MicroRNAs Associated with Parenchymal Hematoma After Endovascular Mechanical Reperfusion for Acute Ischemic Stroke in Rats

Biomedicines, Journal Year: 2025, Volume and Issue: 13(2), P. 449 - 449

Published: Feb. 12, 2025

Background/Objectives: Hemorrhagic transformation after endovascular thrombectomy predicts poor outcomes in acute ischemic stroke with large-vessel occlusion. The roles of microRNAs (miRNAs) the pathogenesis parenchymal hematoma (PH) still remain unclear. This study aimed to investigate miRNA and mRNA regulatory network associated PH mechanical reperfusion an animal model oxygen-glucose deprivation/reoxygenation (OGD/R) model. Methods: Twenty-five miRNAs were assessed a reperfusion-induced hemorrhage rats under hyperglycemic conditions receiving 5 h middle cerebral artery differentially expressed neuron, astrocyte, microglia, brain microvascular endothelial cell (BMEC), pericyte OGD/R. predicted target genes further miRNA-mRNA was established. Results: Thirteen down-regulated (miRNA-29a-5p, miRNA-29c-3p, miRNA-126a-5p, miRNA-132-3p, miRNA-136-3p, miRNA-142-3p, miRNA-153-5p, miRNA-218a-5p, miRNA-219a-2-3p, miRNA-369-5p, miRNA-376a-5p, miRNA-376b-5p, miRNA-383-5p) one up-regulated (miRNA-195-3p) found rat peri-infarct reperfusion. Of these 14 PH-related miRNAs, 10 significantly at least two five BMEC, models OGD/R, consistent results. Thirty-one hub Forty-nine axes revealed, they related mechanisms inflammation, immunity, oxidative stress, apoptosis. Conclusions: Fourteen OGD/R models. Simultaneously several cells neurovascular unit may serve as valuable targets for stroke.

Language: Английский

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Expression of S100β during mouse cochlear development

European Journal of Histochemistry, Journal Year: 2025, Volume and Issue: 69(1)

Published: March 10, 2025

In the present study, expression of S100β was examined in mouse cochlea from embryonic day 17 (E17) to postnatal 32 (P32) using immunofluorescence, aiming explore its possible role auditory system. At E17, not detected, except external cochlear wall. Starting at E18.5, staining appeared organ Corti and stria vascularis. E18.5 P1 Corti, confined developing pillar cells. By P6, cytoplasmic evident inner outer cells, forming tunnel Corti. Additionally, extended medially into three rows Deiter’s with labeling their phalangeal processes. P8, continued be expressed heads, bodies, feet two as well soma processes lateral wall P8 cochlea, only vascularis but also spiral ligament. Between P10 P12, maintained cells wall, limbus. From P14 onwards, ceased vascularis, though it persisted ligament limbus adulthood. Within P21 remained immunostaining observed specifically cell cups P21. adult (P28 P32), declined both The dynamic spatiotemporal changes during ontogeny suggest development hearing function.

Language: Английский

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MicroRNAs and mRNA Regulatory Network of Parenchymal Hematoma after Endovascular Mechanical Reperfusion for Acute Ischemic Stroke in Rat

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 1, 2024

Abstract Hemorrhagic transformation after endovascular thrombectomy predicts poor outcomes in acute ischemic stroke with large vessel occlusion. The roles of microRNAs the pathogenesis parenchymal hematoma (PH) are still unclear. This study aims to investigate microRNA and mRNA regulatory network associated PH mechanical reperfusion animal model oxygen-glucose deprivation/reoxygenation (OGD/R) model. Twenty-five were assessed reperfusion-induced hemorrhage rats hyperglycemic conditions receiving 5-hour middle cerebral artery Thirteen down-regulated (miRNA-29a-5p, miRNA-29c-3p, miRNA-126a-5p, miRNA-132-3p, miRNA-136-3p, miRNA-142-3p, miRNA-153-5p, miRNA-218a-5p, miRNA-219a-2-3p, miRNA-369-5p, miRNA-376a-5p, miRNA-376b-5p, miRNA-383-5p) one up-regulated (miRNA-195-3p) found rat peri-infarct PH. Ten these 14 PH-related significantly differentially expressed at least two five models neuron, astrocyte, microglia, BMEC, pericyte OGD/R, consistent results. Thirty-one predicted hub target genes Forty-nine microRNA-mRNA axes revealed, which related mechanisms oxidative stress, apoptosis, immune, inflammation. Simultaneously several cells neurovascular unit may serve as valuable targets for stroke.

Language: Английский

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A Combined Extract Derived from Black Sticky Rice and Dill Improves Clinical Symptoms and Ischemic Stroke Biomarkers in Transient Ischemic Attack and Ischemic Stroke Patients

Nutrients, Journal Year: 2024, Volume and Issue: 16(22), P. 3946 - 3946

Published: Nov. 19, 2024

Currently, the adjuvant therapy to optimize restorative process after stroke is required due unsatisfied therapeutic efficacy. A combined extract of black sticky rice and dill showed potential in preclinical state, so we hypothesized that it could provide clinical benefits. three-arm, randomized, placebo-controlled study was set up elucidate this issue. Both males females (18-80 years old) who had experienced transient ischemic attacks or strokes within last 5-10 days with an NIHSS score ≤ 7 received standard treatment were randomly assigned receive either a placebo capsule containing at dose 600 1200 mg per day. The safety parameters, movement control, degree disability assessed 1, 2, 6 weeks intervention, serum biomarkers mentioned time points, except 2 weeks. After week high-dose (1200 mg/day) group improved NIHSSS, VCAM1, MMP-9. S100β VCAM1 also 6, while low-dose (600 only exhibited VCAM1. Therefore, high developed supplement improves recovery by improving motor impairment reducing endothelial dysfunction inflammation.

Language: Английский

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Predictive Value of Epicardial Adipose Tissue for Hemorrhagic Transformation and Functional Outcomes in Acute Ischemic Stroke Patients Undergoing Intravenous Thrombolysis Therapy

Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 11915 - 11929

Published: Dec. 1, 2024

Hemorrhagic transformation (HT) is a severe complication in patients with acute ischemic stroke (AIS) undergoing intravenous thrombolysis therapy (IVT). Epicardial adipose tissue (EAT) contributes to the development of AIS and disruption blood-brain barrier. This study aims investigate relationship between EAT risk HT, as well functional outcomes, treated IVT. 230 were included. volume (EATV) attenuation measured from chest CT scans. Follow-up cranial or magnetic resonance imaging (MRI) assessed HT occurrence. Patients stratified into groups based on presence parenchymal hematoma (PH), their 90-day outcomes (evaluated by modified Rankin Scale). occurred 52 (22.61%) patients, including 28 (12.17%) PH, 85 (37.00%) had poor prognosis. Compared first quartile EATV, third (OR 9.254, 95% CI 1.533-55.853) fourth 11.117, 1.925-64.211) EATV independent predictors HT; continuous variable 1.022, 1.005-1.040) was an factor for PH. Higher independently associated prognosis 1.170, 1.056-1.297). The area under curve predicting PH outcome 0.705 (95% 0.632-0.778), 0.693 0.597-0.789), 0.720 0.653-0.787). demonstrates that

Language: Английский

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