Gegen-Sangshen oral liquid and its active fractions mitigate alcoholic liver disease in mice through repairing intestinal epithelial injury and regulating gut microbiota DOI Creative Commons

Shulin Wei,

Mingxing Li, Long Zhao

et al.

Chinese Medicine, Journal Year: 2024, Volume and Issue: 19(1)

Published: Dec. 23, 2024

Abstract Background Liuweizhiji Gegen-Sangshen oral liquid (LGS), as a Chinese medicinal preparation, is developed from Traditional formula consisting of six herbs, including Puerariae lobatae radix, Hoveniae semen, Imperatae rhizoma, Crataegi fructus, Mori fructus and Canarli has been extensively utilized in the prevention treatment alcoholic liver disease (ALD) clinically. Previous study demonstrated that LGS dose-dependently mitigated ALD rat models. However, whether how main characteristic constituents (the flavonoid polysaccharide fractions, LGSF LGSP) contribute to anti-ALD effect remains unclear. This aimed assess its fractions (LGSF murine model explore underlying mechanisms. Methods mouse was constructed using chronic binge ethanol feeding method. Biochemical determinations AST, ALT, TC, TG, ADH, ALDH, HDL, LDL, IL-1β, IL-6, TNF-α were performed corresponding kits. Histopathological examination intestinal sections conducted based on H&E staining. Lipid accumulation hepatocytes evaluated by oil red O Ethanol metabolism assessed determining activity ADH ALDH enzymes. Intestinal barrier function analyzed immunohistochemistry analysis ZO-1 occludin immunofluorescence epithelial markers, Lgr5, Muc2, Lyz1. apoptosis detected TUNEL Mouse fecal microbiota alterations 16S rRNA sequencing. An vitro injury established developing TNF-α-induced 3D-cultured organoids. In culture specific bacterial strains performed. Results The results showed significantly alleviated mice through attenuating hepatic inflammation, improving steatosis promoting metabolism. Notably, LGS, LGSP, damage maintained mice. protection generally more obvious than positive drug meltadosine. Further promoted repair via Lgr5 + stem cell mediated regeneration LGSF, other had better vitro. Moreover, remarkably gut dysbiosis at least partially recovery alcohol-induced microbial changes induction groups. indicated impact growth. but not growth Lactobacillus . Similarly, LGSP increased proliferation Bacteroides sartorii , minimal effect. all Bacillus coagulans Bifidobacterium adolescentis bifidum Dubosiella newyorkensis no Conclusions exerts regulating gut-liver axis, are responsible for protective

Language: Английский

Quality control and spectrum–effect relationship of Crataegus pinnatifida polysaccharide from different habitats DOI
Yongshuai Jing, Qiyuan Han, Ziyan Li

et al.

Natural Product Research, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 12

Published: March 13, 2025

The polysaccharides of Crataegus pinnatifida (CPP) from ten habitats were prepared through water abstraction and alcohol precipitation. Subsequently, an analysis was conducted on their physicochemical properties molecular structure. establishment multidimensional fingerprints is based instrumental methods. Through the evaluation its antioxidant activity, spectrum-effect relationship studied. GalA in monosaccharide constitution components with a weight (Mw) ranging 2.07 × 106 Da to 5.57 have positive correlation scavenging DPPH free radicals. This can furnish preparatory basis data reference for establishing quality assurance standard CPP clarifying structure-activity relationship. At same time, it provides guidance cultivation, processing, development utilisation hawthorn promotes sustainable industry.

Language: Английский

Citations

0
Structural Elucidation and NMR Spectral Assignments of New Diphenyl Ether Derivatives From Liuweizhiji Gegen‐Sangshen Oral Liquid DOI
Jianguo Cui,

Xin Zhou,

Baorui Teng

et al.

Magnetic Resonance in Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 18, 2024

ABSTRACT Chemical investigation of the Liuweizhiji Gegen‐Sangshen oral liquid afforded one new diphenyl ether derivative ( 1 ), together with known compound 2 ). Their structures were established by 1D and 2D NMR, HR‐ESI‐MS spectroscopic analysis absolute configuration was confirmed ECD calculation. Compounds evaluated for cytotoxic activities, compounds showed weak activities towards HepG2 human liver cancer cells, IC 50 values 97.3 79.6 μM, respectively.

