Ascorbic acid supplementation in adolescent rats ameliorates anxiety‐like and depressive‐like manifestations of nicotine‐ethanol abstinence: Role of oxidative stress, inflammatory, and serotonergic mechanisms DOI

Alireza Najafzadeh,

M Mahdizadeh,

Samaneh Kakhki

et al.

International Journal of Developmental Neuroscience, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 4, 2024

Abstract Background The present study aims to assess the therapeutic potential of vitamin C (Vit C) on anxiety‐ and depressive‐like behavior induced by abstinence from chronic nicotine‐ethanol co‐exposure in adolescent male rats. Materials methods Adolescent rats were divided into seven experimental groups with ten as follows: 1) vehicle, 2) Nicotine (Nic)‐Ethanol (Eth): received Nic (2 mg/kg) Eth (20%) drinking water 21 42 days age, 3–5) Nic‐Eth‐Vit 100/200/400: age Vit 100/200/400 mg/kg 43 63 6) Nic‐Eth‐Bupropion (Bup)‐ Naloxone (Nal): Bup Nal 7) 400 mg/kg: age. Behavioral assessments done elevated plus maze (EPM), forced swimming test (FST), marble burring (MBT), open field tests (OFT). Furthermore, specific biochemical variables associated oxidative, inflammatory, serotonergic profiles quantified. Results According obtained results, anxiety depression treated We showed that two higher doses increases active struggling time FST decreases both spent peripheral zone OFT closed arms EPM. In addition, animals buried less number marbles MBT compared their control counterparts. oxidative stress inflammation cortical tissues Biochemical parameters improved Nic‐Eth group receiving 200/400 Bup‐Nal through establishing a balance between oxidant/anti‐oxidant inflammatory/anti‐inflammatory mediators. serotonin level was increased, while Monoamine oxidase (MAO) activity notably decreased. Conclusion findings support beneficial effect withdrawal various mechanisms such promotion antioxidant defense, suppression inflammatory mediators, enhancement serotoninergic function.

Language: Английский

Synergistic Neuroprotection Through Epigenetic Modulation by Combined Curcumin-Enriched Turmeric Extract and L-Ascorbic Acid in Oxidative Stress-Induced SH-SY5Y Cell Damage DOI Creative Commons
Jurairat Khongrum, Nootchanat Mairuae, Tongjit Thanchomnang

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(5), P. 892 - 892

Published: March 5, 2025

Epigenetic modulation plays a crucial role in neuroprotection by regulating cellular responses to stress, inflammation, and oxidative damage, particularly neurodegenerative diseases. Recognizing the therapeutic potential of epigenetic regulators, this study investigated synergistic neuroprotective effects curcumin-enriched turmeric extract combined with L-ascorbic acid, focusing on its pathways stress-induced neuronal damage. SH-SY5Y cells were treated combination at 20 40 µg/mL, subsequently exposed 200 µM hydrogen peroxide (H2O2) induce stress. Cell viability was assessed using MTT assay, while mechanisms evaluated analyzing markers modulation, apoptosis. The significantly enhanced cell viability, upregulated sirtuin-1 (SIRT1), reduced DNA methyltransferase 1 (DNMT1) expression, indicating effective modulation. Enhanced antioxidant defenses observed, as evidenced increased activities superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), along decreased malondialdehyde (MDA) reactive oxygen species (ROS) levels, alleviating Additionally, it suppressed nuclear factor kappa B (NF-κB) activity downstream mediator interleukin-6 (IL-6), thereby mitigating inflammation. treatment also anti-apoptotic Bcl-2 expression reducing pro-apoptotic markers, including caspase-3 caspase-9, suggesting inhibition intrinsic apoptotic pathway. These findings highlight novel combination, demonstrating ability modulate suppressing preventing undesired Its multifaceted properties make promising functional ingredient foods for disease intervention. However, further investigations, animal studies clinical trials, are essential evaluate safety potential.

