Metabolite Biomarkers for Early Ischemic Hypoxic Encephalopathy: An Experimental Study Using the NeoBase 2 MSMS Kit in a Rat Model

Published: Dec. 5, 2023

Hypoxic-ischemic encephalopathy (HIE) is one of the most common causes childhood disability. Hypothermic therapy currently only approved neuroprotective approach. However, early diagnosis HIE can be challenging, especially in first hours after birth when decision to treat with hypothermic critical. Differentiating from other neonatal conditions, such as sepsis, further complicates diagnosis. This study investigated utility a metabolomic-based approach using NeoBase 2 MSMS kit diagnose dry blood stains Rice-Vannucci model rats. We evaluated diagnostic accuracy this method between 3 and 6 onset HIE, including context systemic inflammation concomitant therapy. Discriminant analysis revealed several metabolite patterns associated HIE. A logistic regression glycine levels achieved high areas under curve (AUC) 0.94 at 0.96 In addition, orthogonal partial least squares discriminant analysis, which included five metabolites, 100% sensitivity 80% specificity within These results highlight significant potential for could improve patient management outcomes serious illness.

Language: Английский

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β -estradiol alleviates Hypoxic-ischemic brain damage in neonatal rats through the GPER1 regulating AKT/NF-κB signal pathway

Archives of Medical Science, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 6, 2024

Introduction Research has established that estradiol (E2) offers neuroprotection against hypoxic-ischemic brain damage (HIBD) in neonatal rats, yet the underlying mechanisms are not fully understood. This study seeks to delineate whether E2's neuroprotective effects HIBD mediated through astrocytes by modulating G Protein-Coupled Estrogen Receptor 1 (GPER1) receptor and subsequent AKT Serine (AKT)/NF-κB signaling cascade. Material methods We developed an vivo model rats primary cultures of subjected oxygen-glucose deprivation-reoxygenation (OGD-R) as vitro model. E2 GPER1 inhibitor (G15) were administered according experimental design. Protein expression levels GPER1, phosphorylated (p-AKT), NF-κB p65, cleaved-caspase3 examined using Western blot analysis. Apoptosis was assessed via TUNEL assay, presence TNF-α IL-1β cell supernatant quantified ELISA. The localization p-AKT p65 determined immunofluorescence. Results Our findings indicate treatment significantly reduced volume infarction astrocyte apoptosis. upregulated while downregulating post-HIBD. Additionally, diminished secretion supernatant. G15 notably reversed associated molecular changes. Conclusions These results suggest may exert with inhibiting apoptosis p-AKT, NF-κB, thereby providing a potential therapeutic strategy for HIBD.

Language: Английский

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Role of pentoxifylline in neonatal hypoxic ischaemic encephalopathy: a systematic review of animal studies

Laboratory Animal Research, Journal Year: 2024, Volume and Issue: 40(1)

Published: Nov. 28, 2024

Abstract We systematically reviewed the evidence from animal studies assessing effects of pentoxifylline on neonatal hypoxic-ischemic encephalopathy (HIE). The PubMed, EMBASE, EMCARE, MEDLINE, Cochrane Library, and Google Scholar databases were searched for randomized quasi controlled trials (RCTs) in December 2023 to determine models HIE. quality included was assessed via SYRCLE risk bias (ROB) tool. certainty GRADE methodology. All seven (n = 248) involved a rat HIE model which (25–150 mg/kg) administered intraperitoneally. majority had unclear ROB. reported protective effect HIE-induced organ injury. Mortality comparable at doses between 25 75 mg/kg but higher 150 than control group. Three macroscopic changes HIE-affected organs. There significant reduction cerebral infarction (40 mg/kg), hippocampal atrophy, visible gut injury (60 mg/kg). A significantly lower number Caspase 3 immunoreactive cells necrotic observed 60 dose, whereas 100 dose deleterious effect. other reduced levels proinflammatory markers including IL-6 TNF-alpha. Current (with low uncertainty) suggests that has potential improve mortality attenuate following Adequately powered, well-designed human RCTs are needed confirm our findings.

Language: Английский

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Advanced Trans-EEGNet Deep Learning Model for Hypoxic-Ischemic Encephalopathy Severity Grading

Mathematics, Journal Year: 2024, Volume and Issue: 12(24), P. 3915 - 3915

Published: Dec. 12, 2024

Hypoxic-ischemic encephalopathy (HIE) is a brain injury condition that poses significant risk to newborns, potentially causing varying degrees of damage the central nervous system. Its clinical manifestations include respiratory distress, cardiac dysfunction, hypotension, muscle weakness, seizures, and coma. As HIE represents progressive injury, early identification extent implementation appropriate treatment are crucial for reducing mortality improving outcomes. patients may face long-term complications such as cerebral palsy, epilepsy, vision loss, developmental delays. Therefore, prompt hypoxic-ischemic symptoms can help reduce severe sequelae in patients. Currently, hypothermia therapy one most effective treatments However, not all newborns with suitable this therapy, making rapid accurate assessment critical treatment. Among patients, has shown better efficacy those diagnosed moderate within 6 h birth, establishing time frame golden period During period, an severity essential formulating strategies predicting outcomes affected infants. This study proposes method addressing data imbalance noise interference through preprocessing techniques, including filtering SMOTE. It then employs EEGNet, deep learning model specifically designed EEG classification, combined Transformer featuring attention mechanism excels at capturing sequential features construct Trans-EEGNet model. outperforms previous methods computation feature extraction, enabling classification newborns.

Language: Английский

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Clinical and laboratory study of premature infants with hypoxic-ischemic encephalopathy

International Journal of Science and Research Archive, Journal Year: 2023, Volume and Issue: 9(2), P. 920 - 925

Published: Aug. 30, 2023

We conducted a comprehensive clinical and laboratory study of 102 preterm infants with hypoxic-ischemic encephalopathy (HIE) varying gestational ages. All were born to mothers complicated obstetric history. Clinical manifestation CNS injury was observed in 100% this cohort. Intracranial hemorrhages (ICH) grades I-IV registered 38 (37.2%) cases, while periventricular leukomalacia (PVL) present 5 (4.9%) cases. Intrauterine growth restriction (IUGR) 36 (35.3%) respiratory distress syndrome (RDS) 65 (63.7%) Mild cerebral ischemia diagnosed 51 (50%) moderate-severity cases 32 (31.4%) severe CI 19 (18.6%) infants. The severity progression HIE shown be dependent on age, intrauterine hypoxia, birth asphyxia. health status their plays crucial role the development other systemic injuries Therefore, premature births remain pertinent issue modern society.

Language: Английский

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