Language: Английский

Citations

0
Liuweizhiji Gegen-Sangshen beverage protects against alcoholic liver disease in mice through the gut microbiota mediated SCFAs/GPR43/GLP-1 pathway DOI Creative Commons

Minji Tang,

Long Zhao, Fuchun Huang

et al.

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: Dec. 13, 2024

Introduction Alcoholic liver disease (ALD) is a pathological state of the caused by longterm alcohol consumption. Recent studies have shown that modulation gut microbiota and its metabolic products, specifically short-chain fatty acids (SCFAs), exert critical role in evolution progression ALD. The Liuweizhiji Gegen-Sangshen beverage (LGS), as functional China, derived from traditional Chinese herbal formula has been clinically applied for ALD treatment, demonstrating significant efficacy. However, underlying mechanisms LGS alleviating involving regulation remain unknown. Methods In this study, an murine model based on National Institute Alcohol Abuse Alcoholism (NIAAA) method was established. Results results showed oral treatment dose-dependently alleviated alcoholinduced injury inflammation mice through decreasing levels ALT, AST proinflammatory cytokines (TNF-α, IL-6, IL-1β). significantly improved steatosis, enhanced activities metabolizing enzymes (ALDH ADH), reduced CYP2E1 activity. Notably, regarding most detected indices, effect (particularly at medium high dose) comparable to positive drug MTDX. Moreover, had favorable maintaining intestinal barrier function reducing epithelial increasing expression occludin. 16S rRNA sequencing remarkably modulated structure via recovering alcohol-induced microbial changes mediating enrichment several bacterial genera ( Alloprevotella , Monoglobus Erysipelatoclostridium Parasutterella, Harryflintia unclassified_c_Clostridia ). Further study revealed increased production SCFAs hexanoic acid cecum, promoted alcohol-mediated reduction GRP43 ileum, serum GLP-1 level. Discussion Overall, exerts remarkable protective mediated specific GPR43/GLP-1 pathway.

Language: Английский

Citations

0
Gegen-Sangshen oral liquid and its active fractions mitigate alcoholic liver disease in mice through repairing intestinal epithelial injury and regulating gut microbiota DOI Creative Commons

Shulin Wei,

Mingxing Li, Long Zhao

et al.

Chinese Medicine, Journal Year: 2024, Volume and Issue: 19(1)

Published: Dec. 23, 2024

Abstract Background Liuweizhiji Gegen-Sangshen oral liquid (LGS), as a Chinese medicinal preparation, is developed from Traditional formula consisting of six herbs, including Puerariae lobatae radix, Hoveniae semen, Imperatae rhizoma, Crataegi fructus, Mori fructus and Canarli has been extensively utilized in the prevention treatment alcoholic liver disease (ALD) clinically. Previous study demonstrated that LGS dose-dependently mitigated ALD rat models. However, whether how main characteristic constituents (the flavonoid polysaccharide fractions, LGSF LGSP) contribute to anti-ALD effect remains unclear. This aimed assess its fractions (LGSF murine model explore underlying mechanisms. Methods mouse was constructed using chronic binge ethanol feeding method. Biochemical determinations AST, ALT, TC, TG, ADH, ALDH, HDL, LDL, IL-1β, IL-6, TNF-α were performed corresponding kits. Histopathological examination intestinal sections conducted based on H&E staining. Lipid accumulation hepatocytes evaluated by oil red O Ethanol metabolism assessed determining activity ADH ALDH enzymes. Intestinal barrier function analyzed immunohistochemistry analysis ZO-1 occludin immunofluorescence epithelial markers, Lgr5, Muc2, Lyz1. apoptosis detected TUNEL Mouse fecal microbiota alterations 16S rRNA sequencing. An vitro injury established developing TNF-α-induced 3D-cultured organoids. In culture specific bacterial strains performed. Results The results showed significantly alleviated mice through attenuating hepatic inflammation, improving steatosis promoting metabolism. Notably, LGS, LGSP, damage maintained mice. protection generally more obvious than positive drug meltadosine. Further promoted repair via Lgr5 + stem cell mediated regeneration LGSF, other had better vitro. Moreover, remarkably gut dysbiosis at least partially recovery alcohol-induced microbial changes induction groups. indicated impact growth. but not growth Lactobacillus . Similarly, LGSP increased proliferation Bacteroides sartorii , minimal effect. all Bacillus coagulans Bifidobacterium adolescentis bifidum Dubosiella newyorkensis no Conclusions exerts regulating gut-liver axis, are responsible for protective

Language: Английский

Citations

0