Characterizing the gut microbiota composition in Taiwanese hypertensive patients using 16S rRNA sequencing analysis DOI Creative Commons
Ming‐Shan Chen,

Shin-Kuang Jiang,

Zhi-Yong Chong

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 18, 2023

Abstract Objective Hypertension (HTN) is a significant risk factor for cardiovascular and cerebrovascular diseases. Accumulating evidence suggests close relationship between HTN dysbiosis of the gut microbiota. Methods In present study, we recruited 23 patients 17 controls matched demographic characteristics. DNA extracted from fecal samples was subjected to Illumina MiSeq sequencing, targeting V3-V4 region bacterial 16S rRNA gene analysis. We compared diversity composition microbiota two groups. Results The α-diversity in similar that control group. β-diversity analysis showed slight differences microbial used Welch's t -test evaluate difference top 20 ASVs group group, results Tyzzerella significantly increased, while Faecalibacterium decreased also conducted Kruskal-Wallis test compare at various taxonomic levels (phylum, class, order, family, genus, species). revealed reduction abundance , Lachnospiraceae_UCG-004 Coprobacter Alphaproteobacteria Additionally, Linear Discriminant Analysis Effect Size (LEfSe) enrichment Enterobacteriaceae whereas Lachnospiraceae_UCG_004 dominated As exhibited lower according above results, performed PCR using -specific primers analyzed their through agarose gel electrophoresis, confirming reduced addition, Tax4Fun2 employed examine functionality Conclusion studied hypertensive population Taiwan found has patients. This unique alteration may provide insights into pathogenesis aid development novel biomarkers therapeutic targets.

Language: Английский

Glycosphingolipids: From metabolism to chemoenzymatic total synthesis DOI

Mitra K. F. Bonab,

Zhongwu Guo, Qingjiang Li

et al.

Organic & Biomolecular Chemistry, Journal Year: 2024, Volume and Issue: 22(33), P. 6665 - 6683

Published: Jan. 1, 2024

GSLs are the major glycolipids in vertebrates and mediate many key biological processes from intercellular recognition to

Language: Английский

Citations

2
Fundamental Neurochemistry Review: Lipids across microglial states DOI Creative Commons
Marianela E. Traetta, Haley A. Vecchiarelli, Marie‐Ève Tremblay

et al.

Journal of Neurochemistry, Journal Year: 2024, Volume and Issue: 169(1)

Published: Dec. 18, 2024

The capacity of immune cells to alter their function based on metabolism is the basis emerging field immunometabolism. Microglia are resident innate central nervous system, and it a current focus investigate how alterations in impact these cells. have ability utilize lipids, such as fatty acids, energy sources, but also lipids can microglial form function. Recent studies highlighting different states transcriptional signatures highlighted modifications lipid processing defining states. This review highlights recent uses altered pathways discuss understanding biology microglia. here may phagocytic functioning or pro- anti-inflammatory balance. These provide foundation by which supplementation diet could influence Furthermore, targets modulating new treatment avenues.

Language: Английский

Citations

2
New metabolic insights into the mechanism of ifosfamide encephalopathy DOI Open Access
Diren Beyoğlu, Paul Hamberg, Nikki S. IJzerman

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 182, P. 117773 - 117773

Published: Dec. 17, 2024

Language: Английский

Citations

1
The identification of novel stroke-related sphingolipid biomarkers using UPLC-MS/MS DOI

Zhi Yang,

Xuhui Deng,

Jinhua Zhu

et al.

Clinica Chimica Acta, Journal Year: 2023, Volume and Issue: 552, P. 117652 - 117652

Published: Nov. 17, 2023

Language: Английский

Citations

3
Long-term intake of thermo-induced oxidized oil results in anxiety-like and depression-like behaviors: involvement of microglia and astrocytes DOI

Meishan Lu,

Jiachen Shi, Xue Li

et al.

Food & Function, Journal Year: 2024, Volume and Issue: 15(8), P. 4037 - 4050

Published: Jan. 1, 2024

Frequent consumption of fried foods has been strongly associated with a higher risk anxiety and depression, particularly among young individuals.

Language: Английский

Citations

0
Circulating sphingolipids and subclinical brain pathology: the cardiovascular health study DOI Creative Commons
Kristine F. Moseholm, Jens W. Horn,

Annette L. Fitzpatrick

et al.

