International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 290, P. 138999 - 138999
Published: Dec. 20, 2024
Language: Английский
Insect Science, Journal Year: 2025, Volume and Issue: unknown
Published: March 6, 2025
Silkworm is a typical monophagous insect that can only feed on fresh mulberry leaves. The mechanism for this nature not fully understood. One bitter gustatory receptor (GR) GR66 located the maxilla of mouthpart has been reported to be an important factor influencing feeding preference silkworm. However, mutants nonhost plant was very low, suggesting other factors related silkworm monophagy need further explored. In study, 10 GRs were screened out based their specific high expression in silkworm, and 4 most expressed (GR15, GR43, GR69, GR66) knocked by clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR-associated nuclease 9. Feeding experiments showed except GR69-/-, host range GR43-/-, GR15-/-, GR66-/- expanded remarkably, both GR43-/- revealed highest plants. Moreover, strict leaves almost eliminated double mutant silkworms GR66-/-. These results imply it likely variety different are involved determining larvae. addition, GR15 mutation significantly inhibited ovarian development, resulting significant decrease oviposition. findings enhance our understanding provide possibility molecular breeding polyphagous future.
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 6, 2025
ABSTRACT Artificial sweeteners are highly sweet, non-nutritive compounds that have become increasingly popular over recent decades despite research suggesting their consumption has unintended consequences. Specifically, there is evidence some of these chemicals interact with bitter taste receptors, implying likely generate complex chemosensory signals. Here, we report the basic sensory characteristics in Drosophila , a common model system used to study impacts diet, and find all noncaloric inhibited appetitive feeding responses at higher concentrations. At cellular level, found sucralose rebaudioside A co-activated sweet gustatory receptor neurons (GRNs), two populations reciprocally impact behavior, while aspartame only activated cells. We assessed behavioral co-activation low concentrations signal high aversion. Finally, silencing GRNs reduced aversive elicited by significantly increased behaviors. Together, conclude artificial more than “sweetness” alone, this behaviorally relevant effects on may help flies flexibly respond unique compounds.
Language: Английский
Citations
0
Insects, Journal Year: 2024, Volume and Issue: 15(9), P. 653 - 653
Published: Aug. 30, 2024
Population replacement refers to the process by which a wild-type population of insect pests is replaced possessing modified traits or abilities. Effective necessitates gene drive system capable spreading desired genes within natural populations, operating under principles akin super-Mendelian inheritance. Consequently, releasing small number genetically edited insects could potentially achieve control objectives. Currently, several approaches are exploration, including newly adapted CRISPR-Cas genome editing system. Multiple studies investigating methods engineer that incapable causing crop damage transmitting vector-borne diseases, with notable successful examples documented. This review summarizes recent advancements in realm and provides insights into research methodologies, testing protocols, implementation strategies for techniques. The also discusses emerging trends prospects establishing genetic tools pest management.
Language: Английский
Citations
1
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9513 - 9513
Published: Sept. 1, 2024
The northern bark beetle,
Language: Английский
Citations
1
International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 290, P. 138999 - 138999
Published: Dec. 20, 2024
Language: Английский
Citations
0