Transdermal delivery of Ultradeformable cationic liposomes complexed with miR211–5p (UCL-211) stabilizes BRAFV600E+ melanocytic nevi

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: unknown, P. 113586 - 113586

Published: March 1, 2025

Language: Английский

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Amelanotic Uveal Melanomas Evaluated by Indirect Ophthalmoscopy Reveal Better Long-Term Prognosis Than Pigmented Primary Tumours—A Single Centre Experience

Cancers, Journal Year: 2022, Volume and Issue: 14(11), P. 2753 - 2753

Published: June 1, 2022

(1) Background: There is a constant search for new prognostic factors that would allow us to accurately determine the prognosis, select type of treatment, and monitor patient diagnosed with uveal melanoma in minimally invasive easily accessible way. Therefore, we decided evaluate role its pigmentation clinical assessment. (2) Methods: The 154 melanomas was assessed by indirect ophthalmoscopy. Two groups tumours were identified: amelanotic pigmented. statistical relationships between these two clinical, pathological parameters long-term survival rate analyzed. (3) Results: 16.9% among all they occurred younger patients (p = 0.022). In pigmented melanomas, unfavourable features such as: epithelioid cells 0.0013), extrascleral extension 0.027), macronucleoli 0.0065), absence BAP1 expression 0.029) statistically more frequently observed. Kaplan−Meier analysis demonstrated significantly better overall 0.017) disease-free < 0.001) rates tumours. However, this relationship significant lower stage (AJCC II), not present larger advanced stages III). (4) Conclusions: results obtained suggested presence ophthalmoscopy associated worse compared lesions. These findings could be useful choice therapeutic follow-up options future.

Language: Английский

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The CNC-family transcription factor Nrf3 coordinates the melanogenesis cascade through macropinocytosis and autophagy regulation

Cell Reports, Journal Year: 2022, Volume and Issue: 42(1), P. 111906 - 111906

Published: Dec. 30, 2022

Melanin is a pigment produced from the amino acid L-tyrosine in melanosomes. The CNC-family transcription factor Nrf3 expressed basal layer of epidermis, where melanocytes reside, but its melanogenic function unclear. Here, we show that regulates macropinocytosis and autophagy to coordinate melanogenesis cascade. In response an exogenous inducer melanin production, forskolin, upregulates core gene circuit, which includes Mitf, Tyr, Tyrp1, Pmel, Oca2. Furthermore, induces expression Cln3, autophagosome-related factor, for precursor uptake by macropinocytosis. Ulk2 Gabarapl2 are also identified as Nrf3-target genes melanosome formation. parallel, prompts autolysosomal degradation melanocyte survival. An endogenous αMSH activates Nrf3-mediated whereas it suppressed HIV-1 protease inhibitor, nelfinavir. These findings indicate significant role anti-melanogenic potential

Language: Английский

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Beyond the Epidermal-Melanin-Unit: The Human Scalp Anagen Hair Bulb Is Home to Multiple Melanocyte Subpopulations of Variable Melanogenic Capacity

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(16), P. 12809 - 12809

Published: Aug. 15, 2023

The visual appearance of humans is derived significantly from our skin and hair color. While melanin epidermal melanocytes protects the damaging effects ultraviolet radiation, biological value pigmentation in follicle, particularly on scalp, less clear. In this study, we explore heterogeneity pigment cells human scalp anagen follicle bulb, a site conventionally viewed to be focused solely production for transfer shaft. Using c-KIT/CD117 microbeads, isolated bulbar c-KIT-positive c-KIT-negative melanocytes. both subpopulations expressed MITF, only fraction SOX10. We further localized melanocyte (expressing c-KIT, SOX10, DCT) that exhibited distinct/variable expression downstream differentiation-associated melanosome markers (e.g., gp100 Melan-A). localization second ‘immature’ SOX10 negative population, which was c-KIT/MITF double-positive, identified outside melanogenic zone most peripheral/proximal matrix. This study describes an approach purifying bulb melanocytes, allowing us identify unexpected levels heterogeneity. function more immature part remains elucidated. Could they in-transit migratory ultimately destined synthesize melanin, or could contribute non-melanogenic ways?

