Experimental Dermatology,
Journal Year:
2023,
Volume and Issue:
32(7), P. 1063 - 1071
Published: June 7, 2023
Abstract
A
direct
contact
co‐culture
of
skin
explants
to
SZ95
sebocytes
(3D‐SeboSkin)
has
been
shown
preserve
the
integrity
epidermal
keratinocytes
and
dermis.
In
this
study,
properties
melanocytes
were
evaluated
in
same
3D
SeboSkin
ex
vivo
model.
Skin
(
n
=
6)
maintained
3D‐SeboSkin
model,
fibroblasts
alone
serum‐free
medium
(SFM).
Histopathological,
immunohistochemical,
apoptosis
oil
red
staining
evaluations
performed
at
Days
0
6
incubation.
Results
revealed
preservation
prominent
proliferation
basal
addition
dermal
collagen
vasculature
Day
culture
model
a
lesser
extent
with
but
not
SFM
alone.
Melan‐A+/Ki67‐
remained
attached
dermis
even
sites
detachment
three
explant
models
tested.
However,
number
was
significantly
conserved
cultures
comparison
p
<
0.05),
whereas
no
difference
found
fibroblasts.
Few
DAPI/TUNEL+
apoptotic
could
mostly
be
observed
SFM‐incubated
explants.
Furthermore,
only
exhibited
increased
lipogenesis
accumulation
abundant
lipid
droplets.
These
results
denote
that
yielded
significant
hence
it
is
optimal
for
studies
abnormalities
pigmentation,
melanocyte
neoplasms
effects
different
hormones,
cytokines,
carcinogens
various
therapeutics
pattern
recapitulates
environment.
Abstract
Small
non-coding
RNAs
(e.g.
siRNA,
miRNA)
are
involved
in
a
variety
of
melanocyte-associated
skin
conditions
and
act
as
drivers
for
alterations
gene
expression
within
melanocytes.
These
molecular
changes
can
potentially
affect
the
cellular
stability
melanocytes
promote
their
oncogenic
transformation.
Thus,
small
be
considered
therapeutic
targets
these
conditions,
however,
topical
delivery
to
through
epidermal
barrier
is
challenging.
We
synthesized
extensively
evaluated
ultradeformable
cationic
liposome
(UCLs)
carriers
complexed
with
synthetic
microRNAs
(miR211-5p;
UCL-211)
transdermal
UCL-211
complexes
were
characterized
physicochemical
properties,
encapsulation
efficiency,
deformability,
which
revealed
significant
advantage
over
conventional
liposomal
carriers.
Increased
miR211-5p
stabilizes
melanocytic
nevi
keeps
them
growth-arrested
state.
did
comprehensive
assessment
delivery,
biological
activity
carried
by
vitro
permeation
epidermis
intact
using
ex
vivo
human
tissue
explants.
also
demonstrated,
,
that
stabilized
BRAFV600E+
benign
Experimental Dermatology,
Journal Year:
2023,
Volume and Issue:
32(7), P. 1063 - 1071
Published: June 7, 2023
Abstract
A
direct
contact
co‐culture
of
skin
explants
to
SZ95
sebocytes
(3D‐SeboSkin)
has
been
shown
preserve
the
integrity
epidermal
keratinocytes
and
dermis.
In
this
study,
properties
melanocytes
were
evaluated
in
same
3D
SeboSkin
ex
vivo
model.
Skin
(
n
=
6)
maintained
3D‐SeboSkin
model,
fibroblasts
alone
serum‐free
medium
(SFM).
Histopathological,
immunohistochemical,
apoptosis
oil
red
staining
evaluations
performed
at
Days
0
6
incubation.
Results
revealed
preservation
prominent
proliferation
basal
addition
dermal
collagen
vasculature
Day
culture
model
a
lesser
extent
with
but
not
SFM
alone.
Melan‐A+/Ki67‐
remained
attached
dermis
even
sites
detachment
three
explant
models
tested.
However,
number
was
significantly
conserved
cultures
comparison
p
<
0.05),
whereas
no
difference
found
fibroblasts.
Few
DAPI/TUNEL+
apoptotic
could
mostly
be
observed
SFM‐incubated
explants.
Furthermore,
only
exhibited
increased
lipogenesis
accumulation
abundant
lipid
droplets.
These
results
denote
that
yielded
significant
hence
it
is
optimal
for
studies
abnormalities
pigmentation,
melanocyte
neoplasms
effects
different
hormones,
cytokines,
carcinogens
various
therapeutics
pattern
recapitulates
environment.
