Synchronous Enhancement of Astaxanthin and Lipid Accumulation in Haematococcus Pluvialis Through Co-Mutation of Ethanol and Artp: An Exploration of Characteristics and Underlying Mechanisms DOI

Ma Yihua,

Xin Sun,

Youreng Sun

et al.

Published: Jan. 1, 2023

Haematococcus pluvialis is a precious algal species renowned for its ability to simultaneous production of astaxanthin and lipids. However, slow growth rate necessitates the development appropriate mutagenesis methodologies effectively enhance synchronous both This study introduced implemented co-mutation method Atmospheric Room Temperature Plasma(ARTP) ethanol. The process, performance, preliminary mechanisms underlying combined accumulation lipids in under influence ethanol combination with ARTP-induced mutation were compared analyzed. Findings showed that lipid contents relative total cell mass 110-2 strain reached 54.37%, surpassing 0-3 control by 16.98% 47.58% respectively. transcriptome-level analysis provided further insights into how ARTP induction could promote expressions carotenoid synthesis genes, thereby augmenting related enzymatic activities.

Language: Английский

Identification of CrDCL1-mediated microRNA biogenesis in green alga Chlamydomonas reinhardtii DOI Creative Commons
Ting Sun, Min Tao,

Qinglang Di

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 27, 2025

In eukaryotes, microRNAs (miRNAs) are generated by Dicer/Dicer-Like (DCL)-mediated cleavage. Previous studies identified three DCL genes (CrDCL1-3) in Chlamydomonas reinhardtii and indicated that CrDCL3 mediated the production of most miRNAs, while CrDCL1 protein was mainly involved siRNA biogenesis. The role miRNA biogenesis remains unclear. This study constructed a phylogenetic tree, performed structural analyses Dicer/DCL proteins from multiple species screened verified dcl1 dcl3 mutant strains. Using CC-5325 as control groups, we sRNA-sequencing, RT-qPCR, Northern blot verification experiments on to explore involvement C. reinhardtii. results demonstrated 22 including cre-miR910, novel-miR01, novel-miR03, novel-miR04, novel-miR05, novel-miR06, whose depended not only but also CrDCL3. present findings highlight some miRNAs may be CrDCL proteins, which is different animals plants. this will enrich knowledge eukaryotes.

Language: Английский

Citations

1
Engineering of ketocarotenoid biosynthetic pathway in Chlamydomonas reinhardtii through exogenous gene expression DOI
Arti Sharma, Prachi Nawkarkar,

Vikas U. Kapase

et al.

Systems Microbiology and Biomanufacturing, Journal Year: 2024, Volume and Issue: 4(3), P. 983 - 995

Published: March 20, 2024

Language: Английский

Citations

5
Performance evaluation of genetically modified microalgae in photosynthetic microbial fuel cells for carotenoids and power generation DOI
Arti Sharma,

Meenu Chhabra,

Shashi Kumar

et al.

Journal of environmental chemical engineering, Journal Year: 2024, Volume and Issue: 12(3), P. 112751 - 112751

Published: April 8, 2024

Language: Английский

Citations

5
Turning the industrially relevant marine alga Nannochloropsis red: one move for multifaceted benefits DOI
Meijing Liu, Lihua Yu, Jie Zheng

et al.

New Phytologist, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

Summary Nannochloropsis oceanica is an industrially relevant marine microalga rich in eicosapentaenoic acid (EPA, a valuable ω‐3 polyunsaturated fatty acid), yet the algal production potential remains to be unlocked. Here we engineered N. synthesize high‐value carotenoid astaxanthin independent of high‐light (HL) induction for achieving multifaceted benefits. By screening β‐carotenoid ketolases and hydroxylases various origins, strategically manipulating compartmentalization, fusion patterns, linkers enzyme pair, remarkable 133‐fold increase content was achieved . Iterative metabolic engineering efforts led further increases synthesis up 7.3 mg g −1 , highest reported microalgae under nonstress conditions. Astaxanthin found photosystem components allowed alga HL resistance augmented EPA production. Besides, co‐production by through fed‐batch cultivation approach. Our findings unveil untapped as robust, light‐driven chassis constitutive provide feasible strategies concurrent multiple biochemicals from CO 2 thereby paving way sustainable biotechnological applications this alga.