Language: Английский

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Adjuvant PD-1 Checkpoint Inhibition in Early Cutaneous Melanoma: Immunological Mode of Action and the Role of Ultraviolet Radiation

Cancers, Journal Year: 2024, Volume and Issue: 16(8), P. 1461 - 1461

Published: April 11, 2024

Melanoma ranks as the fifth most common solid cancer in adults worldwide and is responsible for a significant proportion of skin-tumor-related deaths. The advent immune checkpoint inhibition with anti-programmed death protein-1 (PD-1) antibodies has revolutionized adjuvant treatment high-risk, completely resected stage III/IV melanoma. However, not all patients benefit equally. Current strategies improving outcomes involve earlier disease stages (IIB/C) well perioperative approaches. Interfering T-cell exhaustion to counteract evasion immunogenic nature melanoma key anti-PD-1 effectiveness. Yet, biological rationale efficacy clinically tumor-free remains be fully elucidated. High-dose intermittent sun exposure (sunburn) well-known primary risk factor melanomagenesis. Also, ultraviolet radiation (UVR)-induced immunosuppression may impair anti-cancer surveillance. In this review, we summarize current knowledge about blockade, including characterization main cell types likely its efficacy. conclusion, propose that local systemic immunosuppression, some extent UVR-mediated, can restored by therapy, consequently boosting anti-melanoma surveillance elimination residual clones.

Language: Английский

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Beyond the Epidermal-Melanin-Unit: The Human Scalp Anagen Hair Bulb Is Home to Multiple Melanocyte Subpopulations of Variable Melanogenic Capacity

Published: July 6, 2023

The visual appearance of humans derives significantly from our skin and hair color. While melanin epidermal melanocytes protects the damaging effects ultraviolet radiation, biological value pigmentation in follicle, particularly scalp, is less clear. In this study, we explore heterogeneity pigment cells human scalp anagen follicle bulb, a site conventionally viewed to be focused solely on production for transfer shaft. Using c-KIT/CD117 microbeads, isolated bulbar c-KIT-positive c-KIT-negative melanocytes. both subpopulations expressed MITF, only fraction SOX10. We further localized melanocyte (expressing c-KIT, SOX10, DCT) that exhibited distinct/variable expression downstream differentiation-associated melanosome markers (e.g., gp100 Melan-A). Localization second &lsquo;immature&rsquo; SOX10 negative population, which was c-KIT/MITF double positive, identified outside melanogenic zone most peripheral/proximal matrix. These studies describe an approach purifying bulb melanocytes, allowing us identify unexpected levels heterogeneity. function more immature part remains elucidated. Could they in-transit migratory destined synthesize mela-nin ultimately or could contribute non-melanogenic ways.

Language: Английский

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Combined signature of G protein-coupled receptors and tumor microenvironment provides a prognostic and therapeutic biomarker for skin cutaneous melanoma

Journal of Cancer Research and Clinical Oncology, Journal Year: 2023, Volume and Issue: 149(20), P. 18135 - 18160

Published: Nov. 25, 2023

Language: Английский

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Understanding the charismatic potential of nanotechnology to treat skin carcinoma

Medical Oncology, Journal Year: 2023, Volume and Issue: 41(1)

Published: Dec. 19, 2023

Language: Английский

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Achievements and prospects in skin melanoma 3D modeling

Russian Journal of Preventive Medicine, Journal Year: 2024, Volume and Issue: 27(4), P. 103 - 103

Published: Jan. 1, 2024

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Dangerous liaisons: Loss of keratinocyte control over melanocytes in melanomagenesis

BioEssays, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 4, 2024

Abstract Melanomas arise from transformed melanocytes, positioned at the dermal‐epidermal junction in basal layer of epidermis. Melanocytes are completely surrounded by keratinocyte neighbors, with which they communicate through direct contact and paracrine signaling to maintain normal growth control homeostasis. UV radiation sunlight reshapes this communication network drive a protective tanning response. However, repeated rounds sun exposure result accumulation mutations melanocytes that have been considered as primary drivers melanoma initiation progression. It is now clear not sufficient tumor formation—the environment plays critical role. This review focuses on changes melanocyte‐keratinocyte contribute progression, particular focus recent mechanistic insights lay foundation for developing new ways intercept development.

Language: Английский

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