Frontiers in Microbiology,
Journal Year:
2024,
Volume and Issue:
15
Published: July 3, 2024
To
exert
their
beneficial
effects,
microorganisms
used
in
live
bacteria-containing
products
must
be
viable
and
present
certain
amounts.
In
this
study,
we
developed
a
viability
assay
based
on
quantitative
PCR
coupled
with
propidium
monoazide
for
the
identification
enumeration
of
Lactobacillus
acidophilus
Bifidobacterium
bifidum
.
order
to
optimize
protocol,
thermal
inactivation
conditions
two
target
PMA
concentration
inhibiting
DNA
amplification
from
dead
cells
while
allowing
it
were
first
determined.
The
viability-PCR
protocol
was
then
applied
analyze
commercial
product
containing
microorganisms.
quantities
both
determined
using
tested
significantly
higher
than
those
obtained
standard
plate
count,
suggesting
presence
bacteria
but
non-culturable
physiological
state.
Moreover,
lower
amounts
detected
compared
achieved
PCR,
possibly
because
samples.
Our
results
suggest
that
method
proposed
here
is
suitable
alternative
rapid
accurate
quantification
assessment
L.
B.
could
easily
adopted
quality
control
screening
products.
Revista Brasileira Multidisciplinar,
Journal Year:
2024,
Volume and Issue:
27(1), P. 169 - 189
Published: Jan. 1, 2024
Em
2030,
1,12
milhões
de
pessoas
no
mundo
serão
obesas,
gerando
sérias
preocupações
saúde
global.
A
obesidade
não
se
limita
a
questões
estéticas,
e
está
intrinsecamente
ligada
distúrbios
metabólicos.
Embora
intervenções
farmacológicas,
dietéticas,
estilo
vida
educacionais
tenham
mostrado
eficazes
na
prevenção
tratamento
da
obesidade,
há
uma
necessidade
crescente
identificar
alternativas
baixo
custo
risco
que
possam
ser
adotadas
sem
efeitos
colaterais
prejudiciais.
Nesse
contexto,
modulação
microbiota
intestinal
emerge
como
área
promissora.
desempenha
um
papel
crucial
em
sistemas
o
cardiovascular,
imunológico,
endócrino,
inflamatório,
metabolismo
regulação
genética.
Nos
últimos
anos,
tem
havido
aumento
nas
evidências
desequilíbrio
relacionado
à
obesidade.
Assim,
essa
revisão
examina
os
probióticos
simbióticos
podem
ajudar
tratar
prevenir
destacando
possíveis
mecanismos
entre
binômio
microbiota-hospedeiro
seus
com
ou
desenvolvê-la.
Foi
realizada
busca
bases
dados:
Pubmed/MEDLINE,
ScienceDirect
SciELO.
Os
resultados
permitiram
estabelecer
panorama
dos
usos
terapêuticos
indivíduos
obesos
outras
patologias,
assim
hipóteses
correlações
as
alterações
obesogênica
nos
indivíduos.
Pode-se
concluir
tais
são
promissoras
capazes
modular
maneira
local
sistêmica
metabolismo,
parâmetros
bioquímicos
composição
corporal
alterados
pela
intervenção
complementar
alternativa
para
patologia.
Indonesian Journal of Applied Research (IJAR),
Journal Year:
2024,
Volume and Issue:
5(2), P. 134 - 146
Published: Aug. 29, 2024
Lactobacillus
casei
bacterium
in
probiotic
products
and
the
human
digestive
tract
could
be
decreased
so
that
encapsulation
preserved
techniques
need
to
protect
viability
of
casei.
The
aimed
this
study
was
determine
ability
survival
L.casei
with
cryoprotectant
materials
consist
a
combination
whey
sucrose
maltodextrin
using
freeze
drying
technique.
design
used
complete
randomized
consisting
five
treatments
two
replications
which
were
control;
10%
10%;
20%
10%.
anova
test
α
=
0.05
conducted,
if
significantly
different
than
continued
Duncan
test.
results
showed
encapsulated
(v/v)
(w/v)
had
highest
process
during
28
days
storage
at
low
temperatures
(4°C)
each
amounted
92.66%
90.38%.
best
0.5%
bile
salts
pH
(pH
2).
result
protected
bacterium.
Revista de Nutrición Clínica y Metabolismo,
Journal Year:
2024,
Volume and Issue:
7(1)
Published: April 4, 2024
El
síndrome
metabólico
se
define
como
una
serie
de
anormalidades
metabólicas
que
predisponen
a
los
individuos
desarrollar
diabetes
y/o
enfermedades
cardiovasculares.
La
prevalencia
aumenta
según
factores
la
edad,
el
sexo,
etnia
e
inclusive
estado
microbiota
intestinal.
correcto
y
equilibrio
microbiota,
“eubiosis”,
o
su
desequilibrio,
“disbiosis”,
condiciona
funcionamiento
distintos
mecanismos
control
saciedad,
homeostasis
glucosa
insulina,
inflamación
formación
placa
ateroma.
De
esta
manera,
siguiente
revisión
tiene
objetivo
identificar
relación
entre
intestinal
relacionados
con
metabólico.
Frontiers in Microbiology,
Journal Year:
2024,
Volume and Issue:
15
Published: July 3, 2024
To
exert
their
beneficial
effects,
microorganisms
used
in
live
bacteria-containing
products
must
be
viable
and
present
certain
amounts.
In
this
study,
we
developed
a
viability
assay
based
on
quantitative
PCR
coupled
with
propidium
monoazide
for
the
identification
enumeration
of
Lactobacillus
acidophilus
Bifidobacterium
bifidum
.
order
to
optimize
protocol,
thermal
inactivation
conditions
two
target
PMA
concentration
inhibiting
DNA
amplification
from
dead
cells
while
allowing
it
were
first
determined.
The
viability-PCR
protocol
was
then
applied
analyze
commercial
product
containing
microorganisms.
quantities
both
determined
using
tested
significantly
higher
than
those
obtained
standard
plate
count,
suggesting
presence
bacteria
but
non-culturable
physiological
state.
Moreover,
lower
amounts
detected
compared
achieved
PCR,
possibly
because
samples.
Our
results
suggest
that
method
proposed
here
is
suitable
alternative
rapid
accurate
quantification
assessment
L.
B.
could
easily
adopted
quality
control
screening
products.