Language: Английский

Citations

2
Role of Natural Compounds Modulating Heme Catabolic Pathway in Gut, Liver, Cardiovascular, and Brain Diseases DOI Creative Commons
Sri Jayanti, Libor Vı́tek, Camilla Dalla Verde

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(1), P. 63 - 63

Published: Jan. 2, 2024

The crucial physiological process of heme breakdown yields biliverdin (BV) and bilirubin (BR) as byproducts. BV, BR, the enzymes involved in their production (the “yellow players—YP”) are increasingly documented endogenous modulators human health. Mildly elevated serum concentration has been correlated with a reduced risk multiple chronic pro-oxidant pro-inflammatory diseases, especially elderly. BR BV per se have demonstrated to protect against neurodegenerative which oxygenase (HMOX), main enzyme pigments, is almost always altered. HMOX upregulation interpreted tentative defense ongoing pathologic mechanisms. With demonstration that cells possess YP, propensity be modulated, broad spectrum activity on signaling pathways, YP assumed role an adjustable system can promote health adults. Based that, there effort induce therapeutic option, natural compounds attractive alternative goal, possibly requiring only minimal changes life style. We review most recent evidence potential targeting context common condition adult elderly life.

Language: Английский

Citations

4
Focusing on Keap1, IKKβ, and Bcl2 proteins: predicted targets of stigmasterol in neurodegeneration DOI
Manoj Kumar Soni, Awadhesh Kumar, Rakesh Kumar

et al.

Journal of Receptors and Signal Transduction, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 12

Published: Feb. 13, 2025

Oxidative stress, driven by excess ROS, damages lipids, proteins, and DNA, leading to neuronal apoptosis inflammation, a key factor in neurodegenerative diseases. This study explored stigmasterol, bioactive phytosterol, with neuroprotective potential, revealing strong docking interactions, especially Keap1 (binding energy of −11.62 Kcal/mol). Stigmasterol formed two hydrogen bonds Ile258 Val305 Keap1, suggesting it could disrupt Keap1-Nrf2 potentially activating antioxidant responses promoting Nrf2 translocation the nucleus. In Bcl2-stigmasterol complex, which exhibited binding −8.41 Kcal/mol, hydrophobic interactions residues Ser50, Gln52, Leu185 stabilized indicating stigmasterol's role inhibiting strengthening Bcl2 mediated inhibition pro-apoptotic factors like Bax. Furthermore, IKKβ-stigmasterol complex displayed bond between Asp385 residue stigmasterol (2.83 Å), −8.33 suggested that may regulate inflammation stabilizing IKKβ, thereby preventing NF-κB reducing inflammation. Molecular dynamics simulations confirmed stability showed low RMSD values consistent bonding. RMSF Rg analyses indicated had effects on IKKβ. These results underscore potential for neuroprotection through anti-inflammatory actions.

Language: Английский

Citations

0
Benchmark doses (BMD) extrapolated from in vitro cytotoxicity experiments in SH-SY5Y cells using the EFSA Bayesian BMD web app: The study case of imidacloprid DOI
Lenin Ramírez-Cando, Santiago Ballaz

Computational Toxicology, Journal Year: 2025, Volume and Issue: unknown, P. 100346 - 100346

Published: April 1, 2025

Language: Английский

Citations

0
Pharmacotherapeutic potential of malvidin to cure imidacloprid induced hepatotoxicity via regulating PI3K/AKT, Nrf-2/Keap-1 and NF-κB pathway DOI
H. Matthew Edwards,

Warda Mustfa,

Shahaba Tehreem

et al.

Food and Chemical Toxicology, Journal Year: 2024, Volume and Issue: 190, P. 114816 - 114816

Published: June 14, 2024

Language: Английский

Citations

1
Alpha-Lipoic acid alleviates imidacloprid-induced neuro-behavioral deficits in rats via Nrf2/HO-1 pathway DOI

Mohit Nema,

Bhaskar Jyoti Dutta, Sanjiv Singh

et al.

