Characterising cerebrovascular reactivity and the pupillary light response–a comparative study DOI Creative Commons
Sierra Sparks, Genevieve Hayes, Joana Pinto

et al.

Frontiers in Physiology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 8, 2024

Introduction Smooth muscle is integral to multiple autonomic systems, including cerebrovascular dynamics through vascular smooth cells and in ocular dynamics, by regulating pupil size. In the brain, function plays a role reactivity (CVR) that describes changes blood vessel calibre response vasoactive stimuli. Similarly, size regulation can be measured using pupillary light (PLR), pupil’s reaction levels. The primary aim of this study was explore interplay between cerebral flow evaluated CVR PLR, respectively. Methods A total 20 healthy adults took part gas stimulus protocol dark flash PLR protocol. calculated as velocity change middle artery, transcranial Doppler ultrasound 5% increase CO 2 . Multiple metrics were with clinical pupillometer. Results all within expected physiological ranges for adults. Nine different metrics, assessed protocols, compared against CVR. significant negative relationship observed latency No statistically relationships found other metrics. Conclusion This first investigate dynamics. observed. Future work includes evaluating these more robust measurement techniques larger, diverse cohort. Notably, research warranted into its connection function.

Language: Английский

Low-cost physiology and behavioral monitor for intravital imaging in small mammals DOI Creative Commons
Yuntao Li, Alfredo Cárdenas‐Rivera, Chang Liu

et al.

Neurophotonics, Journal Year: 2025, Volume and Issue: 12(01)

Published: Jan. 25, 2025

SignificanceFunctional brain imaging experiments in awake animals require meticulous monitoring of animal behavior to screen for spontaneous behavioral events. Although these events occur naturally, they can alter cell signaling and hemodynamic activity the confound functional measurements.AimWe developed a centralized, user-friendly, stand-alone platform that includes an fixation frame, compact peripheral sensors, portable data acquisition system. The affordable, integrated benefit by motion detection alertness levels as complementary readouts measurements.ApproachA custom system was designed using powerful, inexpensive microcomputer. We customized accelerometer miniature camera modules efficient, real-time pupil diameter. then tested validated platform's performance with optical intrinsic signal GCaMP fluorescence calcium activation mice.ResultsThe shows promise detecting dilation while imaging. Stimulus-induced found initiate earlier than cortical hemodynamics slower rise time. Compared neuronal response, stimulus-induced initiated later time.ConclusionsWe monitor dynamics. device be easily coupled synchronized systems behavior, alertness, studies.

Language: Английский

Citations

0
Broad repetitive transcranial magnetic stimulation (rTMS) of the precuneus in Alzheimer's disease: A rationale and study design DOI Creative Commons
Michael K. Leuchter, Hanadi Ajam Oughli, Kelly A. Durbin

et al.

Alzheimer s & Dementia Translational Research & Clinical Interventions, Journal Year: 2025, Volume and Issue: 11(1)

Published: Jan. 1, 2025

Language: Английский

Citations

0
Characterising cerebrovascular reactivity and the pupillary light response–a comparative study DOI Creative Commons
Sierra Sparks, Genevieve Hayes, Joana Pinto

et al.

Frontiers in Physiology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 8, 2024

Introduction Smooth muscle is integral to multiple autonomic systems, including cerebrovascular dynamics through vascular smooth cells and in ocular dynamics, by regulating pupil size. In the brain, function plays a role reactivity (CVR) that describes changes blood vessel calibre response vasoactive stimuli. Similarly, size regulation can be measured using pupillary light (PLR), pupil’s reaction levels. The primary aim of this study was explore interplay between cerebral flow evaluated CVR PLR, respectively. Methods A total 20 healthy adults took part gas stimulus protocol dark flash PLR protocol. calculated as velocity change middle artery, transcranial Doppler ultrasound 5% increase CO 2 . Multiple metrics were with clinical pupillometer. Results all within expected physiological ranges for adults. Nine different metrics, assessed protocols, compared against CVR. significant negative relationship observed latency No statistically relationships found other metrics. Conclusion This first investigate dynamics. observed. Future work includes evaluating these more robust measurement techniques larger, diverse cohort. Notably, research warranted into its connection function.

Language: Английский

Citations

1