Macromolecular Research, Journal Year: 2025, Volume and Issue: unknown
Published: April 11, 2025
Language: Английский
Biomacromolecules, Journal Year: 2023, Volume and Issue: 24(7), P. 3086 - 3093
Published: June 21, 2023
Bioprinting of hydrogel-based bioinks can allow for the fabrication elaborate, cell-laden 3D structures. In addition to providing an adequate extracellular matrix mimetic environment and high cell viability, hydrogels must offer facile extrusion through printing nozzle retain shape printed structure. We demonstrate a strategy incorporate cellulose oxalate nanofibrils in hyaluronan-based generate shear thinning that allowed free-standing multilayer structures, covalently cross-linked after bioprinting, yielding long-term stability. The storage modulus was tunable between 0.5 1.5 kPa. nanocellulose containing showed good biocompatibility, with viability primary human dermal fibroblasts above 80% at day 7 seeding. cells were also shown tolerate process well, 24 h printing. anticipate this hydrogel system find broad use as bioink produce complex geometries support growth.
Language: Английский
Citations
11
Biomedicines, Journal Year: 2024, Volume and Issue: 12(1), P. 224 - 224
Published: Jan. 19, 2024
Biocompatibility testing of materials is carried out in 2D cell cultures or animal models despite serious limitations. 3D skin equivalents are advanced vitro for human skin. Silicone has been shown to be noncytotoxic but capable eliciting an immune response. Our aim was (1) establish a equivalent (2) assess the proinflammatory properties silicone. We developed coculture keratinocytes and fibroblasts resulting with implant using samples from breast implant. Samples without silicone were studied histologically immunohistochemically comparison native samples. Cytotoxicity assessed via LDH-assay, cytokine response ELISA. Histologically, our had four-layered epidermal dermal component. The presence tight junctions demonstrated immunofluorescence. only difference implants thinning. Implanting did not cause more death, however, inflammatory triggered. able organotypical implant, which can utilised studies on biocompatibility materials. This first integration into confirmed previous findings being non-cell-toxic exerting effect.
Language: Английский
Citations
4
Current Research in Biotechnology, Journal Year: 2024, Volume and Issue: 7, P. 100210 - 100210
Published: Jan. 1, 2024
Cancer incidence and mortality are increasing globally. immunotherapies, such as immune checkpoint inhibitors adoptive cell therapy, have been recognized a revolutionary treatment approach to combat cancer. However, immunotherapeutic resistance cancer recurrence after immunotherapy alarm us further explore the underlying mechanisms develop new immunotherapies. Experimental models hold great value in research studies deciphering mechanism of tumor initiation growth, drug discovery, evaluation efficacy. The ideal model is expected recapitulate mimic human microenvironment, including biological, physiological, immunologic functionality. each has its pros cons, selection depends on many factors, features, study aims, availability related resources. In this review, we discussed commonly used currently immunotherapy, 2D 3D vitro culture spheroid, organoid, hydrogel model, microfluidic chip, vivo mouse genetically engineered models, chemically induced cell-derived xenograft (CDX) patient-derived (PDX) humanized models. Both preclinical powerful tools for studying but all these their limitations. To promote success clinical advanced systems that can better environment host response preferable options study.
Language: Английский
Citations
4
Cellular Oncology, Journal Year: 2024, Volume and Issue: unknown
Published: May 28, 2024
Cancer is a highly heterogeneous disease, and thus treatment responses vary greatly between patients. To improve therapy efficacy outcome for cancer patients, more representative patient-specific preclinical models are needed. Organoids tumoroids 3D cell culture that typically retain the genetic epigenetic characteristics, as well morphology, of their tissue origin. Thus, they can be used to understand underlying mechanisms initiation, progression, metastasis in physiological setting. Additionally, co-culture methods cancer-associated cells help interplay tumor its microenvironment. In recent years, have already helped refine treatments identify new targets therapy. Advanced culturing systems such chip-based fluidic devices bioprinting combination with been high-throughput applications personalized medicine. Even though organoid tumoroid complex vitro systems, validation results vivo still common practice. Here, we describe how both animal- human-derived novel vulnerabilities currently precision
Language: Английский
Citations
4
Genes, Journal Year: 2025, Volume and Issue: 16(2), P. 180 - 180
Published: Feb. 1, 2025
Prostate cancer (PCa) patients who do not respond to androgen deprivation therapy (ADT), referred as castration-resistant prostate (CRPC), remain a clinical challenge due confirm the aggressive nature of CRPC and its resistance conventional therapies. This study aims investigate potential microRNAs (miRNAs) biomarkers for predicting therapeutic response in patients. We performed miRNA mRNA expression analyses using publicly available datasets applied 3D cell culture models replicate more physiologically relevant tumor conditions. Genetic analysis techniques were employed on data, profiles from examined. Eighteen miRNAs with differential identified between responded favorably abiraterone (responders) those advanced (non-responders). Specifically, such hsa-miR-152-3p hsa-miR-34a-3p found be associated critical pathways, including TGF-β signaling P53, which are linked resistance. Several predictors treatment efficacy, therapies like abiraterone. These results indicate that could serve non-invasive outcomes, facilitating personalized approach treatment. provides novel perspective strategies CRPC, emphasizing role improving precision efficacy this complex disease.
