SenMayo transcriptomic senescence panel highlights glial cells in the ageing mouse and human retina DOI Creative Commons
Samyuktha Suresh, Gayathri Karthik, John F. Ouyang

et al.

npj Aging, Journal Year: 2024, Volume and Issue: 10(1)

Published: Nov. 30, 2024

There is a growing need to better characterise senescent cells in the CNS and retina. The recently published SenMayo gene panel was developed identify transcriptomic signatures of senescence across multiple organ systems, but retina not included. While other approaches have identified retina, these largely focused on experimental models young animals. We therefore conducted detailed single-cell RNA-seq analysis cell populations different aged mice compared with five comprehensive human mouse brain transcriptome datasets. Transcriptomic were most apparent retinal glial cells, IL4, 13 10 AP1 pathway being prominent markers involved. Similar levels transcriptional observed glia old mice, whereas showed significantly increased enrichment scores advancing age.

Language: Английский

Autophagy in dry AMD: A promising therapeutic strategy for retinal pigment epithelial cell damage DOI

Zhao Zhang,

Fengming Liang,

Jun Chang

et al.

Experimental Eye Research, Journal Year: 2024, Volume and Issue: 242, P. 109889 - 109889

Published: April 7, 2024

Language: Английский

Citations

6
Novel human iPSC models of neuroinflammation in neurodegenerative disease and regenerative medicine DOI Creative Commons

Rose Ana Summers,

Francesca Fagiani, David H. Rowitch

et al.

Trends in Immunology, Journal Year: 2024, Volume and Issue: 45(10), P. 799 - 813

Published: Sept. 21, 2024

Language: Английский

Citations

4
Identifying microglia-derived NFKBIA as a potential contributor to the pathogenesis of Alzheimer’s disease and age-related macular degeneration DOI
Shizhen Lei,

Yani Liu

Journal of Alzheimer s Disease, Journal Year: 2025, Volume and Issue: unknown

Published: March 19, 2025

Background Alzheimer's disease (AD) and age-related macular degeneration (AMD) place considerable health burden on affected individuals significant economic society. Objective This study aims to explore the shared cellular molecular mechanisms underlying pathogenesis of AD AMD. Methods The investigation in this is conducted via single-cell bulk tissue transcriptomic analysis. Transcriptomic datasets AMD were obtained from GEO database. differentially expressed genes (DEGs) control AD- AMD-affected samples identified. Functional enrichment analysis for DEGs was subsequently performed. Then, protein-protein interaction (PPI) network these established STRING database hub identified by Cytoscape software. Single-cell performed using Seurat R package their expression different cell types. Results Differential 127 two diseases, including 71 upregulated 56 downregulated genes. Upregulated enriched inflammation, gliogenesis, apoptosis, response bacterial viral infection mitochondrial function energy production. PPI determined 10 genes, which NFKBIA gene associated with severity both Moreover, showed that highly microglia disease-affected tissues. Conclusions findings indicated high important contributors progression Microglia-derived might serve as a potential therapeutic target

Language: Английский

Citations

0
Differential Effect of Aldosterone or Mineralocorticoid Receptor Overexpression on Retinal Inflammation DOI Creative Commons
Bastien Leclercq, Dan Mejlachowicz, Linxin Zhu

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2024, Volume and Issue: 65(12), P. 39 - 39

Published: Oct. 25, 2024

Overactivation of the mineralocorticoid receptor (MR) pathway is proinflammatory and contributes to pathogenesis diabetic retinopathy age-related macular degeneration. Excess aldosterone, specific MR ligand, known stimulate production cytokines chemokines in extrarenal tissues cells. In RPE/choroid complex, aldosterone upregulated genes encoding proteins inflammatory response downregulated involved synaptic activity neurotransmitters. Yet, cortisol, which main ligand eye, a potent anti-inflammatory endogenous glucocorticoid. The aim present work was better understand role activation retinal inflammation either by acute injection or overexpression receptor.

Language: Английский

Citations

1
The effects of time restricted feeding on age-related changes in the mouse retina DOI Creative Commons

Cade A. Huston,

Madison Milan,

Michaela L. Vance

et al.

Experimental Gerontology, Journal Year: 2024, Volume and Issue: 194, P. 112510 - 112510

Published: July 5, 2024

Dietary modifications such as caloric restriction (CR) and intermittent fasting (IF) have gained popularity due to their proven health benefits in aged populations. In time restricted feeding (TRF), a form of fasting, the amount for food intake is regulated without restricting intake. TRF beneficial central nervous system support brain context aging. Therefore, we here ask whether also exerts effects retina. We compared mice (24 months) on paradigm (access six hours per day) either 6 or 12 months against young control (8 an ad libitum diet. examined changes retina at functional (electroretinography), structural (histology fluorescein angiograms) molecular (gene expression) level. treatment showed amelioration age-related reductions both scotopic photopic b-wave amplitudes suggesting retinal interneuron signaling. did not affect signs inflammation microglial activation histological Our data indicate that helps preserve some aspects function are decreased with aging, adding our understanding altered patterns may confer.

Language: Английский

Citations

0
SenMayo transcriptomic senescence panel highlights glial cells in the ageing mouse and human retina DOI Creative Commons
Samyuktha Suresh, Gayathri Karthik, John F. Ouyang

et al.

npj Aging, Journal Year: 2024, Volume and Issue: 10(1)

Published: Nov. 30, 2024

There is a growing need to better characterise senescent cells in the CNS and retina. The recently published SenMayo gene panel was developed identify transcriptomic signatures of senescence across multiple organ systems, but retina not included. While other approaches have identified retina, these largely focused on experimental models young animals. We therefore conducted detailed single-cell RNA-seq analysis cell populations different aged mice compared with five comprehensive human mouse brain transcriptome datasets. Transcriptomic were most apparent retinal glial cells, IL4, 13 10 AP1 pathway being prominent markers involved. Similar levels transcriptional observed glia old mice, whereas showed significantly increased enrichment scores advancing age.

Language: Английский

Citations

0