons, compare themin vivoversusin vitro, discuss new studies describing human cortex. We focus on neocortical hippocampal present roadmap identify understand broader single-cell computation.

Language: Английский

---

Bisphenol A (BPA) and neurological disorders: An overview

The International Journal of Biochemistry & Cell Biology, Journal Year: 2024, Volume and Issue: 173, P. 106614 - 106614

Published: June 28, 2024

Language: Английский

---

The role of TSC1 and TSC2 proteins in neuronal axons

Molecular Psychiatry, Journal Year: 2024, Volume and Issue: 29(4), P. 1165 - 1178

Published: Jan. 11, 2024

Language: Английский

---

Effects of three microtubule-associated proteins (MAP2, MAP4, and Tau) on microtubules’ physical properties and neurite morphology

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: May 31, 2023

Abstract The physical properties of cytoskeletal microtubules have a multifaceted effect on the expression their cellular functions. A superfamily microtubule-associated proteins, MAP2, MAP4, and tau, promote polymerization microtubules, stabilize formed affect microtubules. Here, we show differences in effects these three MAPs When microtubule-binding domain fragments MAP4 isoforms were added to vitro observed by fluorescence microscopy, tau-bound showed straighter morphology than bound MAP2 isoforms. Flexural rigidity was evaluated shape teardrop pattern when placed hydrodynamic flow, revealing that least flexible. full-length fused with EGFP expressed human neuroblastoma (SH-SY5Y) cells, apical regions protrusions expressing tau cells MAP4. On other hand, tau-expressing had fewest branches. These results suggest which are regulated MAPs, contribute morphogenesis neurites.

Language: Английский

---

A Multimodal Fitting Approach to Construct Single-Neuron Models with Patch Clamp and High-Density Microelectrode Arrays

Neural Computation, Journal Year: 2024, Volume and Issue: 36(7), P. 1286 - 1331

Published: May 22, 2024

In computational neuroscience, multicompartment models are among the most biophysically realistic representations of single neurons. Constructing such usually involves use patch-clamp technique to record somatic voltage signals under different experimental conditions. The data then used fit many parameters model. While patching soma is currently gold-standard approach build models, several studies have also evidenced a richness dynamics in dendritic and axonal sections. Recording from alone makes it hard observe correctly parameterize activity nonsomatic compartments. order provide richer set as input we here investigate combination recordings with high-density microelectrode arrays (HD-MEAs). HD-MEAs enable observation extracellular potentials neural neuronal compartments at subcellular resolution. this work, introduce novel framework combine HD-MEA construct models. We first validate our method on ground-truth model known show that features extracted signals, addition intracellular ones, yields enabling better fits than using alone. demonstrate procedure by constructing cell vitro cultures. proposed multimodal fitting has potential augment modeling efforts neuroscience community field more can be validated.

Language: Английский

---

Distinctive Properties and Powerful Neuromodulation of Nav1.6 Sodium Channels Regulates Neuronal Excitability

Cells, Journal Year: 2021, Volume and Issue: 10(7), P. 1595 - 1595

Published: June 25, 2021

Voltage-gated sodium channels (Navs) are critical determinants of cellular excitability. These ion exist as large heteromultimeric structures and their activity is tightly controlled. In neurons, the isoform Nav1.6 highly enriched at axon initial segment nodes, making it for initiation propagation neuronal impulses. Changes in expression function profoundly impact input-output properties neurons normal pathological conditions. While mutations may cause channel dysfunction, aberrant changes also be result complex modes regulation, including various protein-protein interactions post-translational modifications, which can alter membrane excitability firing properties. Despite decades research, complexities modulation health disease still being determined. some modulatory mechanisms have similar effects on other Nav isoforms, others isoform-specific. Additionally, considerable progress has been made toward understanding how individual protein and/or modifications affect function. However, there more to learned about these different interact. Here, we examine role provide a thorough review this channel's regulatory they contribute neuromodulation.

Language: Английский

---

Mitochondrial transplantation rescues neuronal cells from ferroptosis

Free Radical Biology and Medicine, Journal Year: 2023, Volume and Issue: 208, P. 62 - 72

Published: Aug. 1, 2023

Ferroptosis is a type of oxidative cell death that can occur in neurodegenerative diseases and involves damage to mitochondria. Previous studies demonstrated preventing mitochondrial dysfunction rescue cells from ferroptotic death. However, the complexity timing therapeutic interventions make it difficult develop an effective treatment strategy against ferroptosis neurodegeneration conditions. In this study, we explored use transplantation as novel approach for neuronal Our data showed isolated exogenous mitochondria were incorporated into both healthy immortalized hippocampal HT-22 primary cortical neurons (PCN). The incorporation was accompanied by increased metabolic activity survival through attenuating lipid peroxidation superoxide production. Further, function complexes I, III V activities contributed neuroprotective Similarly, have also internalization mouse PCN; these internalized found effectively preserve networks when challenged with stimuli. administration axonal compartment two-compartment microfluidic device induced transportation body, which prevented fragmentation network PCN. These findings suggest may be promising protecting

Language: Английский

---

Strikingly different neurotransmitter release strategies in dopaminergic subclasses

Published: Feb. 10, 2025

Neuronal function is intimately tied to axodendritic polarity. Neurotransmitter release, for example, usually the role of axon. There are widespread exceptions this rule, however, including many mammalian neuronal types that can release neurotransmitter from their dendrites. In mouse olfactory bulb, closely related subclasses dopaminergic interneuron differ markedly in polarity, with one subtype lacking an axon entirely. These axon-bearing and anaxonic have distinct developmental profiles sensory responses, but how fundamental polarity differences translate functional outputs remains entirely unknown. Here, we provide anatomical evidence strategies among these subtypes: cells dendrites, while neurons exclusively intermittently myelinated structural linked a clear distinction: anaxonic, not capable self-inhibition. Our findings suggest variations produce striking distinctions outputs, even may play separate roles information processing.

Language: Английский

---

Strikingly different neurotransmitter release strategies in dopaminergic subclasses

Published: Feb. 10, 2025

Neuronal function is intimately tied to axodendritic polarity. Neurotransmitter release, for example, usually the role of axon. There are widespread exceptions this rule, however, including many mammalian neuronal types that can release neurotransmitter from their dendrites. In mouse olfactory bulb, closely related subclasses dopaminergic interneuron differ markedly in polarity, with one subtype lacking an axon entirely. These axon-bearing and anaxonic have distinct developmental profiles sensory responses, but how fundamental polarity differences translate functional outputs remains entirely unknown. Here, we provide anatomical evidence strategies among these subtypes: cells dendrites, while neurons exclusively intermittently myelinated structural linked a clear distinction: anaxonic, not capable self-inhibition. Our findings suggest variations produce striking distinctions outputs, even may play separate roles information processing.

Language: Английский

---