Toxicology Mechanisms and Methods, Journal Year: 2023, Volume and Issue: 34(2), P. 176 - 188

Published: Oct. 30, 2023

Imidacloprid (IMI), a widely used pesticide in agriculture and potential food contaminant, poses significant health concerns. This study sought to comprehensively evaluate its neurotoxic effects while investigating the protective role of alpha-lipoic acid (ALA), naturally occurring dietary antioxidant renowned for capacity combat oxidative stress, support cardiovascular health, maintain optimal nerve function. In this study, 28 rats were divided evenly into four groups administered oral treatments corn oil, IMI, IMI + ALA, respectively. The results indicated that exposed exhibited neurobehavioral impairments, decreased levels enzymes acetylcholinesterase activity, reduced expression HO-1 Nrf2, increased pro-inflammatory cytokines like IL-6 TNF-α their hippocampal tissues. Furthermore, histopathological analysis brain tissues, specifically cortex hippocampus, from IMI-treated group revealed varying degrees neuronal degeneration. contrast, co-administered ALA alongside showed noticeable improvements all assessed toxicological parameters. underscores vital significance as therapeutic adjunct mitigating adverse consequences insecticide exposure. By harnessing Nrf2/HO-1 pathway, demonstrates ability shield against IMI-induced neurotoxicity, offering promising avenue enhancing public safety. As result, our findings advocate incorporation daily supplement fortify resilience stress-related deficits linked exposure, thereby advancing understanding neuroprotection strategies face environmental challenges.

Language: Английский

Citations

1
Imidacloprid unique and repeated treatment produces cholinergic transmission disruption and apoptotic cell death in SN56 cells. DOI Creative Commons
Paula Moyano, Andrea Flores,

Javier San Juan

et al.

Food and Chemical Toxicology, Journal Year: 2024, Volume and Issue: 193, P. 114988 - 114988

Published: Sept. 7, 2024

Language: Английский

Citations

0
Ascorbic acid supplementation in adolescent rats ameliorates anxiety‐like and depressive‐like manifestations of nicotine‐ethanol abstinence: Role of oxidative stress, inflammatory, and serotonergic mechanisms DOI

Alireza Najafzadeh,

M Mahdizadeh,

Samaneh Kakhki

et al.

International Journal of Developmental Neuroscience, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 4, 2024

Abstract Background The present study aims to assess the therapeutic potential of vitamin C (Vit C) on anxiety‐ and depressive‐like behavior induced by abstinence from chronic nicotine‐ethanol co‐exposure in adolescent male rats. Materials methods Adolescent rats were divided into seven experimental groups with ten as follows: 1) vehicle, 2) Nicotine (Nic)‐Ethanol (Eth): received Nic (2 mg/kg) Eth (20%) drinking water 21 42 days age, 3–5) Nic‐Eth‐Vit 100/200/400: age Vit 100/200/400 mg/kg 43 63 6) Nic‐Eth‐Bupropion (Bup)‐ Naloxone (Nal): Bup Nal 7) 400 mg/kg: age. Behavioral assessments done elevated plus maze (EPM), forced swimming test (FST), marble burring (MBT), open field tests (OFT). Furthermore, specific biochemical variables associated oxidative, inflammatory, serotonergic profiles quantified. Results According obtained results, anxiety depression treated We showed that two higher doses increases active struggling time FST decreases both spent peripheral zone OFT closed arms EPM. In addition, animals buried less number marbles MBT compared their control counterparts. oxidative stress inflammation cortical tissues Biochemical parameters improved Nic‐Eth group receiving 200/400 Bup‐Nal through establishing a balance between oxidant/anti‐oxidant inflammatory/anti‐inflammatory mediators. serotonin level was increased, while Monoamine oxidase (MAO) activity notably decreased. Conclusion findings support beneficial effect withdrawal various mechanisms such promotion antioxidant defense, suppression inflammatory mediators, enhancement serotoninergic function.

Language: Английский

Citations

0