Frontiers in Neurology, Journal Year: 2024, Volume and Issue: 15

Published: May 3, 2024

Background Sphingolipids are implicated in neurodegeneration and neuroinflammation. We assessed the potential role of circulating ceramides sphingomyelins subclinical brain pathology by investigating their association with magnetic resonance imaging (MRI) measures biomarkers injury, neurofilament light chain (NfL) glial fibrillary acidic protein (GFAP) Cardiovascular Health Study (CHS), a large intensively phenotyped cohort older adults. Methods Brain MRI was offered twice to CHS participants mean 5 years between scans, results were available from both time points 2,116 (mean age 76 years; 40% male; 25% APOE ε4 allele carriers). measured 8 ceramide sphingomyelin species plasma samples examined associations several MRI, including worsening grades white matter hyperintensities ventricular size, number infarcts, atrophy subset quantitative measures. also investigated sphingolipid serum NfL GFAP. Results In fully adjusted model, higher levels long (16-carbon) saturated fatty acid associated blood [β = 0.05, false-discovery rate corrected P ( FDR ) 0.004 β 0.06, < 0.001, respectively]. contrast, very (20- 22-carbon) acids tended have an inverse NfL. secondary analyses, we found interaction d18:1/20:0 sex for <0.001), such that odds infarcts women [OR 1.26 (95%CI: 1.07, 1.49), 0.03]. did not observe any GFAP levels, grade, bilateral hippocampal volume, or total volume. Conclusion Overall, our comprehensive investigation supports evidence increased aging direction depends on attached sphingosine backbone.

Language: Английский

Citations

0
Elucidating of the metabolic impact of risperidone on brain microvascular endothelial cells using untargeted metabolomics-based LC-MS DOI Creative Commons

Surachai Ngamratanapaiboon,

Napatarin Srikornvit,

Patipol Hongthawonsiri

et al.

Toxicology Reports, Journal Year: 2024, Volume and Issue: 13, P. 101691 - 101691

Published: July 9, 2024

Risperidone is useful for the treatment of schizophrenia symptoms; however, it also has side effects, and an overdose can be harmful. The metabolic effects risperidone at high therapeutic doses its metabolites have not been elucidated. Endogenous cellular may comprehensively analyzed using untargeted metabolomics-based liquid chromatography-mass spectrometry (LC-MS), which reveal changes in cell regulation pathways. By identifying pathway a nontargeted LC-MS approach, we aimed to shed light on potential toxicological high-dose brain microvascular endothelial cells (MVECs) associated with human blood barrier. A total 42 were selected as significant putative response MVECs. Six highly correlated pathways identified, including those involving diacylglycerol, fatty acid, ceramide, glycerophospholipid, amino tricarboxylic acid metabolism. We demonstrated that methods focused metabolomics are used clarify mechanism drug-induced toxicity.

Language: Английский

Citations

0
MAMs and Mitochondrial Quality Control: Overview and Their Role in Alzheimer’s Disease DOI

Jian-Sheng Luo,

Wen-Hu Zhai,

Lingling Ding

et al.

Neurochemical Research, Journal Year: 2024, Volume and Issue: 49(10), P. 2682 - 2698

Published: July 13, 2024

Language: Английский

Citations

0
1Ceramide synthase 6 induces mitochondrial dysfunction and apoptosis in hemin-treated neurons by impairing mitophagy through interacting with sequestosome 1 DOI Creative Commons

Aoqian Xu,

Yikui Liu,

Baofeng Wang

et al.

Free Radical Biology and Medicine, Journal Year: 2024, Volume and Issue: 227, P. 282 - 295

Published: Dec. 4, 2024

Language: Английский

Citations

0
Lipidomics Analysis of Human HMC3 Microglial Cells in an In Vitro Model of Metabolic Syndrome DOI Creative Commons
Mateusz Chmielarz, Mariusz A. Bromke, Mateusz Olbromski

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(10), P. 1238 - 1238

Published: Sept. 30, 2024

Metabolic endotoxemia (ME) is associated with bacterial lipopolysaccharide (LPS, endotoxin) and increased levels of saturated fatty acids (SFAs) in the bloodstream, causing systemic inflammation. ME usually accompanies obesity a diet rich fats, especially SFAs. Numerous studies confirm effect ME-related endotoxin on microglial activation. Our study aimed to assess lipid metabolism immune response microglia pre-stimulated TNFα (Tumor Necrosis Factor α) then palmitic acid (PA). Using ELISA, we determined cytokines IL-1β, IL-10, IL-13 (interleukin-1β, -10, -13, TGFβ (Transforming Growth β) culture medium from cells stimulated for 24 h treated LPS (10 ng/mL) PA (200 µM) h. HMC3 (Human Microglial Cells clone 3) produced negligible amounts after stimulation but secreted moderate TGFβ. Changes accompanied changes TREM2 (Triggering Receptor Expressed Myeloid 2) expression. expression, while treatment decreased it. Endotoxin ceramide levels, triglyceride levels. These results indicated that pre-stimulation significantly affects its interactions modulates metabolism, which may lead activation silencing neurodegeneration.

Language: Английский

Citations

0