Language: Английский

Citations

4
Synchronous enhancement of astaxanthin and lipid accumulation in Haematococcus lacustris through co-mutation of ethanol and atmospheric and room temperature plasma: Exploration of characteristics and underlying mechanisms DOI
Yihua Ma, Xin Sun,

Youreng Sun

et al.

Bioresource Technology, Journal Year: 2024, Volume and Issue: 394, P. 130305 - 130305

Published: Jan. 8, 2024

Language: Английский

Citations

3
Establishment of a Generalizable Industrial Crop Model for Microwave Extraction of Unsaturated Fatty Acids DOI Creative Commons
Junyi Chen,

Didi Lu,

Shiqiang Chen

et al.

International Journal of Analytical Chemistry, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

To explore the relationship between unsaturated fatty acid (UFA) content and parameters for microwave extraction, multimaterial multiparameter testing was conducted in which five kinds of materials with different UFA contents (potato, wheat, corn, soybean, peanut) were selected experiment. Four factors, namely, extraction temperature ( X 1 ), time 2 proportional volume acetone 3 liquid‐to‐solid ratio 4 screened their significant effects by using Prob > | T values from Plackett–Burman A orthogonal experiment four factors levels designed separately Design‐Expert 8.05 software them concentrated. Microwave‐accelerated methyl esterification then performed, determined via gas chromatography (flame ionization detector). The optimal conditions , ) potato 80.68°C, 10.74 min, 0.80, 3.25 mL × g −1 1.08%; wheat: 80.81°C, 10.54 20.91 2.26%; corn: 81.18°C, 9.93 50.94 6.89%; soybean: 82.07°C, 9.07 93.87 15.81%; peanut: 83.12°C, 8.11 168.70 33.07%. Then, optimization results synthesized Origin 8.0 software, fitting degree cubic model highest, determination coefficients 0.9984, 0.9991, 0.8953, 0.9989, residual sums squares 0.2888, 0.1587, 0.8265, 0.1864, correlation are ideal. stability accuracy verified rice, coefficient predicted value actual 0.9998. This study systematically collates crops perspective multiparameter, can provide a large amount detailed basic data technology.

Language: Английский

Citations

0
Metabolic engineering of Mucor circinelloides to improve astaxanthin production DOI
Tahira Naz, Tariq Saeed, Samee Ullah

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 40(12)

Published: Nov. 2, 2024

Language: Английский

Citations

0
Synchronous Enhancement of Astaxanthin and Lipid Accumulation in Haematococcus Pluvialis Through Co-Mutation of Ethanol and Artp: An Exploration of Characteristics and Underlying Mechanisms DOI

Ma Yihua,

Xin Sun,

Youreng Sun

et al.

Published: Jan. 1, 2023

Haematococcus pluvialis is a precious algal species renowned for its ability to simultaneous production of astaxanthin and lipids. However, slow growth rate necessitates the development appropriate mutagenesis methodologies effectively enhance synchronous both This study introduced implemented co-mutation method Atmospheric Room Temperature Plasma(ARTP) ethanol. The process, performance, preliminary mechanisms underlying combined accumulation lipids in under influence ethanol combination with ARTP-induced mutation were compared analyzed. Findings showed that lipid contents relative total cell mass 110-2 strain reached 54.37%, surpassing 0-3 control by 16.98% 47.58% respectively. transcriptome-level analysis provided further insights into how ARTP induction could promote expressions carotenoid synthesis genes, thereby augmenting related enzymatic activities.

Language: Английский

Citations

0