Combining forced limb use with Ninjin'yoeito treatment prevents atrophy in fast-twitch muscles and promotes functional restoration after hemorrhagic stroke in rat models DOI Creative Commons

Naoki Tajiri,

Shinya Ueno,

Dewi Mustika

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

Abstract Rehabilitative training incorporating forced limb use (FLU) following intracerebral hemorrhagic stroke (ICH) enhances functional recovery of skilled reaching in rats. Given that Ninjin'yoeito (NYT) influences both cerebral and muscular systems, this study aimed to investigate whether the combined application FLU NYT could yield superior compared alone. The ICH model was established by collagenase injection, subject administered from day 1 after (D1) for 7 days 1% chow until D56. combination resulted significantly enhanced motor deficit scores at D28 D56 with only, although score comparable group. group exhibited increased total walking distance a higher number center entrances open-field test D28. Retrograde labeling corticospinal neurons FluoroGold (FG) revealed no significant increase FG-positive cells cortex Anterograde biotinylated dextran amine demonstrated bouton-like varicosities red nucleus, similar group, alone did not positive cells. Specific atrophy MHC IIb-positive muscles mitigated changes were observed either or groups. These findings indicate contributes ICH, mitigating fast-twitch muscles.

Language: Английский

Combining forced limb use with Ninjin'yoeito treatment prevents atrophy in fast-twitch muscles and promotes functional restoration after hemorrhagic stroke in rat models DOI Creative Commons

Naoki Tajiri,

Shinya Ueno,

Dewi Mustika

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

Abstract Rehabilitative training incorporating forced limb use (FLU) following intracerebral hemorrhagic stroke (ICH) enhances functional recovery of skilled reaching in rats. Given that Ninjin'yoeito (NYT) influences both cerebral and muscular systems, this study aimed to investigate whether the combined application FLU NYT could yield superior compared alone. The ICH model was established by collagenase injection, subject administered from day 1 after (D1) for 7 days 1% chow until D56. combination resulted significantly enhanced motor deficit scores at D28 D56 with only, although score comparable group. group exhibited increased total walking distance a higher number center entrances open-field test D28. Retrograde labeling corticospinal neurons FluoroGold (FG) revealed no significant increase FG-positive cells cortex Anterograde biotinylated dextran amine demonstrated bouton-like varicosities red nucleus, similar group, alone did not positive cells. Specific atrophy MHC IIb-positive muscles mitigated changes were observed either or groups. These findings indicate contributes ICH, mitigating fast-twitch muscles.

Language: Английский

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