Language: Английский
Citations
0
Biomedicines, Journal Year: 2025, Volume and Issue: 13(2), P. 443 - 443
Published: Feb. 11, 2025
Background/Objectives: Cold atmospheric plasma (CAP) has shown a strong anticancer effect on variety of tumors, presenting new approach for the effective treatment oral squamous cell carcinoma (OSCC), one most prevalent malignant neoplasms with high mortality rate. Here, we aimed to comprehensively investigate antitumor potential two approaches CAP both two-dimensional and three-dimensional OSCC line models, as well analyze whether enhances sensitivity chemotherapy. Methods: An in-house designed needle, helium working gas, was used treat SCC-25 directly or indirectly via plasma-treated medium (PTM). The assessed by measuring viability, apoptosis, adhesion, migration. In addition, combined PTM cisplatin analyzed in tumor spheroids, more complex reliable vitro model. Results: Both treatments showed time-dependent effects affecting their rate apoptosis higher after incubation is mediated intrinsic pathway. By utilizing 3D spheroid model, confirmed additionally demonstrated an increased chemosensitivity PTM-treated cells. Conclusions: results our study illustrate promising avenue application therapeutic option OSCC, either standalone combination cisplatin.
Language: Английский
Citations
0
Archives of Microbiology, Journal Year: 2025, Volume and Issue: 207(4)
Published: Feb. 24, 2025
Language: Английский
Citations
0
Micromachines, Journal Year: 2025, Volume and Issue: 16(3), P. 324 - 324
Published: March 11, 2025
The development of an organ-on-a-chip to reproduce organ functions requires the incorporation a vascular network within tissue transport necessary nutrients. Tissues thicker than 200 µm cannot survive without capillary network, necessitating construction exceeding that thickness. Therefore, we focused on inexpensive and easy-to-fabricate device for thick three-dimensional(3D)-cultured tissues. This does not have conventional pillar array structure, nutrient supply cells from adjacent media channels is obstructed. Additionally, this require expensive soft lithography equipment or high-precision 3D printer fabricate mold. Human glomerular endothelial human dermal fibroblasts were co-cultured using device, (200 thick) was successfully constructed. results study are expected contribute only angiogenesis, but also models networks as well vascularized disease drug screening.
Language: Английский
Citations
0
Advanced Materials Interfaces, Journal Year: 2025, Volume and Issue: unknown
Published: March 12, 2025
Abstract A 3D‐printed origami‐inspired magnetic scaffold has been developed to investigate the influence of physical cues on guided cellular proliferation in a 3D microenvironment. Microscale channels are first constructed and populated with NIH/3T3 fibroblast and/or A549 cancer cell clusters that initially bioprinted within channels. Once these fully populated, permanent magnet is applied fold scaffolds. By varying channel width incorporating an intermediate extracellular matrix hydrogel (IE) layer along origami folding, provides geometric gravitational proliferation. In both monoculture coculture, i) cells tend proliferate more tapered manner, ii) scaffolds enhanced media flow lead higher volume growth, iii) form homogeneous distributions under gravity after dispersion. expansion their seeded increased, facilitating into non‐seeded This offers valuable insights tissue engineering research, serving as versatile tool for examining interactions growth dynamics.
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2524 - 2524
Published: March 12, 2025
Current in vitro methods for intestinal barrier assessment predominantly utilize two-dimensional (2D) membrane inserts standard culture plates, which are widely recognized their inability to replicate the microenvironment critical functionality. Our study focuses on creating an alternative method function by integrating a 3D-printed transwell device with paper-based membrane. Caco-2 cells were grown Matrigel-modified paper membrane, tight junction formation was evaluated using TEER measurements. Neutrophil-like dHL-60 employed neutrophil extracellular trap (NET) experiments. Furthermore, dysfunction demonstrated NET-isolated and Staurosporine interventions. Intestinal characteristics investigated through immunofluorescence staining of specific proteins scanning electron microscopy (SEM). exhibited increased resistance time-dependent manner, consistent images Zonulin Occludens-1 (ZO-1) expression. Interestingly, analysis revealed changes morphology surface villi. These disruptions found alter localization junctions, impacting epithelial polarization Moreover, we successfully permeability FITC-dextran assay. Hence, integrated insert presents straightforward, cost-effective, sustainable platform cell model evaluate function.
Language: Английский
Citations
0